Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers

Aging is attributed to both genetic and environmental factors. Occupational exposure is one of the environmental factors with potential genotoxic effects. Researchers try to determine factors involved in genetic damages at hazards exposure that could accelerate aging. Cytochrome P450 2E1 (CYP2E1) ge...

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Main Authors: Eshkoor, Sima Ataollahi, Ismail, Patimah, Abdul Rahman, Sabariah, Adon, Mohd Yusoff, Vasudevan, Ramachandran
Format: Article
Published: Taylor & Francis 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29808/
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author Eshkoor, Sima Ataollahi
Ismail, Patimah
Abdul Rahman, Sabariah
Adon, Mohd Yusoff
Vasudevan, Ramachandran
author_facet Eshkoor, Sima Ataollahi
Ismail, Patimah
Abdul Rahman, Sabariah
Adon, Mohd Yusoff
Vasudevan, Ramachandran
author_sort Eshkoor, Sima Ataollahi
building UPM Institutional Repository
collection Online Access
description Aging is attributed to both genetic and environmental factors. Occupational exposure is one of the environmental factors with potential genotoxic effects. Researchers try to determine factors involved in genetic damages at hazards exposure that could accelerate aging. Cytochrome P450 2E1 (CYP2E1) gene contributes in activation and detoxification of the environmental hazards. This polymorphism plays an important role in susceptibility of inter-individuals to DNA damage at the occupational exposure. The current study evaluated the possible influence of this gene polymorphism in aging by genomic damages through the biomarkers alterations of micronuclei (MN), comet tail length and telomere length shortening at the exposure. In this study, buccal cells were collected from the oral cavity of exposed workers and non-exposed controls. The CYP2E1 genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The wild genotype significantly affected MN frequency (p=0.007) and relative telomere length (p=0.047) in the older group of workers. It was concluded that the interaction of gene polymorphism and exposure enhances DNA damage and accelerates aging consequently.
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spelling upm-298082016-02-19T02:25:46Z http://psasir.upm.edu.my/id/eprint/29808/ Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers Eshkoor, Sima Ataollahi Ismail, Patimah Abdul Rahman, Sabariah Adon, Mohd Yusoff Vasudevan, Ramachandran Aging is attributed to both genetic and environmental factors. Occupational exposure is one of the environmental factors with potential genotoxic effects. Researchers try to determine factors involved in genetic damages at hazards exposure that could accelerate aging. Cytochrome P450 2E1 (CYP2E1) gene contributes in activation and detoxification of the environmental hazards. This polymorphism plays an important role in susceptibility of inter-individuals to DNA damage at the occupational exposure. The current study evaluated the possible influence of this gene polymorphism in aging by genomic damages through the biomarkers alterations of micronuclei (MN), comet tail length and telomere length shortening at the exposure. In this study, buccal cells were collected from the oral cavity of exposed workers and non-exposed controls. The CYP2E1 genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The wild genotype significantly affected MN frequency (p=0.007) and relative telomere length (p=0.047) in the older group of workers. It was concluded that the interaction of gene polymorphism and exposure enhances DNA damage and accelerates aging consequently. Taylor & Francis 2013-05 Article PeerReviewed Eshkoor, Sima Ataollahi and Ismail, Patimah and Abdul Rahman, Sabariah and Adon, Mohd Yusoff and Vasudevan, Ramachandran (2013) Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers. Toxicology Mechanisms and Methods, 23 (4). pp. 217-222. ISSN 1537-6516; ESSN: 1537-6524 http://www.tandfonline.com/doi/abs/10.3109/15376516.2012.743637?journalCode=itxm20 10.3109/15376516.2012.743637
spellingShingle Eshkoor, Sima Ataollahi
Ismail, Patimah
Abdul Rahman, Sabariah
Adon, Mohd Yusoff
Vasudevan, Ramachandran
Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers
title Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers
title_full Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers
title_fullStr Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers
title_full_unstemmed Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers
title_short Contribution of CYP2E1 polymorphism to aging in the mechanical workshop workers
title_sort contribution of cyp2e1 polymorphism to aging in the mechanical workshop workers
url http://psasir.upm.edu.my/id/eprint/29808/
http://psasir.upm.edu.my/id/eprint/29808/
http://psasir.upm.edu.my/id/eprint/29808/