Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
Rattus argentiventer (Rice Field Rat) is responsible for destruction of paddy. The mammal is known for its rapid reproductive potential which can be a target for biological control of this species. Phospholipase C-zeta (PLC ζ) is a specific enzyme found in sperm of mammals responsible for triggering...
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| Format: | Article |
| Language: | English |
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International Organization Of Scientific Research (IOSR)
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29791/ http://psasir.upm.edu.my/id/eprint/29791/1/Detection%20of%20PLC.pdf |
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| author | A. S., Fadzlina A. M. N., Nasyriq M. N., Noor Faezah Lee, L. H. Cheah, Yoke Kqueen Sukardi, Sabrina |
| author_facet | A. S., Fadzlina A. M. N., Nasyriq M. N., Noor Faezah Lee, L. H. Cheah, Yoke Kqueen Sukardi, Sabrina |
| author_sort | A. S., Fadzlina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Rattus argentiventer (Rice Field Rat) is responsible for destruction of paddy. The mammal is known for its rapid reproductive potential which can be a target for biological control of this species. Phospholipase C-zeta (PLC ζ) is a specific enzyme found in sperm of mammals responsible for triggering calcium oscillations leading to egg activation during fertilization. It facilitates the first step of egg activation causing egg division and subsequent development into an embryo. The method used in this study was to identify PLC gene fragments from the testis of Rattus argentiventer using two-step Reverse Transcriptase Polymerase Chain Reaction (PCR) and quantified with Real Time Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR). Following that, sequencing of PLC was performed. The identified sequence was then keyed in into the BLAST
portal in NCBI (National Centre of Bioinformatics) for comparison with a standard PLC sequence from Rattus
norvegicus for sequence alignment. The result showed that PLCζ was present in Rattus argentiventer and qRTPCR could quantify the amount of PLCζ available.As such this would give additional information of detection and quantification techniques for rapid identification and detection of PLCζ to be carried out in the approach of controlling the rapid growth of Rattus argentiventer population.
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| first_indexed | 2025-11-15T09:03:39Z |
| format | Article |
| id | upm-29791 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:03:39Z |
| publishDate | 2013 |
| publisher | International Organization Of Scientific Research (IOSR) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-297912015-09-02T07:43:18Z http://psasir.upm.edu.my/id/eprint/29791/ Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR A. S., Fadzlina A. M. N., Nasyriq M. N., Noor Faezah Lee, L. H. Cheah, Yoke Kqueen Sukardi, Sabrina Rattus argentiventer (Rice Field Rat) is responsible for destruction of paddy. The mammal is known for its rapid reproductive potential which can be a target for biological control of this species. Phospholipase C-zeta (PLC ζ) is a specific enzyme found in sperm of mammals responsible for triggering calcium oscillations leading to egg activation during fertilization. It facilitates the first step of egg activation causing egg division and subsequent development into an embryo. The method used in this study was to identify PLC gene fragments from the testis of Rattus argentiventer using two-step Reverse Transcriptase Polymerase Chain Reaction (PCR) and quantified with Real Time Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR). Following that, sequencing of PLC was performed. The identified sequence was then keyed in into the BLAST portal in NCBI (National Centre of Bioinformatics) for comparison with a standard PLC sequence from Rattus norvegicus for sequence alignment. The result showed that PLCζ was present in Rattus argentiventer and qRTPCR could quantify the amount of PLCζ available.As such this would give additional information of detection and quantification techniques for rapid identification and detection of PLCζ to be carried out in the approach of controlling the rapid growth of Rattus argentiventer population. International Organization Of Scientific Research (IOSR) 2013-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29791/1/Detection%20of%20PLC.pdf A. S., Fadzlina and A. M. N., Nasyriq and M. N., Noor Faezah and Lee, L. H. and Cheah, Yoke Kqueen and Sukardi, Sabrina (2013) Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR. IOSR Journal of Pharmacy and Biological Sciences, 8 (3). pp. 65-74. ISSN 2319-7676; ESSN: 2278-3008 |
| spellingShingle | A. S., Fadzlina A. M. N., Nasyriq M. N., Noor Faezah Lee, L. H. Cheah, Yoke Kqueen Sukardi, Sabrina Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR |
| title | Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR |
| title_full | Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR |
| title_fullStr | Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR |
| title_full_unstemmed | Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR |
| title_short | Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR |
| title_sort | detection of plc-ζ from testis of rattus argentiventer(rice-field rat) using rt-pcr and qrt-pcr |
| url | http://psasir.upm.edu.my/id/eprint/29791/ http://psasir.upm.edu.my/id/eprint/29791/1/Detection%20of%20PLC.pdf |