Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops
This study involves the induced production of α-tocotrienol by the co-suppression of tocopherol cyclase gene. The Tocopherol/tocotrienol Cyclase (TC) is a key enzyme involved in the biosynthesis of α-tocotrienol. The partial conserved cDNA of TC gene isolated from the oil palm (Elaeis guineensis) th...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Science Publications
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/1/ajbbsp.2013.355.364.pdf |
| _version_ | 1848846491667398656 |
|---|---|
| author | Munusamy, Umaiyal Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Khaza'ai, Huzwah Lai, Mun Seong |
| author_facet | Munusamy, Umaiyal Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Khaza'ai, Huzwah Lai, Mun Seong |
| author_sort | Munusamy, Umaiyal |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study involves the induced production of α-tocotrienol by the co-suppression of tocopherol cyclase gene. The Tocopherol/tocotrienol Cyclase (TC) is a key enzyme involved in the biosynthesis of α-tocotrienol. The partial conserved cDNA of TC gene isolated from the oil palm (Elaeis guineensis) that shared greater than 75% sequence identity with TC sequences from other plant species was used to silence TC gene expression in Amaranthus sp. and Allium porrum leaves by co-suppression strategies. This was carried out using recombinant vector constructs designated as p4a11 and p4c9 containing TC driven by the maize Ubiquitin promoter (Ubi1P) and the oil palm leaf-specific promoter (LHCB), respectively. In the Amaranthus sp. leaves infiltrated with p4a11, the α-tocopherol suppression was 85.0, 71.0 and 74.0% on day three, five and seven, respectively and complete suppression was observed in the leaves infiltrated with p4c9. Whereas, only 11.0, 7.0 and 29.0% suppression was detected in Allium porrum leaves infiltrated with p4a11 and 45.0, 4.0 and 57.0% of suppression was observed in Allium porrum infiltrated with p4c9 on day three, five and seven, respectively. Silencing of TC gene driven by LHCB (p4c9) causes the reduction of α-tocopherol production better than by Ubi1P (p4a11). The results have demonstrated that gene silencing through co-suppression is capable of producing α-tocotrienol. However, there is no correlation between the α-tocotrienol production and the α-tocopherol suppression. It has been suggested that the transgene mediated co-suppression mechanism had triggered the vitamin E biosynthesis metabolic pathway to shift towards producing α-tocotrienol. |
| first_indexed | 2025-11-15T09:03:34Z |
| format | Article |
| id | upm-29769 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:03:34Z |
| publishDate | 2013 |
| publisher | Science Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-297692017-12-04T04:37:32Z http://psasir.upm.edu.my/id/eprint/29769/ Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops Munusamy, Umaiyal Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Khaza'ai, Huzwah Lai, Mun Seong This study involves the induced production of α-tocotrienol by the co-suppression of tocopherol cyclase gene. The Tocopherol/tocotrienol Cyclase (TC) is a key enzyme involved in the biosynthesis of α-tocotrienol. The partial conserved cDNA of TC gene isolated from the oil palm (Elaeis guineensis) that shared greater than 75% sequence identity with TC sequences from other plant species was used to silence TC gene expression in Amaranthus sp. and Allium porrum leaves by co-suppression strategies. This was carried out using recombinant vector constructs designated as p4a11 and p4c9 containing TC driven by the maize Ubiquitin promoter (Ubi1P) and the oil palm leaf-specific promoter (LHCB), respectively. In the Amaranthus sp. leaves infiltrated with p4a11, the α-tocopherol suppression was 85.0, 71.0 and 74.0% on day three, five and seven, respectively and complete suppression was observed in the leaves infiltrated with p4c9. Whereas, only 11.0, 7.0 and 29.0% suppression was detected in Allium porrum leaves infiltrated with p4a11 and 45.0, 4.0 and 57.0% of suppression was observed in Allium porrum infiltrated with p4c9 on day three, five and seven, respectively. Silencing of TC gene driven by LHCB (p4c9) causes the reduction of α-tocopherol production better than by Ubi1P (p4a11). The results have demonstrated that gene silencing through co-suppression is capable of producing α-tocotrienol. However, there is no correlation between the α-tocotrienol production and the α-tocopherol suppression. It has been suggested that the transgene mediated co-suppression mechanism had triggered the vitamin E biosynthesis metabolic pathway to shift towards producing α-tocotrienol. Science Publications 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29769/1/ajbbsp.2013.355.364.pdf Munusamy, Umaiyal and Abdullah, Siti Nor Akmar and Abdul Aziz, Maheran and Khaza'ai, Huzwah and Lai, Mun Seong (2013) Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops. American Journal of Biochemistry and Biotechnology, 9 (4). pp. 355-364. ISSN 1553-3468; ESSN: 1558-6332 http://thescipub.com/abstract/10.3844/ajbbsp.2013.355.364 10.3844/ajbbsp.2013.355.364 |
| spellingShingle | Munusamy, Umaiyal Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Khaza'ai, Huzwah Lai, Mun Seong Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops |
| title | Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops |
| title_full | Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops |
| title_fullStr | Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops |
| title_full_unstemmed | Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops |
| title_short | Induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops |
| title_sort | induced production of α-tocotrienol by co-suppression of tocopherol cyclase gene in vegetable crops |
| url | http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/ http://psasir.upm.edu.my/id/eprint/29769/1/ajbbsp.2013.355.364.pdf |