Response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts

This study aimed to determine the total phenolics and antioxidant capacity of defatted dabai parts based on liquid extraction and optimized using response surface methodology (RSM). A two-level factorial design was applied to determine the effect of two independent variables (extraction time: X1 and...

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Main Authors: Khoo, Hock Eng, Azlan, Azrina, Ismail, Amin, Abas, Faridah
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29756/
http://psasir.upm.edu.my/id/eprint/29756/1/Response%20surface%20methodology%20optimization%20for%20extraction%20of%20phenolics%20and%20antioxidant%20capacity%20in%20defatted%20dabai%20parts.pdf
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author Khoo, Hock Eng
Azlan, Azrina
Ismail, Amin
Abas, Faridah
author_facet Khoo, Hock Eng
Azlan, Azrina
Ismail, Amin
Abas, Faridah
author_sort Khoo, Hock Eng
building UPM Institutional Repository
collection Online Access
description This study aimed to determine the total phenolics and antioxidant capacity of defatted dabai parts based on liquid extraction and optimized using response surface methodology (RSM). A two-level factorial design was applied to determine the effect of two independent variables (extraction time: X1 and % methanol: X2) on three response variables (total phenolic content: Y1, total flavonoid/anthocyanin content: Y2 and Trolox equivalent antioxidant capacity: Y3). The optimum conditions for extraction time and percent methanol were 36 min or 1 min and 62.25% or 53% for the defatted dabai pulp or peel, respectively. The RSM optimized extraction was compared with sonication-assisted extraction. Optimization results showed that defatted dabai parts had high total phenolic content and antioxidant capacity. Sonication-assisted extraction utilized the optimized extraction conditions had further increased the total phenolic content and antioxidant capacity of defatted dabai peel, but not in the pulp. Therefore, optimization of different extraction methods for the defatted fruit parts is recommended for future studies.
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spelling upm-297562016-01-21T08:52:32Z http://psasir.upm.edu.my/id/eprint/29756/ Response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts Khoo, Hock Eng Azlan, Azrina Ismail, Amin Abas, Faridah This study aimed to determine the total phenolics and antioxidant capacity of defatted dabai parts based on liquid extraction and optimized using response surface methodology (RSM). A two-level factorial design was applied to determine the effect of two independent variables (extraction time: X1 and % methanol: X2) on three response variables (total phenolic content: Y1, total flavonoid/anthocyanin content: Y2 and Trolox equivalent antioxidant capacity: Y3). The optimum conditions for extraction time and percent methanol were 36 min or 1 min and 62.25% or 53% for the defatted dabai pulp or peel, respectively. The RSM optimized extraction was compared with sonication-assisted extraction. Optimization results showed that defatted dabai parts had high total phenolic content and antioxidant capacity. Sonication-assisted extraction utilized the optimized extraction conditions had further increased the total phenolic content and antioxidant capacity of defatted dabai peel, but not in the pulp. Therefore, optimization of different extraction methods for the defatted fruit parts is recommended for future studies. Penerbit Universiti Kebangsaan Malaysia 2013-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29756/1/Response%20surface%20methodology%20optimization%20for%20extraction%20of%20phenolics%20and%20antioxidant%20capacity%20in%20defatted%20dabai%20parts.pdf Khoo, Hock Eng and Azlan, Azrina and Ismail, Amin and Abas, Faridah (2013) Response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts. Sains Malaysiana, 42 (7). pp. 949-954. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol42num7_2013/vol42num7_2013pg949-954.html
spellingShingle Khoo, Hock Eng
Azlan, Azrina
Ismail, Amin
Abas, Faridah
Response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts
title Response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts
title_full Response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts
title_fullStr Response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts
title_full_unstemmed Response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts
title_short Response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts
title_sort response surface methodology optimization for extraction of phenolics and antioxidant capacity in defatted dabai parts
url http://psasir.upm.edu.my/id/eprint/29756/
http://psasir.upm.edu.my/id/eprint/29756/
http://psasir.upm.edu.my/id/eprint/29756/1/Response%20surface%20methodology%20optimization%20for%20extraction%20of%20phenolics%20and%20antioxidant%20capacity%20in%20defatted%20dabai%20parts.pdf