HbA2 levels in β-thalassaemia carriers with the Filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia?
AIMS: Classical carriers of β-thalassaemia are identified by a raised HbA2 level. Earlier studies indicated that the Filipino β-deletion has high raised HbA2 levels. The introduction of automated high performance liquid chromatography (HPLC) for thalassaemia screening is an important advance in tech...
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| Format: | Article |
| Language: | English |
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Royal College of Pathologists of Australasia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29671/ http://psasir.upm.edu.my/id/eprint/29671/1/HbA2%20levels%20in%20%CE%B2.pdf |
| _version_ | 1848846463764791296 |
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| author | George, Elizabeth Teh, Lai Kuan Tan, Mary Anne Jin Ai Lai, Mei I. Wong, Lily |
| author_facet | George, Elizabeth Teh, Lai Kuan Tan, Mary Anne Jin Ai Lai, Mei I. Wong, Lily |
| author_sort | George, Elizabeth |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | AIMS: Classical carriers of β-thalassaemia are identified by a raised HbA2 level. Earlier studies indicated that the Filipino β-deletion has high raised HbA2 levels. The introduction of automated high performance liquid chromatography (HPLC) for thalassaemia screening is an important advance in technology for haematology laboratories. The BioRad Variant II Hb analyser is a common instrument used to quantify HbA2 levels in thalassaemia screening. This study aimed to determine HbA2 levels in carriers of Filipino β-mutation using the BioRad Variant II Hb analyser.
METHODS: The Filipino β-deletion was identified using gap-polymerase chain reaction (PCR) in the parents of transfusion dependent β-thalassaemia patients who were homozygous for the Filipino β-deletion in the indigenous population of Sabah, Malaysia. Hb subtypes were quantified on the BioRad Variant II Hb analyser. Concurrent α-thalassaemia was identified by multiplex gap-PCR for deletions and amplification refractory mutation system (ARMS)-PCR for non-deletional mutations.
RESULTS: The mean HbA2 level for Filipino β-thalassaemia trait was 5.9 ± 0.47 and with coinheritance of α-thalassaemia was 6.3 ± 0.44 (-α heterozygous) and 6.7 ± 0.36 (-α homozygous). The HbA2 levels were all >4% in keeping with the findings of classical β-thalassaemia trait and significantly higher than levels seen in non-deletional forms of β-thalassaemia.
CONCLUSION: The HbA2 level measured on the BioRad Variant II Hb analyser was lower than the level in the first description of the Filipino β-thalassaemia. β-thalassaemia trait with coinheritance of α-thalassaemia (-α) is associated with significantly higher HbA2 level. |
| first_indexed | 2025-11-15T09:03:07Z |
| format | Article |
| id | upm-29671 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:03:07Z |
| publishDate | 2013 |
| publisher | Royal College of Pathologists of Australasia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-296712016-11-30T02:54:15Z http://psasir.upm.edu.my/id/eprint/29671/ HbA2 levels in β-thalassaemia carriers with the Filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia? George, Elizabeth Teh, Lai Kuan Tan, Mary Anne Jin Ai Lai, Mei I. Wong, Lily AIMS: Classical carriers of β-thalassaemia are identified by a raised HbA2 level. Earlier studies indicated that the Filipino β-deletion has high raised HbA2 levels. The introduction of automated high performance liquid chromatography (HPLC) for thalassaemia screening is an important advance in technology for haematology laboratories. The BioRad Variant II Hb analyser is a common instrument used to quantify HbA2 levels in thalassaemia screening. This study aimed to determine HbA2 levels in carriers of Filipino β-mutation using the BioRad Variant II Hb analyser. METHODS: The Filipino β-deletion was identified using gap-polymerase chain reaction (PCR) in the parents of transfusion dependent β-thalassaemia patients who were homozygous for the Filipino β-deletion in the indigenous population of Sabah, Malaysia. Hb subtypes were quantified on the BioRad Variant II Hb analyser. Concurrent α-thalassaemia was identified by multiplex gap-PCR for deletions and amplification refractory mutation system (ARMS)-PCR for non-deletional mutations. RESULTS: The mean HbA2 level for Filipino β-thalassaemia trait was 5.9 ± 0.47 and with coinheritance of α-thalassaemia was 6.3 ± 0.44 (-α heterozygous) and 6.7 ± 0.36 (-α homozygous). The HbA2 levels were all >4% in keeping with the findings of classical β-thalassaemia trait and significantly higher than levels seen in non-deletional forms of β-thalassaemia. CONCLUSION: The HbA2 level measured on the BioRad Variant II Hb analyser was lower than the level in the first description of the Filipino β-thalassaemia. β-thalassaemia trait with coinheritance of α-thalassaemia (-α) is associated with significantly higher HbA2 level. Royal College of Pathologists of Australasia 2013-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29671/1/HbA2%20levels%20in%20%CE%B2.pdf George, Elizabeth and Teh, Lai Kuan and Tan, Mary Anne Jin Ai and Lai, Mei I. and Wong, Lily (2013) HbA2 levels in β-thalassaemia carriers with the Filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia? Pathology, 45 (1). pp. 62-65. ISSN 0031-3025; ESSN: 1465-3931 10.1097/PAT.0b013e32835af7c1 |
| spellingShingle | George, Elizabeth Teh, Lai Kuan Tan, Mary Anne Jin Ai Lai, Mei I. Wong, Lily HbA2 levels in β-thalassaemia carriers with the Filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia? |
| title | HbA2 levels in β-thalassaemia carriers with the Filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia? |
| title_full | HbA2 levels in β-thalassaemia carriers with the Filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia? |
| title_fullStr | HbA2 levels in β-thalassaemia carriers with the Filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia? |
| title_full_unstemmed | HbA2 levels in β-thalassaemia carriers with the Filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia? |
| title_short | HbA2 levels in β-thalassaemia carriers with the Filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia? |
| title_sort | hba2 levels in β-thalassaemia carriers with the filipino β0-deletion: are the levels higher than what is found with non-deletional forms of β0-thalassaemia? |
| url | http://psasir.upm.edu.my/id/eprint/29671/ http://psasir.upm.edu.my/id/eprint/29671/ http://psasir.upm.edu.my/id/eprint/29671/1/HbA2%20levels%20in%20%CE%B2.pdf |