Anti-inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts
Objective: The study was carried out to determine and compare the anti-inflammatory activities of ethanol extract of flower and leaf of Hibiscus rosa-sinensis var alba(white Hibiscus) and Hibiscus rosa-sinensis L. (red Hibiscus). Methods: In the anti-inflammatory test, 0.1ml of carrageenan was inj...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Innovare Academic Sciences
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/29667/ http://psasir.upm.edu.my/id/eprint/29667/1/Anti.pdf |
| _version_ | 1848846462642814976 |
|---|---|
| author | Raduan, Siti Zaleha Abdul Aziz, Muhammad Wahizul Haswan Abd Hamid @ Abdul Razak, Roslida Zakaria, Zainul Amiruddin Ahmad, Zuraini Abdullah, Muhammad Nazrul Hakim |
| author_facet | Raduan, Siti Zaleha Abdul Aziz, Muhammad Wahizul Haswan Abd Hamid @ Abdul Razak, Roslida Zakaria, Zainul Amiruddin Ahmad, Zuraini Abdullah, Muhammad Nazrul Hakim |
| author_sort | Raduan, Siti Zaleha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: The study was carried out to determine and compare the anti-inflammatory activities of ethanol extract of flower and leaf of Hibiscus rosa-sinensis var alba(white
Hibiscus) and Hibiscus rosa-sinensis L. (red Hibiscus).
Methods: In the anti-inflammatory test, 0.1ml of carrageenan was injected sub plantarly 30 min before administration of each extracts (5, 50 or 100mg/kg). The animals were killed 6 hrs after carrageenan injection and polymorphonuclear infiltration (PNL) in paw tissues were counted. Phytochemical screening was also performed. Acute dose response was determined using Fixed Dose Procedure with fixed level of doses.
Results: The results revealed flavanoids, saponins and steroids presence in all extracts. Dosing of animals up to
500 mg/kg of all extracts caused no toxicity. No significant changes (p>0.05) in liver enzyme levels and histologically no lesions in the organs. Dosing of 50 and 100 mg/kg of flower and leaf estracts of Hibiscus rosa sinensis L. caused significant inhibition (p<0.05) of edema. Flower and leaf of Hibiscus rosa-sinensis var alba
significantly inhibited (p<0.05) edema in all range of testing dose. The white hibiscus revealed a more potent anti
-inflammation. All extracts at various concentration cause
d significant reducti on (p<0.05) on PNL infiltration with white Hibiscus also more potent than red hibiscus. All extracts M showed significant reduction (p<0.05) on the duration of licking response. Same pattern was also observed as white Hibiscus was more potent inhibitor.
Conclusion: This study showed flower and leaf of Hibiscus rosa-sinensis var alba and Hibiscus rosa sinensis L. produced anti-acute inflammatory activity. It may involve the inhibition of cyclooxygenase and reduce PNL and the white Hibiscus variety was more potent than the red variety. |
| first_indexed | 2025-11-15T09:03:06Z |
| format | Article |
| id | upm-29667 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:03:06Z |
| publishDate | 2013 |
| publisher | Innovare Academic Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-296672016-08-23T08:56:23Z http://psasir.upm.edu.my/id/eprint/29667/ Anti-inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts Raduan, Siti Zaleha Abdul Aziz, Muhammad Wahizul Haswan Abd Hamid @ Abdul Razak, Roslida Zakaria, Zainul Amiruddin Ahmad, Zuraini Abdullah, Muhammad Nazrul Hakim Objective: The study was carried out to determine and compare the anti-inflammatory activities of ethanol extract of flower and leaf of Hibiscus rosa-sinensis var alba(white Hibiscus) and Hibiscus rosa-sinensis L. (red Hibiscus). Methods: In the anti-inflammatory test, 0.1ml of carrageenan was injected sub plantarly 30 min before administration of each extracts (5, 50 or 100mg/kg). The animals were killed 6 hrs after carrageenan injection and polymorphonuclear infiltration (PNL) in paw tissues were counted. Phytochemical screening was also performed. Acute dose response was determined using Fixed Dose Procedure with fixed level of doses. Results: The results revealed flavanoids, saponins and steroids presence in all extracts. Dosing of animals up to 500 mg/kg of all extracts caused no toxicity. No significant changes (p>0.05) in liver enzyme levels and histologically no lesions in the organs. Dosing of 50 and 100 mg/kg of flower and leaf estracts of Hibiscus rosa sinensis L. caused significant inhibition (p<0.05) of edema. Flower and leaf of Hibiscus rosa-sinensis var alba significantly inhibited (p<0.05) edema in all range of testing dose. The white hibiscus revealed a more potent anti -inflammation. All extracts at various concentration cause d significant reducti on (p<0.05) on PNL infiltration with white Hibiscus also more potent than red hibiscus. All extracts M showed significant reduction (p<0.05) on the duration of licking response. Same pattern was also observed as white Hibiscus was more potent inhibitor. Conclusion: This study showed flower and leaf of Hibiscus rosa-sinensis var alba and Hibiscus rosa sinensis L. produced anti-acute inflammatory activity. It may involve the inhibition of cyclooxygenase and reduce PNL and the white Hibiscus variety was more potent than the red variety. Innovare Academic Sciences 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29667/1/Anti.pdf Raduan, Siti Zaleha and Abdul Aziz, Muhammad Wahizul Haswan and Abd Hamid @ Abdul Razak, Roslida and Zakaria, Zainul Amiruddin and Ahmad, Zuraini and Abdullah, Muhammad Nazrul Hakim (2013) Anti-inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts. International Journal of Pharmacy and Pharmaceutical Sciences, 5 (4). pp. 754-762. ISSN 0975-1491 http://www.ijppsjournal.com/Vol5Issue4.htm |
| spellingShingle | Raduan, Siti Zaleha Abdul Aziz, Muhammad Wahizul Haswan Abd Hamid @ Abdul Razak, Roslida Zakaria, Zainul Amiruddin Ahmad, Zuraini Abdullah, Muhammad Nazrul Hakim Anti-inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts |
| title | Anti-inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts |
| title_full | Anti-inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts |
| title_fullStr | Anti-inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts |
| title_full_unstemmed | Anti-inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts |
| title_short | Anti-inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts |
| title_sort | anti-inflammatory effects of hibiscus rosa-sinensis l. and hibiscus rosa-sinensis var. alba ethanol extracts |
| url | http://psasir.upm.edu.my/id/eprint/29667/ http://psasir.upm.edu.my/id/eprint/29667/ http://psasir.upm.edu.my/id/eprint/29667/1/Anti.pdf |