Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum birivilianum
The present paper focused on antioxidant and cytotoxicity assessment of crude and total saponin fraction of Chlorophytum borivilianum as an important medicinal plant. In this study, three different antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), ferrous ion chelating...
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| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29631/ http://psasir.upm.edu.my/id/eprint/29631/1/Assessment%20of%20antioxidant%20and%20cytotoxicity%20activities%20of%20saponin%20and%20crude%20extracts%20of%20Chlorophytum%20birivilianum.pdf |
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| author | Ashraf, Mehdi Farshad Abdul Aziz, Maheran Stanslas, Johnson Ismail, Ismanizam Abdul Kadir, Mihdzar |
| author_facet | Ashraf, Mehdi Farshad Abdul Aziz, Maheran Stanslas, Johnson Ismail, Ismanizam Abdul Kadir, Mihdzar |
| author_sort | Ashraf, Mehdi Farshad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present paper focused on antioxidant and cytotoxicity assessment of crude and total saponin fraction of Chlorophytum borivilianum as an important medicinal plant. In this study, three different antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), ferrous ion chelating (FIC), and β-carotene bleaching (BCB) activity) of crude extract and total saponin fraction of C. borivilianum tubers were performed. Crude extract was found to possess higher free radical scavenging activity (ascorbic acid equivalents 2578 ± 111 mg AA/100 g) and bleaching activity (IC50 = 0.7 mg mL−1), while total saponin fraction displayed higher ferrous ion chelating (EC50 = 1 mg mL−1). Cytotoxicity evaluation of crude extract and total saponin fraction against MCF-7, PC3, and HCT-116 cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) cell viability assay indicated a higher cytotoxicity activity of the crude extract than the total saponin fraction on all cell lines, being most effective and selective on MCF-7 human breast cancer cell line. |
| first_indexed | 2025-11-15T09:02:56Z |
| format | Article |
| id | upm-29631 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:56Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-296312017-04-26T09:19:12Z http://psasir.upm.edu.my/id/eprint/29631/ Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum birivilianum Ashraf, Mehdi Farshad Abdul Aziz, Maheran Stanslas, Johnson Ismail, Ismanizam Abdul Kadir, Mihdzar The present paper focused on antioxidant and cytotoxicity assessment of crude and total saponin fraction of Chlorophytum borivilianum as an important medicinal plant. In this study, three different antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), ferrous ion chelating (FIC), and β-carotene bleaching (BCB) activity) of crude extract and total saponin fraction of C. borivilianum tubers were performed. Crude extract was found to possess higher free radical scavenging activity (ascorbic acid equivalents 2578 ± 111 mg AA/100 g) and bleaching activity (IC50 = 0.7 mg mL−1), while total saponin fraction displayed higher ferrous ion chelating (EC50 = 1 mg mL−1). Cytotoxicity evaluation of crude extract and total saponin fraction against MCF-7, PC3, and HCT-116 cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) cell viability assay indicated a higher cytotoxicity activity of the crude extract than the total saponin fraction on all cell lines, being most effective and selective on MCF-7 human breast cancer cell line. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29631/1/Assessment%20of%20antioxidant%20and%20cytotoxicity%20activities%20of%20saponin%20and%20crude%20extracts%20of%20Chlorophytum%20birivilianum.pdf Ashraf, Mehdi Farshad and Abdul Aziz, Maheran and Stanslas, Johnson and Ismail, Ismanizam and Abdul Kadir, Mihdzar (2013) Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum birivilianum. The Scientific World Journal, 2013. art. no. 216894. pp. 1-7. ISSN 2356-6140; ESSN: 1537-744X https://www.hindawi.com/journals/tswj/2013/216894/abs/ 10.1155/2013/216894 |
| spellingShingle | Ashraf, Mehdi Farshad Abdul Aziz, Maheran Stanslas, Johnson Ismail, Ismanizam Abdul Kadir, Mihdzar Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum birivilianum |
| title | Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum birivilianum |
| title_full | Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum birivilianum |
| title_fullStr | Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum birivilianum |
| title_full_unstemmed | Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum birivilianum |
| title_short | Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of Chlorophytum birivilianum |
| title_sort | assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of chlorophytum birivilianum |
| url | http://psasir.upm.edu.my/id/eprint/29631/ http://psasir.upm.edu.my/id/eprint/29631/ http://psasir.upm.edu.my/id/eprint/29631/ http://psasir.upm.edu.my/id/eprint/29631/1/Assessment%20of%20antioxidant%20and%20cytotoxicity%20activities%20of%20saponin%20and%20crude%20extracts%20of%20Chlorophytum%20birivilianum.pdf |