Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients
Background: Somatic mutations of the epidermal growth factor receptor (EGFR) are reportedly associated with various responses in non-small cell lung cancer (NSCLC) patients receiving the anti-EGFR agents. Detection of the mutation therefore plays an important role in therapeutic decision making. The...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29589/ http://psasir.upm.edu.my/id/eprint/29589/1/Detection%20of%20epidermal%20growth%20factor%20receptor%20mutations%20in%20formalin%20fixed%20paraffin%20embedded%20biopsies%20in%20Malaysian%20non.pdf |
| _version_ | 1848846441072558080 |
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| author | Ng, Tiffany Shi Yeen Pathmanathan, Rajadurai Mohd Sidik, Shiran Azman, Ahmad Zaid Fattah Cheah, Yoke Kqueen |
| author_facet | Ng, Tiffany Shi Yeen Pathmanathan, Rajadurai Mohd Sidik, Shiran Azman, Ahmad Zaid Fattah Cheah, Yoke Kqueen |
| author_sort | Ng, Tiffany Shi Yeen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Somatic mutations of the epidermal growth factor receptor (EGFR) are reportedly associated with various responses in non-small cell lung cancer (NSCLC) patients receiving the anti-EGFR agents. Detection of the mutation therefore plays an important role in therapeutic decision making. The aim of this study was to detect EGFR mutations in formalin fixed paraffin embedded (FFPE) samples using both Scorpion ARMS and high resolution melt (HRM) assay, and to compare the sensitivity of these methods.
Results: All of the mutations were found in adenocarcinoma, except one that was in squamous cell carcinoma. The mutation rate was 45.7% (221/484). Complex mutations were also observed, wherein 8 tumours carried 2 mutations and 1 tumour carried 3 mutations.
Conclusions: Both methods detected EGFR mutations in FFPE samples. HRM assays gave more EGFR positive results compared to Scorpion ARMS. |
| first_indexed | 2025-11-15T09:02:45Z |
| format | Article |
| id | upm-29589 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:45Z |
| publishDate | 2013 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-295892015-12-07T08:38:39Z http://psasir.upm.edu.my/id/eprint/29589/ Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients Ng, Tiffany Shi Yeen Pathmanathan, Rajadurai Mohd Sidik, Shiran Azman, Ahmad Zaid Fattah Cheah, Yoke Kqueen Background: Somatic mutations of the epidermal growth factor receptor (EGFR) are reportedly associated with various responses in non-small cell lung cancer (NSCLC) patients receiving the anti-EGFR agents. Detection of the mutation therefore plays an important role in therapeutic decision making. The aim of this study was to detect EGFR mutations in formalin fixed paraffin embedded (FFPE) samples using both Scorpion ARMS and high resolution melt (HRM) assay, and to compare the sensitivity of these methods. Results: All of the mutations were found in adenocarcinoma, except one that was in squamous cell carcinoma. The mutation rate was 45.7% (221/484). Complex mutations were also observed, wherein 8 tumours carried 2 mutations and 1 tumour carried 3 mutations. Conclusions: Both methods detected EGFR mutations in FFPE samples. HRM assays gave more EGFR positive results compared to Scorpion ARMS. BioMed Central 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29589/1/Detection%20of%20epidermal%20growth%20factor%20receptor%20mutations%20in%20formalin%20fixed%20paraffin%20embedded%20biopsies%20in%20Malaysian%20non.pdf Ng, Tiffany Shi Yeen and Pathmanathan, Rajadurai and Mohd Sidik, Shiran and Azman, Ahmad Zaid Fattah and Cheah, Yoke Kqueen (2013) Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients. Journal of Biomedical Science, 20. art. no. 22. pp. 1-7. ISSN 1021-7770; ESSN: 1423-0127 http://www.jbiomedsci.com/content/20/1/22 10.1186/1423-0127-20-22 |
| spellingShingle | Ng, Tiffany Shi Yeen Pathmanathan, Rajadurai Mohd Sidik, Shiran Azman, Ahmad Zaid Fattah Cheah, Yoke Kqueen Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients |
| title | Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients |
| title_full | Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients |
| title_fullStr | Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients |
| title_full_unstemmed | Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients |
| title_short | Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients |
| title_sort | detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in malaysian non-small cell lung cancer patients |
| url | http://psasir.upm.edu.my/id/eprint/29589/ http://psasir.upm.edu.my/id/eprint/29589/ http://psasir.upm.edu.my/id/eprint/29589/ http://psasir.upm.edu.my/id/eprint/29589/1/Detection%20of%20epidermal%20growth%20factor%20receptor%20mutations%20in%20formalin%20fixed%20paraffin%20embedded%20biopsies%20in%20Malaysian%20non.pdf |