Study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in Sprague Dawley rats

Toxicological studies constitute an essential part of the effort in developing an herbal medicine into a drug product. A newly developed thymoquinone-rich fraction nanoemulsion (TQRFNE) has been prepared using a high pressure homogenizer. The purpose of this study was to investigate the potential ac...

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Main Authors: Tubesha, Zaki, Imam, Mustapha Umar, Mahmud, Rozi, Ismail, Maznah
Format: Article
Language:English
Published: MDPI 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29585/
http://psasir.upm.edu.my/id/eprint/29585/1/Study%20on%20the%20potential%20toxicity%20of%20a%20thymoquinone.pdf
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author Tubesha, Zaki
Imam, Mustapha Umar
Mahmud, Rozi
Ismail, Maznah
author_facet Tubesha, Zaki
Imam, Mustapha Umar
Mahmud, Rozi
Ismail, Maznah
author_sort Tubesha, Zaki
building UPM Institutional Repository
collection Online Access
description Toxicological studies constitute an essential part of the effort in developing an herbal medicine into a drug product. A newly developed thymoquinone-rich fraction nanoemulsion (TQRFNE) has been prepared using a high pressure homogenizer. The purpose of this study was to investigate the potential acute toxicity of this nanoemulsion in Sprague Dawley rats. The acute toxicity studies were conducted as per the OECD guidelines 425, allowing for the use of test dose limit of 20 mL TQRFNE (containing 44.5 mg TQ)/kg. TQRFNE and distilled water (DW) as a control were administered orally to both sexes of rats on Day 0 and observed for 14 days. All the animals appeared normal, and healthy throughout the study. There was no observed mortality or any signs of toxicity during the experimental period. The effects of the TQRFNE and DW groups on general behavior, body weight, food and water consumption, relative organ weight, hematology, histopathology, and clinical biochemistry were measured. All the parameters measured were unaffected as compared to the control (DW) group. The administration of 20 mL TQRFNE /kg was not toxic after an acute exposure.
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spelling upm-295852015-12-29T02:37:50Z http://psasir.upm.edu.my/id/eprint/29585/ Study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in Sprague Dawley rats Tubesha, Zaki Imam, Mustapha Umar Mahmud, Rozi Ismail, Maznah Toxicological studies constitute an essential part of the effort in developing an herbal medicine into a drug product. A newly developed thymoquinone-rich fraction nanoemulsion (TQRFNE) has been prepared using a high pressure homogenizer. The purpose of this study was to investigate the potential acute toxicity of this nanoemulsion in Sprague Dawley rats. The acute toxicity studies were conducted as per the OECD guidelines 425, allowing for the use of test dose limit of 20 mL TQRFNE (containing 44.5 mg TQ)/kg. TQRFNE and distilled water (DW) as a control were administered orally to both sexes of rats on Day 0 and observed for 14 days. All the animals appeared normal, and healthy throughout the study. There was no observed mortality or any signs of toxicity during the experimental period. The effects of the TQRFNE and DW groups on general behavior, body weight, food and water consumption, relative organ weight, hematology, histopathology, and clinical biochemistry were measured. All the parameters measured were unaffected as compared to the control (DW) group. The administration of 20 mL TQRFNE /kg was not toxic after an acute exposure. MDPI 2013-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29585/1/Study%20on%20the%20potential%20toxicity%20of%20a%20thymoquinone.pdf Tubesha, Zaki and Imam, Mustapha Umar and Mahmud, Rozi and Ismail, Maznah (2013) Study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in Sprague Dawley rats. Molecules, 18 (7). pp. 7460-7472. ISSN 1420-3049 http://www.mdpi.com/1420-3049/18/7/7460 10.3390/molecules18077460
spellingShingle Tubesha, Zaki
Imam, Mustapha Umar
Mahmud, Rozi
Ismail, Maznah
Study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in Sprague Dawley rats
title Study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in Sprague Dawley rats
title_full Study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in Sprague Dawley rats
title_fullStr Study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in Sprague Dawley rats
title_full_unstemmed Study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in Sprague Dawley rats
title_short Study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in Sprague Dawley rats
title_sort study on the potential toxicity of a thymoquinone-rich fraction nanoemulsion in sprague dawley rats
url http://psasir.upm.edu.my/id/eprint/29585/
http://psasir.upm.edu.my/id/eprint/29585/
http://psasir.upm.edu.my/id/eprint/29585/
http://psasir.upm.edu.my/id/eprint/29585/1/Study%20on%20the%20potential%20toxicity%20of%20a%20thymoquinone.pdf