Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction
Amplified chitinase gene sequence shared 99% homology with Musa acuminata class III acidic chitinase and beta-1,3-glucanase gene sequence was 100% homologous to Musa x paradisiaca beta-1,3-glucanase. Three nucleotide-binding sites and the leucine-rich repeat (NBS-LRR) type of putative RGCs and one s...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29584/ http://psasir.upm.edu.my/id/eprint/29584/1/05%20JTAS-0684-2014.pdf |
| _version_ | 1848846439772323840 |
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| author | Yang, Chee Yong Hamid, Sathyapriya Wong, Mui Yun |
| author_facet | Yang, Chee Yong Hamid, Sathyapriya Wong, Mui Yun |
| author_sort | Yang, Chee Yong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Amplified chitinase gene sequence shared 99% homology with Musa acuminata class III acidic chitinase and beta-1,3-glucanase gene sequence was 100% homologous to Musa x paradisiaca beta-1,3-glucanase. Three nucleotide-binding sites and the leucine-rich repeat (NBS-LRR) type of putative RGCs and one serine/threonine kinase gene were characterised at the amino acid level. Kinase-2 (LVLDDVW) and kinase-3 (GSRIIITTRD) motifs in the nucleotide-binding domain were highly conserved in RGC2 and RGC3 and these genes belong to the non-TIR-NBS class RGCs. RGC1 was also clustered into non-TIR-NBS class RGCs; however, many residue substitutions were present in the kinase-2 and kinase-3 motifs. The sub-domain IX (LTEKSDVYSFGVVL) of serine/threonine protein kinase was highly conserved in RGC5 and it shared highest homology with PTH-2 from muskmelon. RT-PCR analysis revealed the differential expression of PR and RGC genes exhibited by different banana genotypes over sampling time. Chitinase was expressed during banana- FocR4 interaction in all three banana genotypes. However, its expression was high and constant in Rastali Mutiara during banana-FocR4 interaction and resulted in very low disease severity in FocR4 inoculated plants (2%) compared to Rastali wild-type (16%) and Jari Buaya (8%) at six weeks after inoculation. This suggests that chitinase may play an important role in disease resistance against FocR4. Besides, our study also shows that Rastali Mutiara can be a potential source of disease-resistant genes for molecular breeding of banana. |
| first_indexed | 2025-11-15T09:02:44Z |
| format | Article |
| id | upm-29584 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:44Z |
| publishDate | 2016 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-295842016-07-12T03:52:17Z http://psasir.upm.edu.my/id/eprint/29584/ Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction Yang, Chee Yong Hamid, Sathyapriya Wong, Mui Yun Amplified chitinase gene sequence shared 99% homology with Musa acuminata class III acidic chitinase and beta-1,3-glucanase gene sequence was 100% homologous to Musa x paradisiaca beta-1,3-glucanase. Three nucleotide-binding sites and the leucine-rich repeat (NBS-LRR) type of putative RGCs and one serine/threonine kinase gene were characterised at the amino acid level. Kinase-2 (LVLDDVW) and kinase-3 (GSRIIITTRD) motifs in the nucleotide-binding domain were highly conserved in RGC2 and RGC3 and these genes belong to the non-TIR-NBS class RGCs. RGC1 was also clustered into non-TIR-NBS class RGCs; however, many residue substitutions were present in the kinase-2 and kinase-3 motifs. The sub-domain IX (LTEKSDVYSFGVVL) of serine/threonine protein kinase was highly conserved in RGC5 and it shared highest homology with PTH-2 from muskmelon. RT-PCR analysis revealed the differential expression of PR and RGC genes exhibited by different banana genotypes over sampling time. Chitinase was expressed during banana- FocR4 interaction in all three banana genotypes. However, its expression was high and constant in Rastali Mutiara during banana-FocR4 interaction and resulted in very low disease severity in FocR4 inoculated plants (2%) compared to Rastali wild-type (16%) and Jari Buaya (8%) at six weeks after inoculation. This suggests that chitinase may play an important role in disease resistance against FocR4. Besides, our study also shows that Rastali Mutiara can be a potential source of disease-resistant genes for molecular breeding of banana. Universiti Putra Malaysia Press 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29584/1/05%20JTAS-0684-2014.pdf Yang, Chee Yong and Hamid, Sathyapriya and Wong, Mui Yun (2016) Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction. Pertanika Journal of Tropical Agricultural Science, 39 (1). pp. 55-72. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2039%20(1)%20Feb.%202016/05%20JTAS-0684-2014.pdf |
| spellingShingle | Yang, Chee Yong Hamid, Sathyapriya Wong, Mui Yun Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction |
| title | Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction |
| title_full | Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction |
| title_fullStr | Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction |
| title_full_unstemmed | Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction |
| title_short | Characterisation of pathogenesis-related genes and resistance gene candidates in banana (Musa acuminata) and their expression during host-pathogen interaction |
| title_sort | characterisation of pathogenesis-related genes and resistance gene candidates in banana (musa acuminata) and their expression during host-pathogen interaction |
| url | http://psasir.upm.edu.my/id/eprint/29584/ http://psasir.upm.edu.my/id/eprint/29584/ http://psasir.upm.edu.my/id/eprint/29584/1/05%20JTAS-0684-2014.pdf |