Hepatoprotective activity of methanolic extract of Bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats
In an attempt to further establish the pharmacological properties of Bauhinia purpurea (Fabaceae), hepatoprotective potential of methanol extract of B. purpurea leaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats () were used and administe...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/29571/ http://psasir.upm.edu.my/id/eprint/29571/1/Hepatoprotective%20Activity%20of%20Methanolic%20Extract%20of%20Bauhinia%20purpurea%20Leaves%20against%20Paracetamol-Induced%20Hepatic%20Damage%20in%20Rats.pdf |
| _version_ | 1848846436261691392 |
|---|---|
| author | Yahya, Farhana Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Seyyedan, Atefeh Alsadat Jakius, Krystal Feredoline Mahmood, Nur Diyana Shahril, Mohamad Syahmi Suhaili, Zarizal Mohtarrudin, Norhafizah Deny Susanti, Somchit, Muhammad Nazrul Teh, Lay Kek Salleh, Mohd Zaki Zakaria, Zainul Amiruddin |
| author_facet | Yahya, Farhana Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Seyyedan, Atefeh Alsadat Jakius, Krystal Feredoline Mahmood, Nur Diyana Shahril, Mohamad Syahmi Suhaili, Zarizal Mohtarrudin, Norhafizah Deny Susanti, Somchit, Muhammad Nazrul Teh, Lay Kek Salleh, Mohd Zaki Zakaria, Zainul Amiruddin |
| author_sort | Yahya, Farhana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In an attempt to further establish the pharmacological properties of Bauhinia purpurea (Fabaceae), hepatoprotective potential of methanol extract of B. purpurea leaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats () were used and administered orally once daily with 10% DMSO (negative control), 200 mg/kg silymarin (positive control), or MEBP (50, 250, and 500 mg/kg) for 7 days, followed by the hepatotoxicity induction using paracetamol (PCM). The blood samples and livers were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay with the total phenolic content (TPC) also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of the normal hepatic structural was observed in group pretreated with silymarin and MEBP. Hepatotoxic rats pretreated with silymarin or MEBP exhibited significant decrease () in ALT and AST enzyme level. Moreover, the extract also exhibited antioxidant activity and contained high TPC. In conclusion, MEBP exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and high phenolic content and thus warrants further investigation. |
| first_indexed | 2025-11-15T09:02:41Z |
| format | Article |
| id | upm-29571 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:41Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-295712016-01-18T05:28:54Z http://psasir.upm.edu.my/id/eprint/29571/ Hepatoprotective activity of methanolic extract of Bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats Yahya, Farhana Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Seyyedan, Atefeh Alsadat Jakius, Krystal Feredoline Mahmood, Nur Diyana Shahril, Mohamad Syahmi Suhaili, Zarizal Mohtarrudin, Norhafizah Deny Susanti, Somchit, Muhammad Nazrul Teh, Lay Kek Salleh, Mohd Zaki Zakaria, Zainul Amiruddin In an attempt to further establish the pharmacological properties of Bauhinia purpurea (Fabaceae), hepatoprotective potential of methanol extract of B. purpurea leaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats () were used and administered orally once daily with 10% DMSO (negative control), 200 mg/kg silymarin (positive control), or MEBP (50, 250, and 500 mg/kg) for 7 days, followed by the hepatotoxicity induction using paracetamol (PCM). The blood samples and livers were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay with the total phenolic content (TPC) also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of the normal hepatic structural was observed in group pretreated with silymarin and MEBP. Hepatotoxic rats pretreated with silymarin or MEBP exhibited significant decrease () in ALT and AST enzyme level. Moreover, the extract also exhibited antioxidant activity and contained high TPC. In conclusion, MEBP exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and high phenolic content and thus warrants further investigation. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29571/1/Hepatoprotective%20Activity%20of%20Methanolic%20Extract%20of%20Bauhinia%20purpurea%20Leaves%20against%20Paracetamol-Induced%20Hepatic%20Damage%20in%20Rats.pdf Yahya, Farhana and Mamat, Siti Syariah and Kamarolzaman, Mohammad Fauzi Fahmi and Seyyedan, Atefeh Alsadat and Jakius, Krystal Feredoline and Mahmood, Nur Diyana and Shahril, Mohamad Syahmi and Suhaili, Zarizal and Mohtarrudin, Norhafizah and Deny Susanti, and Somchit, Muhammad Nazrul and Teh, Lay Kek and Salleh, Mohd Zaki and Zakaria, Zainul Amiruddin (2013) Hepatoprotective activity of methanolic extract of Bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats. Evidence-Based Complementary and Alternative Medicine, 2013. art. no. 636580. pp. 1-10. ISSN 1741-427X; ESSN: 1741-4288 http://www.hindawi.com/journals/ecam/2013/636580/abs/ 10.1155/2013/636580 |
| spellingShingle | Yahya, Farhana Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Seyyedan, Atefeh Alsadat Jakius, Krystal Feredoline Mahmood, Nur Diyana Shahril, Mohamad Syahmi Suhaili, Zarizal Mohtarrudin, Norhafizah Deny Susanti, Somchit, Muhammad Nazrul Teh, Lay Kek Salleh, Mohd Zaki Zakaria, Zainul Amiruddin Hepatoprotective activity of methanolic extract of Bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats |
| title | Hepatoprotective activity of methanolic extract of Bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats |
| title_full | Hepatoprotective activity of methanolic extract of Bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats |
| title_fullStr | Hepatoprotective activity of methanolic extract of Bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats |
| title_full_unstemmed | Hepatoprotective activity of methanolic extract of Bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats |
| title_short | Hepatoprotective activity of methanolic extract of Bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats |
| title_sort | hepatoprotective activity of methanolic extract of bauhinia purpurea leaves against paracetamol-induced hepatic damage in rats |
| url | http://psasir.upm.edu.my/id/eprint/29571/ http://psasir.upm.edu.my/id/eprint/29571/ http://psasir.upm.edu.my/id/eprint/29571/ http://psasir.upm.edu.my/id/eprint/29571/1/Hepatoprotective%20Activity%20of%20Methanolic%20Extract%20of%20Bauhinia%20purpurea%20Leaves%20against%20Paracetamol-Induced%20Hepatic%20Damage%20in%20Rats.pdf |