High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia
Little is known on the genetic relatedness and potential dissemination of particular enterococcal clones in Malaysia. We studied the antibiotic susceptibility profiles of Enterococcus faecium and Enterococcus faecalis and subjected them to pulsed-field gel electrophoresis (PFGE) and multilocus seque...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29560/ http://psasir.upm.edu.my/id/eprint/29560/1/High%20Genetic%20Diversity%20of%20Enterococcus%20faecium%20and%20Enterococcus%20faecalis%20Clinical%20Isolates%20by%20Pulsed-Field%20Gel%20Electrophoresis%20and%20Multilocus%20Sequence%20Typing%20from%20a%20Hospital%20in%20Malaysia.pdf |
| _version_ | 1848846433163149312 |
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| author | Weng, Poh Leng Ramli, Ramliza Shamsudin, Mariana Nor Cheah, Yoke Kqueen Awang Hamat, Rukman |
| author_facet | Weng, Poh Leng Ramli, Ramliza Shamsudin, Mariana Nor Cheah, Yoke Kqueen Awang Hamat, Rukman |
| author_sort | Weng, Poh Leng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Little is known on the genetic relatedness and potential dissemination of particular enterococcal clones in Malaysia. We studied the antibiotic susceptibility profiles of Enterococcus faecium and Enterococcus faecalis and subjected them to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). E. faecium and E. faecalis displayed 27 and 30 pulsotypes, respectively, and 10 representative E. faecium and E. faecalis isolates (five each) yielded few different sequence types (STs): ST17 (2 isolates), ST78, ST203, and ST601 for E. faecium, and ST6, ST16, ST28, ST179, and ST399 for E. faecalis. Resistance to tazobactam-piperacillin and ampicillin amongst E. faecium isolates was highly observed as compared to E. faecalis isolates. All of the isolates were sensitive to vancomycin and teicoplanin. The presence of epidemic and nosocomial strains of selected E. faecium STs: 17, 78, and 203 and E. faecalis ST6 as well as high rates of resistance to multiple antibiotics amongst E. faecium isolates is of a particular concern. |
| first_indexed | 2025-11-15T09:02:38Z |
| format | Article |
| id | upm-29560 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:38Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-295602015-12-08T07:16:53Z http://psasir.upm.edu.my/id/eprint/29560/ High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia Weng, Poh Leng Ramli, Ramliza Shamsudin, Mariana Nor Cheah, Yoke Kqueen Awang Hamat, Rukman Little is known on the genetic relatedness and potential dissemination of particular enterococcal clones in Malaysia. We studied the antibiotic susceptibility profiles of Enterococcus faecium and Enterococcus faecalis and subjected them to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). E. faecium and E. faecalis displayed 27 and 30 pulsotypes, respectively, and 10 representative E. faecium and E. faecalis isolates (five each) yielded few different sequence types (STs): ST17 (2 isolates), ST78, ST203, and ST601 for E. faecium, and ST6, ST16, ST28, ST179, and ST399 for E. faecalis. Resistance to tazobactam-piperacillin and ampicillin amongst E. faecium isolates was highly observed as compared to E. faecalis isolates. All of the isolates were sensitive to vancomycin and teicoplanin. The presence of epidemic and nosocomial strains of selected E. faecium STs: 17, 78, and 203 and E. faecalis ST6 as well as high rates of resistance to multiple antibiotics amongst E. faecium isolates is of a particular concern. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29560/1/High%20Genetic%20Diversity%20of%20Enterococcus%20faecium%20and%20Enterococcus%20faecalis%20Clinical%20Isolates%20by%20Pulsed-Field%20Gel%20Electrophoresis%20and%20Multilocus%20Sequence%20Typing%20from%20a%20Hospital%20in%20Malaysia.pdf Weng, Poh Leng and Ramli, Ramliza and Shamsudin, Mariana Nor and Cheah, Yoke Kqueen and Awang Hamat, Rukman (2013) High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia. BioMed Research International, 2013. art. no. 938937. pp. 1-6. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2013/938937/abs/ 10.1155/2013/938937 |
| spellingShingle | Weng, Poh Leng Ramli, Ramliza Shamsudin, Mariana Nor Cheah, Yoke Kqueen Awang Hamat, Rukman High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia |
| title | High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia |
| title_full | High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia |
| title_fullStr | High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia |
| title_full_unstemmed | High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia |
| title_short | High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia |
| title_sort | high genetic diversity of enterococcus faecium and enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/29560/ http://psasir.upm.edu.my/id/eprint/29560/ http://psasir.upm.edu.my/id/eprint/29560/ http://psasir.upm.edu.my/id/eprint/29560/1/High%20Genetic%20Diversity%20of%20Enterococcus%20faecium%20and%20Enterococcus%20faecalis%20Clinical%20Isolates%20by%20Pulsed-Field%20Gel%20Electrophoresis%20and%20Multilocus%20Sequence%20Typing%20from%20a%20Hospital%20in%20Malaysia.pdf |