Branched DNA: a novel technique for molecular diagnostics in bone studies
Recently, there are increasing applications of molecular techniques for molecular diagnostics in bone studies. Bone is a mineralized tissue containing an abundant matrix, which makes RNA isolation difficult. The most common technique used for molecular diagnosis in bone disease is reverse transcript...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine, Universiti Kebangsaan Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29552/ http://psasir.upm.edu.my/id/eprint/29552/1/Branched%20DNA%20a%20novel%20technique%20for%20molecular%20diagnostics%20in%20bone%20studies.pdf |
| _version_ | 1848846430618255360 |
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| author | Ahmad Ariffin, Siti Noor Fathilah |
| author_facet | Ahmad Ariffin, Siti Noor Fathilah |
| author_sort | Ahmad Ariffin, Siti Noor Fathilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Recently, there are increasing applications of molecular techniques for molecular diagnostics in bone studies. Bone is a mineralized tissue containing an abundant matrix, which makes RNA isolation difficult. The most common technique used for molecular diagnosis in bone disease is reverse transcriptase–polymerase chain reaction (RT-PCR) sequence amplification assay. Currently, there is an in-creasing use of the branched chain DNA (bDNA) signal amplification technology. This article provides an overview of bDNA technology for molecular diagnostics in bone studies. Molecular diagnostic assays using bDNA technology for detection of gene expressions are sensitive, specific and reliable tools in the detection of genes involved in bone diseases such as osteoporosis. |
| first_indexed | 2025-11-15T09:02:35Z |
| format | Article |
| id | upm-29552 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:35Z |
| publishDate | 2013 |
| publisher | Faculty of Medicine, Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-295522016-07-12T08:38:00Z http://psasir.upm.edu.my/id/eprint/29552/ Branched DNA: a novel technique for molecular diagnostics in bone studies Ahmad Ariffin, Siti Noor Fathilah Recently, there are increasing applications of molecular techniques for molecular diagnostics in bone studies. Bone is a mineralized tissue containing an abundant matrix, which makes RNA isolation difficult. The most common technique used for molecular diagnosis in bone disease is reverse transcriptase–polymerase chain reaction (RT-PCR) sequence amplification assay. Currently, there is an in-creasing use of the branched chain DNA (bDNA) signal amplification technology. This article provides an overview of bDNA technology for molecular diagnostics in bone studies. Molecular diagnostic assays using bDNA technology for detection of gene expressions are sensitive, specific and reliable tools in the detection of genes involved in bone diseases such as osteoporosis. Faculty of Medicine, Universiti Kebangsaan Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29552/1/Branched%20DNA%20a%20novel%20technique%20for%20molecular%20diagnostics%20in%20bone%20studies.pdf Ahmad Ariffin, Siti Noor Fathilah (2013) Branched DNA: a novel technique for molecular diagnostics in bone studies. Research Updates in Medical Sciences, 1 (1). pp. 27-29. ISSN 2289-2141 http://spaj.ukm.my/fper/index.php/rumes/article/view/27 |
| spellingShingle | Ahmad Ariffin, Siti Noor Fathilah Branched DNA: a novel technique for molecular diagnostics in bone studies |
| title | Branched DNA: a novel technique for molecular diagnostics in bone studies |
| title_full | Branched DNA: a novel technique for molecular diagnostics in bone studies |
| title_fullStr | Branched DNA: a novel technique for molecular diagnostics in bone studies |
| title_full_unstemmed | Branched DNA: a novel technique for molecular diagnostics in bone studies |
| title_short | Branched DNA: a novel technique for molecular diagnostics in bone studies |
| title_sort | branched dna: a novel technique for molecular diagnostics in bone studies |
| url | http://psasir.upm.edu.my/id/eprint/29552/ http://psasir.upm.edu.my/id/eprint/29552/ http://psasir.upm.edu.my/id/eprint/29552/1/Branched%20DNA%20a%20novel%20technique%20for%20molecular%20diagnostics%20in%20bone%20studies.pdf |