Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies
Objective: To quantify circulating fetal DNA (fDNA) levels in the second and third trimesters of normal healthy pregnant individuals and pregnant women with the following clinical conditions: gestational diabetes mellitus (GDM), iron deficiency anemia and gestational hypertension (GHT). Methods: Th...
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| Format: | Article |
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Taylor & Francis
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29547/ |
| _version_ | 1848846429242523648 |
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| author | Zamanpoor, Mansour Rosli, Rozita Yazid, Mohd Nazri Husain, Zaheed Nordin, Norshariza Karuppiah, Thilakavathy |
| author_facet | Zamanpoor, Mansour Rosli, Rozita Yazid, Mohd Nazri Husain, Zaheed Nordin, Norshariza Karuppiah, Thilakavathy |
| author_sort | Zamanpoor, Mansour |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: To quantify circulating fetal DNA (fDNA) levels in the second and third trimesters of normal healthy pregnant individuals and pregnant women with the following clinical conditions: gestational diabetes mellitus (GDM), iron deficiency anemia and gestational hypertension (GHT).
Methods: The SRY gene located on the Y chromosome was used as a unique fetal marker. The fDNA was extracted from maternal plasma and the SRY gene concentrations were measured by quantitative real-time polymerase chain reaction (PCR) amplification using TaqMan dual labeled probe system.
Results: No significant differences were observed in the mean fDNA concentration between normal and GDM pregnancy samples (p > 0.05) and also between normal and anemic pregnancy samples (p > 0.05) in both trimesters, but significant differences were observed between the third trimester normal and GHT pregnancy samples (p = 0.001). GDM and iron deficiency anemia do not affect the levels of fDNA in maternal plasma while GHT significantly elevates the levels of fDNA in maternal plasma.
Conclusions: Increased amount of circulating fDNA in maternal plasma could be used for early identification of adverse pregnancies. GDM and anemia do not affect the levels of fDNA in maternal plasma while GHT significantly elevates the levels of fDNA in maternal plasma. Hence, the elevated fDNA values could be used as a potential screening marker in pregnancies complicated with GHT but not with GDM and iron deficiency anemia. |
| first_indexed | 2025-11-15T09:02:34Z |
| format | Article |
| id | upm-29547 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:02:34Z |
| publishDate | 2013 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-295472015-12-08T04:48:19Z http://psasir.upm.edu.my/id/eprint/29547/ Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies Zamanpoor, Mansour Rosli, Rozita Yazid, Mohd Nazri Husain, Zaheed Nordin, Norshariza Karuppiah, Thilakavathy Objective: To quantify circulating fetal DNA (fDNA) levels in the second and third trimesters of normal healthy pregnant individuals and pregnant women with the following clinical conditions: gestational diabetes mellitus (GDM), iron deficiency anemia and gestational hypertension (GHT). Methods: The SRY gene located on the Y chromosome was used as a unique fetal marker. The fDNA was extracted from maternal plasma and the SRY gene concentrations were measured by quantitative real-time polymerase chain reaction (PCR) amplification using TaqMan dual labeled probe system. Results: No significant differences were observed in the mean fDNA concentration between normal and GDM pregnancy samples (p > 0.05) and also between normal and anemic pregnancy samples (p > 0.05) in both trimesters, but significant differences were observed between the third trimester normal and GHT pregnancy samples (p = 0.001). GDM and iron deficiency anemia do not affect the levels of fDNA in maternal plasma while GHT significantly elevates the levels of fDNA in maternal plasma. Conclusions: Increased amount of circulating fDNA in maternal plasma could be used for early identification of adverse pregnancies. GDM and anemia do not affect the levels of fDNA in maternal plasma while GHT significantly elevates the levels of fDNA in maternal plasma. Hence, the elevated fDNA values could be used as a potential screening marker in pregnancies complicated with GHT but not with GDM and iron deficiency anemia. Taylor & Francis 2013 Article PeerReviewed Zamanpoor, Mansour and Rosli, Rozita and Yazid, Mohd Nazri and Husain, Zaheed and Nordin, Norshariza and Karuppiah, Thilakavathy (2013) Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies. The Journal of Maternal-Fetal & Neonatal Medicine, 26 (10). pp. 960-966. ISSN 1476-7058; ESSN: 1476-4954 http://www.tandfonline.com/doi/abs/10.3109/14767058.2013.766710 10.3109/14767058.2013.766710 |
| spellingShingle | Zamanpoor, Mansour Rosli, Rozita Yazid, Mohd Nazri Husain, Zaheed Nordin, Norshariza Karuppiah, Thilakavathy Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies |
| title | Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies |
| title_full | Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies |
| title_fullStr | Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies |
| title_full_unstemmed | Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies |
| title_short | Quantitative analysis of fetal DNA in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies |
| title_sort | quantitative analysis of fetal dna in maternal plasma in gestational diabetes mellitus, iron deficiency anemia and gestational hypertension pregnancies |
| url | http://psasir.upm.edu.my/id/eprint/29547/ http://psasir.upm.edu.my/id/eprint/29547/ http://psasir.upm.edu.my/id/eprint/29547/ |