Modified silver staining in 2DE improves protein detection even at extremely low sample concentration
The typical concentration of protein loaded varies from 0.13 to 1.40 μg/μL for a classical silver staining method in 2DE gel. Here, we present a simple modified classical silver staining method by modifying the silver impregnation and development reaction steps. This modified method detects the prot...
| Main Authors: | , , , , |
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| Format: | Article |
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Wiley-VCH Verlag
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29541/ |
| _version_ | 1848846427463090176 |
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| author | Liew, Yun Khoon Neela, Vasantha Kumari Awang Hamat, Rukman Amin Nordin, Syafinaz Chong, Pei Pei |
| author_facet | Liew, Yun Khoon Neela, Vasantha Kumari Awang Hamat, Rukman Amin Nordin, Syafinaz Chong, Pei Pei |
| author_sort | Liew, Yun Khoon |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The typical concentration of protein loaded varies from 0.13 to 1.40 μg/μL for a classical silver staining method in 2DE gel. Here, we present a simple modified classical silver staining method by modifying the silver impregnation and development reaction steps. This modified method detects the protein spots at extremely low loaded concentrations, ranging from 0.0048 to 0.0480 μg/μL. We recommend this modified silver staining as an excellent method for the limited biological samples used for silver-stained 2DE analysis. Altogether, the protocol takes close to two days from first dimension separation to second dimension separation, followed by silver staining, scanning, and analysis. |
| first_indexed | 2025-11-15T09:02:32Z |
| format | Article |
| id | upm-29541 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:02:32Z |
| publishDate | 2013 |
| publisher | Wiley-VCH Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-295412015-12-08T04:06:13Z http://psasir.upm.edu.my/id/eprint/29541/ Modified silver staining in 2DE improves protein detection even at extremely low sample concentration Liew, Yun Khoon Neela, Vasantha Kumari Awang Hamat, Rukman Amin Nordin, Syafinaz Chong, Pei Pei The typical concentration of protein loaded varies from 0.13 to 1.40 μg/μL for a classical silver staining method in 2DE gel. Here, we present a simple modified classical silver staining method by modifying the silver impregnation and development reaction steps. This modified method detects the protein spots at extremely low loaded concentrations, ranging from 0.0048 to 0.0480 μg/μL. We recommend this modified silver staining as an excellent method for the limited biological samples used for silver-stained 2DE analysis. Altogether, the protocol takes close to two days from first dimension separation to second dimension separation, followed by silver staining, scanning, and analysis. Wiley-VCH Verlag 2013-02 Article PeerReviewed Liew, Yun Khoon and Neela, Vasantha Kumari and Awang Hamat, Rukman and Amin Nordin, Syafinaz and Chong, Pei Pei (2013) Modified silver staining in 2DE improves protein detection even at extremely low sample concentration. Electrophoresis, 34 (3). pp. 397-400. ISSN 0173-0835; ESSN: 1522-2683 http://onlinelibrary.wiley.com/doi/10.1002/elps.201200380/abstract 10.1002/elps.201200380 |
| spellingShingle | Liew, Yun Khoon Neela, Vasantha Kumari Awang Hamat, Rukman Amin Nordin, Syafinaz Chong, Pei Pei Modified silver staining in 2DE improves protein detection even at extremely low sample concentration |
| title | Modified silver staining in 2DE improves protein detection even at extremely low sample concentration |
| title_full | Modified silver staining in 2DE improves protein detection even at extremely low sample concentration |
| title_fullStr | Modified silver staining in 2DE improves protein detection even at extremely low sample concentration |
| title_full_unstemmed | Modified silver staining in 2DE improves protein detection even at extremely low sample concentration |
| title_short | Modified silver staining in 2DE improves protein detection even at extremely low sample concentration |
| title_sort | modified silver staining in 2de improves protein detection even at extremely low sample concentration |
| url | http://psasir.upm.edu.my/id/eprint/29541/ http://psasir.upm.edu.my/id/eprint/29541/ http://psasir.upm.edu.my/id/eprint/29541/ |