Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patient in Saudi Arabia

One hundred and twenty methicillin-resistant Staphylococcus aureus (MRSA) isolated from cancer and non-cancer patients in Saudi Arabia were investigated for antibiotic resistance, virulence determinants and genotypes. The majority of MRSA isolates from cancer (n = 44, 73.3 %) and non-cancer patients...

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Main Authors: Alreshidi, Mateq Ali, Al-Salamah, Ali A., Awang Hamat, Rukman, Neela, Vasantha Kumari, Alsharari, Ahmad Subeh, Atshan, Salman Sahab, Alajlan, Hisham H., Shamsudin, Mariana Nor
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Published: Springer Verlag 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29539/
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author Alreshidi, Mateq Ali
Al-Salamah, Ali A.
Awang Hamat, Rukman
Neela, Vasantha Kumari
Alsharari, Ahmad Subeh
Atshan, Salman Sahab
Alajlan, Hisham H.
Shamsudin, Mariana Nor
author_facet Alreshidi, Mateq Ali
Al-Salamah, Ali A.
Awang Hamat, Rukman
Neela, Vasantha Kumari
Alsharari, Ahmad Subeh
Atshan, Salman Sahab
Alajlan, Hisham H.
Shamsudin, Mariana Nor
author_sort Alreshidi, Mateq Ali
building UPM Institutional Repository
collection Online Access
description One hundred and twenty methicillin-resistant Staphylococcus aureus (MRSA) isolated from cancer and non-cancer patients in Saudi Arabia were investigated for antibiotic resistance, virulence determinants and genotypes. The majority of MRSA isolates from cancer (n = 44, 73.3 %) and non-cancer patients (n = 34, 56.7 %) were multi-resistant to more than four classes of antibiotics. Virulence gene profiling showed that all strains were commonly positive for adhesin genes, except ebps and bbp genes, which were not detected in any isolate. Although the presence of adhesin genes varied slightly among MRSA isolates from cancer and non-cancer patients, these variations were not found to be statistically significant. In contrast, the presence of the toxin genes seb, sec, seg and sei was significantly elevated in MRSA strains isolated from cancer patients. Multilocus sequence typing (MLST) detected six and nine sequence types (STs) among isolates from cancer and non-cancer patients, respectively. Using spa typing, 12 and 25 types were detected, including four new types. The ability of different MRSA clones to become multi-resistant and their ability to acquire different virulence factors may contribute to their success as pathogens in individual groups of patients.
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spelling upm-295392015-12-08T12:25:02Z http://psasir.upm.edu.my/id/eprint/29539/ Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patient in Saudi Arabia Alreshidi, Mateq Ali Al-Salamah, Ali A. Awang Hamat, Rukman Neela, Vasantha Kumari Alsharari, Ahmad Subeh Atshan, Salman Sahab Alajlan, Hisham H. Shamsudin, Mariana Nor One hundred and twenty methicillin-resistant Staphylococcus aureus (MRSA) isolated from cancer and non-cancer patients in Saudi Arabia were investigated for antibiotic resistance, virulence determinants and genotypes. The majority of MRSA isolates from cancer (n = 44, 73.3 %) and non-cancer patients (n = 34, 56.7 %) were multi-resistant to more than four classes of antibiotics. Virulence gene profiling showed that all strains were commonly positive for adhesin genes, except ebps and bbp genes, which were not detected in any isolate. Although the presence of adhesin genes varied slightly among MRSA isolates from cancer and non-cancer patients, these variations were not found to be statistically significant. In contrast, the presence of the toxin genes seb, sec, seg and sei was significantly elevated in MRSA strains isolated from cancer patients. Multilocus sequence typing (MLST) detected six and nine sequence types (STs) among isolates from cancer and non-cancer patients, respectively. Using spa typing, 12 and 25 types were detected, including four new types. The ability of different MRSA clones to become multi-resistant and their ability to acquire different virulence factors may contribute to their success as pathogens in individual groups of patients. Springer Verlag 2013-06 Article PeerReviewed Alreshidi, Mateq Ali and Al-Salamah, Ali A. and Awang Hamat, Rukman and Neela, Vasantha Kumari and Alsharari, Ahmad Subeh and Atshan, Salman Sahab and Alajlan, Hisham H. and Shamsudin, Mariana Nor (2013) Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patient in Saudi Arabia. European Journal of Clinical Microbiology & Infectious Diseases, 32 (6). 755-761. ISSN 0934-9723; ESSN: 1435-4373 http://link.springer.com/article/10.1007%2Fs10096-012-1801-9 10.1007/s10096-012-1801-9
spellingShingle Alreshidi, Mateq Ali
Al-Salamah, Ali A.
Awang Hamat, Rukman
Neela, Vasantha Kumari
Alsharari, Ahmad Subeh
Atshan, Salman Sahab
Alajlan, Hisham H.
Shamsudin, Mariana Nor
Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patient in Saudi Arabia
title Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patient in Saudi Arabia
title_full Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patient in Saudi Arabia
title_fullStr Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patient in Saudi Arabia
title_full_unstemmed Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patient in Saudi Arabia
title_short Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patient in Saudi Arabia
title_sort genetic variation among methicillin-resistant staphylococcus aureus isolates from cancer patient in saudi arabia
url http://psasir.upm.edu.my/id/eprint/29539/
http://psasir.upm.edu.my/id/eprint/29539/
http://psasir.upm.edu.my/id/eprint/29539/