Nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice

The objective of this study was to investigate the nutritional compositions and antioxidant activities (AA) of non-polar and polar extracts of germinated brown rice (GBR). Nutritional compositions such as moisture, ash, carbohydrate, fat, protein and fibre were determined. Energy and minerals conten...

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Main Authors: Lim, See Meng, Goh, Yong Meng, Loh, Su Peng
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2016
Online Access:http://psasir.upm.edu.my/id/eprint/29537/
http://psasir.upm.edu.my/id/eprint/29537/1/07%20JTAS-0752-2015.pdf
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author Lim, See Meng
Goh, Yong Meng
Loh, Su Peng
author_facet Lim, See Meng
Goh, Yong Meng
Loh, Su Peng
author_sort Lim, See Meng
building UPM Institutional Repository
collection Online Access
description The objective of this study was to investigate the nutritional compositions and antioxidant activities (AA) of non-polar and polar extracts of germinated brown rice (GBR). Nutritional compositions such as moisture, ash, carbohydrate, fat, protein and fibre were determined. Energy and minerals content were determined by using bomb calorimetry and Atomic Absorption Spectroscopy (AAS), respectively. Total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were determined by Folin-Ciocalteu method and aluminium chloride colorimetric method. The AA was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and [2,2′-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] diammonium salt (ABTS) radical scavenging capacity assay, ferric reducing antioxidant potential (FRAP) assay, and β-carotene bleaching assay. Total energy content of GBR sample was 390.95±11.31 kcal/100g and carbohydrate (54.30±1.04 g/100 g) was the most abundant nutrient. The predominant minerals in the GBR sample were sodium, potassium and magnesium. The polar extract showed significantly higher (p<0.05) level in TPC, TFC and AA than non-polar extract except in β-carotene bleaching assay. Positive and strong correlations (r>0.90, p<0.001) existed between antioxidants (TPC and TFC) and AA. Therefore, polar extract was better than non-polar extract. The nutritional composition of GBR also provided an update for food composition database.
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spelling upm-295372016-08-02T02:58:11Z http://psasir.upm.edu.my/id/eprint/29537/ Nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice Lim, See Meng Goh, Yong Meng Loh, Su Peng The objective of this study was to investigate the nutritional compositions and antioxidant activities (AA) of non-polar and polar extracts of germinated brown rice (GBR). Nutritional compositions such as moisture, ash, carbohydrate, fat, protein and fibre were determined. Energy and minerals content were determined by using bomb calorimetry and Atomic Absorption Spectroscopy (AAS), respectively. Total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were determined by Folin-Ciocalteu method and aluminium chloride colorimetric method. The AA was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and [2,2′-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] diammonium salt (ABTS) radical scavenging capacity assay, ferric reducing antioxidant potential (FRAP) assay, and β-carotene bleaching assay. Total energy content of GBR sample was 390.95±11.31 kcal/100g and carbohydrate (54.30±1.04 g/100 g) was the most abundant nutrient. The predominant minerals in the GBR sample were sodium, potassium and magnesium. The polar extract showed significantly higher (p<0.05) level in TPC, TFC and AA than non-polar extract except in β-carotene bleaching assay. Positive and strong correlations (r>0.90, p<0.001) existed between antioxidants (TPC and TFC) and AA. Therefore, polar extract was better than non-polar extract. The nutritional composition of GBR also provided an update for food composition database. Universiti Putra Malaysia Press 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29537/1/07%20JTAS-0752-2015.pdf Lim, See Meng and Goh, Yong Meng and Loh, Su Peng (2016) Nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice. Pertanika Journal of Tropical Agricultural Science, 39 (2). pp. 219-233. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2039%20(2)%20May.%202016/07%20JTAS-0752-2015.pdf
spellingShingle Lim, See Meng
Goh, Yong Meng
Loh, Su Peng
Nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice
title Nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice
title_full Nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice
title_fullStr Nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice
title_full_unstemmed Nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice
title_short Nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice
title_sort nutritional compositions and antioxidant activities of non-polar and polar extracts of germinated brown rice
url http://psasir.upm.edu.my/id/eprint/29537/
http://psasir.upm.edu.my/id/eprint/29537/
http://psasir.upm.edu.my/id/eprint/29537/1/07%20JTAS-0752-2015.pdf