Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran

In the present study, effect of different supercritical fluid extractor (SFE) operational conditions i.e. pressure (300, 450, 600 bars), flow rate (10.0, 17.5, 25.0 g/min), and intervals of extraction (30, 90, 150 min) at 60 °C on protein content of defatted rice bran (DRB) samples, has been evaluat...

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Main Authors: Foong, Lian Chee, Iqbal, Shahid, Ismail, Maznah
Format: Article
Language:English
Published: The Chemical Society of Pakistan 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29524/
http://psasir.upm.edu.my/id/eprint/29524/1/Effects%20of%20supercritical%20fluid%20extraction%20conditions%20on%20yield%20of%20protein%20from%20defatted%20rice%20bran.pdf
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author Foong, Lian Chee
Iqbal, Shahid
Ismail, Maznah
author_facet Foong, Lian Chee
Iqbal, Shahid
Ismail, Maznah
author_sort Foong, Lian Chee
building UPM Institutional Repository
collection Online Access
description In the present study, effect of different supercritical fluid extractor (SFE) operational conditions i.e. pressure (300, 450, 600 bars), flow rate (10.0, 17.5, 25.0 g/min), and intervals of extraction (30, 90, 150 min) at 60 °C on protein content of defatted rice bran (DRB) samples, has been evaluated. The DRB samples were collected from SFE vessel and analyzed for their moisture, residual fat and protein contents. The protein content was determined by Kjeldahl method and ranged over 15.59 to 17.44%, while moisture content was determined by drying method and found to be ranging from 7.84 to 9.88%, and residual fat content was determined by Soxhlet method and varied over 4.67 to 18.94%. Differences were selectively significant (p > 0.05) among the protein, residual fat, and moisture contents, as function of extraction conditions. Highest yield of protein was obtained at 450 bars, 90 min extraction time, and 17.5 g/min flow rate, while the lowest at 300 bars, 30 min of extraction time, and 10.0 g/min flow rate. Negative correlation with p-value ≤ 0.05 was found between moisture content and residual fat content of DRB. However, no correlation could be observed among protein, moisture and residual fat contents of DRB, respectively. The study demonstrates that SFE conditions have non-significant impact on the yield of protein in defatted materials.
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spelling upm-295242016-07-12T09:23:41Z http://psasir.upm.edu.my/id/eprint/29524/ Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran Foong, Lian Chee Iqbal, Shahid Ismail, Maznah In the present study, effect of different supercritical fluid extractor (SFE) operational conditions i.e. pressure (300, 450, 600 bars), flow rate (10.0, 17.5, 25.0 g/min), and intervals of extraction (30, 90, 150 min) at 60 °C on protein content of defatted rice bran (DRB) samples, has been evaluated. The DRB samples were collected from SFE vessel and analyzed for their moisture, residual fat and protein contents. The protein content was determined by Kjeldahl method and ranged over 15.59 to 17.44%, while moisture content was determined by drying method and found to be ranging from 7.84 to 9.88%, and residual fat content was determined by Soxhlet method and varied over 4.67 to 18.94%. Differences were selectively significant (p > 0.05) among the protein, residual fat, and moisture contents, as function of extraction conditions. Highest yield of protein was obtained at 450 bars, 90 min extraction time, and 17.5 g/min flow rate, while the lowest at 300 bars, 30 min of extraction time, and 10.0 g/min flow rate. Negative correlation with p-value ≤ 0.05 was found between moisture content and residual fat content of DRB. However, no correlation could be observed among protein, moisture and residual fat contents of DRB, respectively. The study demonstrates that SFE conditions have non-significant impact on the yield of protein in defatted materials. The Chemical Society of Pakistan 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29524/1/Effects%20of%20supercritical%20fluid%20extraction%20conditions%20on%20yield%20of%20protein%20from%20defatted%20rice%20bran.pdf Foong, Lian Chee and Iqbal, Shahid and Ismail, Maznah (2013) Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran. Journal of the Chemical Society of Pakistan, 35 (1). pp. 192-197. ISSN 0253-5106 http://jcsp.org.pk/ViewByVolume.aspx?v=190&i=VOLUME%2035,%20NO1,%20FEB%202013
spellingShingle Foong, Lian Chee
Iqbal, Shahid
Ismail, Maznah
Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran
title Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran
title_full Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran
title_fullStr Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran
title_full_unstemmed Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran
title_short Effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran
title_sort effects of supercritical fluid extraction conditions on yield of protein from defatted rice bran
url http://psasir.upm.edu.my/id/eprint/29524/
http://psasir.upm.edu.my/id/eprint/29524/
http://psasir.upm.edu.my/id/eprint/29524/1/Effects%20of%20supercritical%20fluid%20extraction%20conditions%20on%20yield%20of%20protein%20from%20defatted%20rice%20bran.pdf