Genetic structure and preliminary findings of cryptic diversity of the Malaysian mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses
This study examines the population genetic structure of Tor tambroides, an important freshwater fish species in Malaysia, using fifteen polymorphic microsatellite loci and sequencing of 464 base pairs of the mitochondrial cytochrome c oxidase I (COI) gene. A total of 152 mahseer samples were collect...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29517/ http://psasir.upm.edu.my/id/eprint/29517/1/Genetic%20structure%20and%20preliminary%20findings%20of%20cryptic%20diversity%20of%20the%20Malaysian%20mahseer.pdf |
| _version_ | 1848846420427145216 |
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| author | Esa, Yuzine Abdul Rahim, Khairul Adha |
| author_facet | Esa, Yuzine Abdul Rahim, Khairul Adha |
| author_sort | Esa, Yuzine |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study examines the population genetic structure of Tor tambroides, an important freshwater fish species in Malaysia, using fifteen polymorphic microsatellite loci and sequencing of 464 base pairs of the mitochondrial cytochrome c oxidase I (COI) gene. A total of 152 mahseer samples were collected from eight populations throughout the Malaysia river system. Microsatellites results found high levels of intrapopulation variations, but mitochondrial COI results found high levels of interpopulations differentiation. The possible reasons for their discrepancies might be the varying influence of genetic drift on each marker or the small sample sizes used in most of the populations. The Kelantan population showed very low levels of genetic variations using both mitochondrial and microsatellite analyses. Phylogenetic analysis of the COI gene found a unique haplotype (ER8*), possibly representing a cryptic lineage of T. douronensis, from the Endau-Rompin population. Nevertheless, the inclusion of nuclear microsatellite analyses could not fully resolve the genetic identity of haplotype ER8* in the present study. Overall, the findings showed a serious need for more comprehensive and larger scale samplings, especially in remote river systems, in combination with molecular analyses using multiple markers, in order to discover more cryptic lineages or undescribed “genetic species” of mahseer. |
| first_indexed | 2025-11-15T09:02:26Z |
| format | Article |
| id | upm-29517 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:26Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-295172016-02-12T03:40:58Z http://psasir.upm.edu.my/id/eprint/29517/ Genetic structure and preliminary findings of cryptic diversity of the Malaysian mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses Esa, Yuzine Abdul Rahim, Khairul Adha This study examines the population genetic structure of Tor tambroides, an important freshwater fish species in Malaysia, using fifteen polymorphic microsatellite loci and sequencing of 464 base pairs of the mitochondrial cytochrome c oxidase I (COI) gene. A total of 152 mahseer samples were collected from eight populations throughout the Malaysia river system. Microsatellites results found high levels of intrapopulation variations, but mitochondrial COI results found high levels of interpopulations differentiation. The possible reasons for their discrepancies might be the varying influence of genetic drift on each marker or the small sample sizes used in most of the populations. The Kelantan population showed very low levels of genetic variations using both mitochondrial and microsatellite analyses. Phylogenetic analysis of the COI gene found a unique haplotype (ER8*), possibly representing a cryptic lineage of T. douronensis, from the Endau-Rompin population. Nevertheless, the inclusion of nuclear microsatellite analyses could not fully resolve the genetic identity of haplotype ER8* in the present study. Overall, the findings showed a serious need for more comprehensive and larger scale samplings, especially in remote river systems, in combination with molecular analyses using multiple markers, in order to discover more cryptic lineages or undescribed “genetic species” of mahseer. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29517/1/Genetic%20structure%20and%20preliminary%20findings%20of%20cryptic%20diversity%20of%20the%20Malaysian%20mahseer.pdf Esa, Yuzine and Abdul Rahim, Khairul Adha (2013) Genetic structure and preliminary findings of cryptic diversity of the Malaysian mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses. BioMed Research International, 2013. art. no. 170980. pp. 1-14. ISSN 1110-7243 http://dx.doi.org/10.1155/2013/170980 10.1155/2013/170980 |
| spellingShingle | Esa, Yuzine Abdul Rahim, Khairul Adha Genetic structure and preliminary findings of cryptic diversity of the Malaysian mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses |
| title | Genetic structure and preliminary findings of cryptic diversity of the Malaysian mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses |
| title_full | Genetic structure and preliminary findings of cryptic diversity of the Malaysian mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses |
| title_fullStr | Genetic structure and preliminary findings of cryptic diversity of the Malaysian mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses |
| title_full_unstemmed | Genetic structure and preliminary findings of cryptic diversity of the Malaysian mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses |
| title_short | Genetic structure and preliminary findings of cryptic diversity of the Malaysian mahseer (Tor tambroides Valenciennes: Cyprinidae) inferred from mitochondrial DNA and microsatellite analyses |
| title_sort | genetic structure and preliminary findings of cryptic diversity of the malaysian mahseer (tor tambroides valenciennes: cyprinidae) inferred from mitochondrial dna and microsatellite analyses |
| url | http://psasir.upm.edu.my/id/eprint/29517/ http://psasir.upm.edu.my/id/eprint/29517/ http://psasir.upm.edu.my/id/eprint/29517/ http://psasir.upm.edu.my/id/eprint/29517/1/Genetic%20structure%20and%20preliminary%20findings%20of%20cryptic%20diversity%20of%20the%20Malaysian%20mahseer.pdf |