Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia.
Motile Aeromonad septicemia is the main devastating disease in cultured fish and caused mainly by Aeromonas hydrophila. Development of pathogenic bacterial resistance caused by the excessive use of chemotherapeutics is the main disadvantage of antibiotic application. Thus, the sensitivity of certain...
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| Format: | Article |
| Language: | English English |
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Academic Journals
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29511/ http://psasir.upm.edu.my/id/eprint/29511/1/Herbal%20and%20antibiotic%20resistance%20of%20Aeromonas%20bacteria%20isolated%20from%20cultured%20fish%20in%20Egypt%20and%20Malaysia.pdf |
| _version_ | 1848846418748375040 |
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| author | M. S., Khairul Afizi B. S., Siti Fatimah Shamsudin, Mariana Nor Abdelhadi, Yasser Mohamed |
| author_facet | M. S., Khairul Afizi B. S., Siti Fatimah Shamsudin, Mariana Nor Abdelhadi, Yasser Mohamed |
| author_sort | M. S., Khairul Afizi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Motile Aeromonad septicemia is the main devastating disease in cultured fish and caused mainly by Aeromonas hydrophila. Development of pathogenic bacterial resistance caused by the excessive use of chemotherapeutics is the main disadvantage of antibiotic application. Thus, the sensitivity of certain commercial antibiotics and common herbs was evaluated against pathogenic A. hydrophila, A. sobria and A. caviae, isolated from Malaysian and Egyptian cultured fish, mainly tilapia. A suspension of freshly cultured isolates was prepared and spread over the Muller’s Hinton agar plates to study their sensitivity to both antibiotics and herbs. Double-fold dilution was used to determine the Minimal Inhibitory Concentration (MIC) for the effective herbs at 100, 50, 25, 12.5 and 6.25%. Results revealed high resistance of the 6 tested Aeromonas isolates against most of the screened antibiotics except Ciprofloxacin and Gentamicin. Regarding herbal sensitivity, only Origanum vulgare showed an efficacy and inhibition zone against all isolates except A. hydrophila isolated from cultured tilapia in Egypt which exhibited resistance to that herb. The MIC ranged from 25-55% for the Egyptian isolates (25% for A. caviae and 55% for A. sobria) and 24-30% for the Malaysian isolates (24% for A. hydrophila, 25% for A. caviae and 30% for A. sobria). This study recommends the mandate of effective vaccine development against A. hydrophila to protect cultured fish from that pathogen due to its high resistance for both herbs and antibiotics. |
| first_indexed | 2025-11-15T09:02:24Z |
| format | Article |
| id | upm-29511 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:02:24Z |
| publishDate | 2013 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-295112015-10-08T02:49:04Z http://psasir.upm.edu.my/id/eprint/29511/ Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia. M. S., Khairul Afizi B. S., Siti Fatimah Shamsudin, Mariana Nor Abdelhadi, Yasser Mohamed Motile Aeromonad septicemia is the main devastating disease in cultured fish and caused mainly by Aeromonas hydrophila. Development of pathogenic bacterial resistance caused by the excessive use of chemotherapeutics is the main disadvantage of antibiotic application. Thus, the sensitivity of certain commercial antibiotics and common herbs was evaluated against pathogenic A. hydrophila, A. sobria and A. caviae, isolated from Malaysian and Egyptian cultured fish, mainly tilapia. A suspension of freshly cultured isolates was prepared and spread over the Muller’s Hinton agar plates to study their sensitivity to both antibiotics and herbs. Double-fold dilution was used to determine the Minimal Inhibitory Concentration (MIC) for the effective herbs at 100, 50, 25, 12.5 and 6.25%. Results revealed high resistance of the 6 tested Aeromonas isolates against most of the screened antibiotics except Ciprofloxacin and Gentamicin. Regarding herbal sensitivity, only Origanum vulgare showed an efficacy and inhibition zone against all isolates except A. hydrophila isolated from cultured tilapia in Egypt which exhibited resistance to that herb. The MIC ranged from 25-55% for the Egyptian isolates (25% for A. caviae and 55% for A. sobria) and 24-30% for the Malaysian isolates (24% for A. hydrophila, 25% for A. caviae and 30% for A. sobria). This study recommends the mandate of effective vaccine development against A. hydrophila to protect cultured fish from that pathogen due to its high resistance for both herbs and antibiotics. Academic Journals 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29511/1/Herbal%20and%20antibiotic%20resistance%20of%20Aeromonas%20bacteria%20isolated%20from%20cultured%20fish%20in%20Egypt%20and%20Malaysia.pdf M. S., Khairul Afizi and B. S., Siti Fatimah and Shamsudin, Mariana Nor and Abdelhadi, Yasser Mohamed (2013) Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia. Journal of Fisheries and Aquatic Science, 8 (2). pp. 425-429. ISSN 1816-4927; ESSN: 1996-0751 http://scialert.net/archivedetails.php?issn=1816-4927&issueno=44 10.3923/jfas.2013.425.429 English |
| spellingShingle | M. S., Khairul Afizi B. S., Siti Fatimah Shamsudin, Mariana Nor Abdelhadi, Yasser Mohamed Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia. |
| title | Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia. |
| title_full | Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia. |
| title_fullStr | Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia. |
| title_full_unstemmed | Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia. |
| title_short | Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia. |
| title_sort | herbal and antibiotic resistance of aeromonas bacteria isolated from cultured fish in egypt and malaysia. |
| url | http://psasir.upm.edu.my/id/eprint/29511/ http://psasir.upm.edu.my/id/eprint/29511/ http://psasir.upm.edu.my/id/eprint/29511/ http://psasir.upm.edu.my/id/eprint/29511/1/Herbal%20and%20antibiotic%20resistance%20of%20Aeromonas%20bacteria%20isolated%20from%20cultured%20fish%20in%20Egypt%20and%20Malaysia.pdf |