Effects of GnRHa on plasma sex steroid hormones of river catfish Hemibagrus nemurus (Valenciennes 1840)
The effect of gonadotropin releasing hormone analogue (GnRHa) on plasma sex steroid hormones of river catfish Hemibagrus nemurus was studied. Enzyme linked immunosorbent assay (ELISA) was used to measure the hormones. The fish were treated with saline (control) and GnRHa at various doses of 5 µg/kg,...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29493/ http://psasir.upm.edu.my/id/eprint/29493/1/Effects%20of%20GnRHa%20on%20plasma%20sex%20steroid%20hormones%20of%20river%20catfish%20Hemibagrus%20nemurus.pdf |
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| author | Adebiyi, Fatimat Adenike Siraj, Siti Shapor Harmin, Sharr Azni Christianus, Annie |
| author_facet | Adebiyi, Fatimat Adenike Siraj, Siti Shapor Harmin, Sharr Azni Christianus, Annie |
| author_sort | Adebiyi, Fatimat Adenike |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The effect of gonadotropin releasing hormone analogue (GnRHa) on plasma sex steroid hormones of river catfish Hemibagrus nemurus was studied. Enzyme linked immunosorbent assay (ELISA) was used to measure the hormones. The fish were treated with saline (control) and GnRHa at various doses of 5 µg/kg, 20 µg/kg and 50 µg/kg body weight (BW) of fish. Blood samples were collected at 0, 6, 12 and 24 h post hormone administration. The results showed that GnRHa elevated the plasma sex steroid hormones even at a low dose of 5 µg/kg. Significant increase (p< 0.05) in plasma sex steroid levels were observed with 20 µg/kg and 50 µg/kg BW GnRHa treatments and a dose of 5 µg/kg BW GnRHa produced a slow response to steroidogenesis. Treatment with 50 µg/kg BW GnRHa produced the best result. The highest level of testosterone, 11-ketotestosterone and 17β-estradiol were observed at 24 h for all treatments. The results indicated that GnRHa increased steroid production in the plasma of H. nemurus. Therefore, it can be used as an inducing agent for the control of reproduction in H. nemurus. |
| first_indexed | 2025-11-15T09:02:19Z |
| format | Article |
| id | upm-29493 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:19Z |
| publishDate | 2013 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-294932018-10-16T03:17:08Z http://psasir.upm.edu.my/id/eprint/29493/ Effects of GnRHa on plasma sex steroid hormones of river catfish Hemibagrus nemurus (Valenciennes 1840) Adebiyi, Fatimat Adenike Siraj, Siti Shapor Harmin, Sharr Azni Christianus, Annie The effect of gonadotropin releasing hormone analogue (GnRHa) on plasma sex steroid hormones of river catfish Hemibagrus nemurus was studied. Enzyme linked immunosorbent assay (ELISA) was used to measure the hormones. The fish were treated with saline (control) and GnRHa at various doses of 5 µg/kg, 20 µg/kg and 50 µg/kg body weight (BW) of fish. Blood samples were collected at 0, 6, 12 and 24 h post hormone administration. The results showed that GnRHa elevated the plasma sex steroid hormones even at a low dose of 5 µg/kg. Significant increase (p< 0.05) in plasma sex steroid levels were observed with 20 µg/kg and 50 µg/kg BW GnRHa treatments and a dose of 5 µg/kg BW GnRHa produced a slow response to steroidogenesis. Treatment with 50 µg/kg BW GnRHa produced the best result. The highest level of testosterone, 11-ketotestosterone and 17β-estradiol were observed at 24 h for all treatments. The results indicated that GnRHa increased steroid production in the plasma of H. nemurus. Therefore, it can be used as an inducing agent for the control of reproduction in H. nemurus. Penerbit Universiti Kebangsaan Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29493/1/Effects%20of%20GnRHa%20on%20plasma%20sex%20steroid%20hormones%20of%20river%20catfish%20Hemibagrus%20nemurus.pdf Adebiyi, Fatimat Adenike and Siraj, Siti Shapor and Harmin, Sharr Azni and Christianus, Annie (2013) Effects of GnRHa on plasma sex steroid hormones of river catfish Hemibagrus nemurus (Valenciennes 1840). Sains Malaysiana, 42 (5). pp. 635-642. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol42num5_2013/contentsVol42num5_2013.html |
| spellingShingle | Adebiyi, Fatimat Adenike Siraj, Siti Shapor Harmin, Sharr Azni Christianus, Annie Effects of GnRHa on plasma sex steroid hormones of river catfish Hemibagrus nemurus (Valenciennes 1840) |
| title | Effects of GnRHa on plasma sex steroid hormones of river catfish Hemibagrus nemurus (Valenciennes 1840) |
| title_full | Effects of GnRHa on plasma sex steroid hormones of river catfish Hemibagrus nemurus (Valenciennes 1840) |
| title_fullStr | Effects of GnRHa on plasma sex steroid hormones of river catfish Hemibagrus nemurus (Valenciennes 1840) |
| title_full_unstemmed | Effects of GnRHa on plasma sex steroid hormones of river catfish Hemibagrus nemurus (Valenciennes 1840) |
| title_short | Effects of GnRHa on plasma sex steroid hormones of river catfish Hemibagrus nemurus (Valenciennes 1840) |
| title_sort | effects of gnrha on plasma sex steroid hormones of river catfish hemibagrus nemurus (valenciennes 1840) |
| url | http://psasir.upm.edu.my/id/eprint/29493/ http://psasir.upm.edu.my/id/eprint/29493/ http://psasir.upm.edu.my/id/eprint/29493/1/Effects%20of%20GnRHa%20on%20plasma%20sex%20steroid%20hormones%20of%20river%20catfish%20Hemibagrus%20nemurus.pdf |