Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians
Leptin is known as the adipose peptide hormone. It plays an important role in the regulation of body fat and inhibits food intake by its action. Moreover, it is believed that leptin level deductions might be the cause of obesity and may play an important role in the development of Type 2 Diabetes Me...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29466/ http://psasir.upm.edu.my/id/eprint/29466/1/29466.pdf |
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| author | Etemad, Ali Ramachandran, Vasudevan Pishva, Seyyed Reza Heidari, Farzad Abdul Aziz, Ahmad Fazli Mohamed Yusof, Ahmad Khairuddin Chong, Pei Pei Ismail, Patimah |
| author_facet | Etemad, Ali Ramachandran, Vasudevan Pishva, Seyyed Reza Heidari, Farzad Abdul Aziz, Ahmad Fazli Mohamed Yusof, Ahmad Khairuddin Chong, Pei Pei Ismail, Patimah |
| author_sort | Etemad, Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Leptin is known as the adipose peptide hormone. It plays an important role in the regulation of body fat and inhibits food intake by its action. Moreover, it is believed that leptin level deductions might be the cause of obesity and may play an important role in the development of Type 2 Diabetes Mellitus (T2DM), as well as in cardiovascular diseases (CVD). The Leptin Receptor (LEPR) gene and its polymorphisms have not been extensively studied in relation to the T2DM and its complications in various populations. In this study, we have determined the association of Gln223Agr loci of LEPR gene in three ethnic groups of Malaysia, namely: Malays, Chinese and Indians. A total of 284 T2DM subjects and 281 healthy individuals were recruited based on International Diabetes Federation (IDF) criteria. Genomic DNA was extracted from the buccal specimens of the subjects. The commercial polymerase chain reaction (PCR) method was carried out by proper restriction enzyme MSP I to both amplify and digest the Gln223Agr polymorphism. The p-value among the three studied races was 0.057, 0.011 and 0.095, respectively. The values such as age, WHR, FPG, HbA1C, LDL, HDL, Chol and Family History were significantly different among the subjects with Gln223Agr polymorphism of LEPR (p < 0.05). |
| first_indexed | 2025-11-15T09:02:12Z |
| format | Article |
| id | upm-29466 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:12Z |
| publishDate | 2013 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-294662016-07-12T09:39:14Z http://psasir.upm.edu.my/id/eprint/29466/ Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians Etemad, Ali Ramachandran, Vasudevan Pishva, Seyyed Reza Heidari, Farzad Abdul Aziz, Ahmad Fazli Mohamed Yusof, Ahmad Khairuddin Chong, Pei Pei Ismail, Patimah Leptin is known as the adipose peptide hormone. It plays an important role in the regulation of body fat and inhibits food intake by its action. Moreover, it is believed that leptin level deductions might be the cause of obesity and may play an important role in the development of Type 2 Diabetes Mellitus (T2DM), as well as in cardiovascular diseases (CVD). The Leptin Receptor (LEPR) gene and its polymorphisms have not been extensively studied in relation to the T2DM and its complications in various populations. In this study, we have determined the association of Gln223Agr loci of LEPR gene in three ethnic groups of Malaysia, namely: Malays, Chinese and Indians. A total of 284 T2DM subjects and 281 healthy individuals were recruited based on International Diabetes Federation (IDF) criteria. Genomic DNA was extracted from the buccal specimens of the subjects. The commercial polymerase chain reaction (PCR) method was carried out by proper restriction enzyme MSP I to both amplify and digest the Gln223Agr polymorphism. The p-value among the three studied races was 0.057, 0.011 and 0.095, respectively. The values such as age, WHR, FPG, HbA1C, LDL, HDL, Chol and Family History were significantly different among the subjects with Gln223Agr polymorphism of LEPR (p < 0.05). MDPI 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29466/1/29466.pdf Etemad, Ali and Ramachandran, Vasudevan and Pishva, Seyyed Reza and Heidari, Farzad and Abdul Aziz, Ahmad Fazli and Mohamed Yusof, Ahmad Khairuddin and Chong, Pei Pei and Ismail, Patimah (2013) Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians. International Journal of Molecular Sciences, 14 (9). pp. 19230-19244. ISSN 1422-0067 http://www.mdpi.com/1422-0067/14/9/19230 10.3390/ijms140919230 |
| spellingShingle | Etemad, Ali Ramachandran, Vasudevan Pishva, Seyyed Reza Heidari, Farzad Abdul Aziz, Ahmad Fazli Mohamed Yusof, Ahmad Khairuddin Chong, Pei Pei Ismail, Patimah Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians |
| title | Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians |
| title_full | Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians |
| title_fullStr | Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians |
| title_full_unstemmed | Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians |
| title_short | Analysis of Gln223Agr polymorphism of leptin receptor gene in type II diabetic mellitus subjects among Malaysians |
| title_sort | analysis of gln223agr polymorphism of leptin receptor gene in type ii diabetic mellitus subjects among malaysians |
| url | http://psasir.upm.edu.my/id/eprint/29466/ http://psasir.upm.edu.my/id/eprint/29466/ http://psasir.upm.edu.my/id/eprint/29466/ http://psasir.upm.edu.my/id/eprint/29466/1/29466.pdf |