In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems
This study focuses on the establishment of in vitro tuberization of Chlorophytum borivilianum using solid and liquid culture systems. A high in vitro tuberization rate on solid and stationary liquid Murashige and Skoog media was observed in the presence of 60 g l−1 sucrose with 950, 1,265 and 1,580...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29457/ http://psasir.upm.edu.my/id/eprint/29457/1/29457.pdf |
| _version_ | 1848846404178411520 |
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| author | Ashraf, Mehdi Farshad Abdul Aziz, Maheran Abdul Kadir, Mihdzar Stanslas, Johnson Farokhian, Elmira |
| author_facet | Ashraf, Mehdi Farshad Abdul Aziz, Maheran Abdul Kadir, Mihdzar Stanslas, Johnson Farokhian, Elmira |
| author_sort | Ashraf, Mehdi Farshad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study focuses on the establishment of in vitro tuberization of Chlorophytum borivilianum using solid and liquid culture systems. A high in vitro tuberization rate on solid and stationary liquid Murashige and Skoog media was observed in the presence of 60 g l−1 sucrose with 950, 1,265 and 1,580 µM 2-chloroethyl-trimethylammonium chloride (CCC). Application of a higher sucrose concentration of 90 g l−1 showed a negative interaction with CCC on in vitro tuber number and days to in vitro tuber induction. For economic feasibility, 950 µM CCC with 60 g l−1 sucrose was chosen as the best combination for in vitro tuberization in both solid and stationary liquid media. For optimization of in vitro tuber production, a comparison between solid, stationary liquid and shake liquid culture was carried out. Liquid culture with shaking at 80 r.p.m. resulted in a >2.5-fold increase in in vitro tuber production compared with solid culture. |
| first_indexed | 2025-11-15T09:02:10Z |
| format | Article |
| id | upm-29457 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:10Z |
| publishDate | 2013 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-294572016-07-12T09:35:17Z http://psasir.upm.edu.my/id/eprint/29457/ In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems Ashraf, Mehdi Farshad Abdul Aziz, Maheran Abdul Kadir, Mihdzar Stanslas, Johnson Farokhian, Elmira This study focuses on the establishment of in vitro tuberization of Chlorophytum borivilianum using solid and liquid culture systems. A high in vitro tuberization rate on solid and stationary liquid Murashige and Skoog media was observed in the presence of 60 g l−1 sucrose with 950, 1,265 and 1,580 µM 2-chloroethyl-trimethylammonium chloride (CCC). Application of a higher sucrose concentration of 90 g l−1 showed a negative interaction with CCC on in vitro tuber number and days to in vitro tuber induction. For economic feasibility, 950 µM CCC with 60 g l−1 sucrose was chosen as the best combination for in vitro tuberization in both solid and stationary liquid media. For optimization of in vitro tuber production, a comparison between solid, stationary liquid and shake liquid culture was carried out. Liquid culture with shaking at 80 r.p.m. resulted in a >2.5-fold increase in in vitro tuber production compared with solid culture. Oxford University Press 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29457/1/29457.pdf Ashraf, Mehdi Farshad and Abdul Aziz, Maheran and Abdul Kadir, Mihdzar and Stanslas, Johnson and Farokhian, Elmira (2013) In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems. Plant & Cell Physiology, 54 (8). pp. 1356-1364. ISSN 0032-0781; ESSN: 1471-9053 http://pcp.oxfordjournals.org/content/54/8/1356.abstract 10.1093/pcp/pct083 |
| spellingShingle | Ashraf, Mehdi Farshad Abdul Aziz, Maheran Abdul Kadir, Mihdzar Stanslas, Johnson Farokhian, Elmira In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems |
| title | In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems |
| title_full | In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems |
| title_fullStr | In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems |
| title_full_unstemmed | In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems |
| title_short | In vitro tuberization of Chlorophytum borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems |
| title_sort | in vitro tuberization of chlorophytum borivilianum sant & fern (safed musli) as influenced by sucrose, ccc and culture systems |
| url | http://psasir.upm.edu.my/id/eprint/29457/ http://psasir.upm.edu.my/id/eprint/29457/ http://psasir.upm.edu.my/id/eprint/29457/ http://psasir.upm.edu.my/id/eprint/29457/1/29457.pdf |