Leaf spot on lettuce (Lactuca sativa) caused by Stemphylium solani, a new disease in Malaysia.
In June 2011, lettuce (Lactuca sativa) plants cultivated in major lettuce growing areas in Malaysia, including the Pahang and Johor states, had extensive leaf spots. In severe cases, disease incidence was recorded to be more than 80%. Symptoms on 50 plants were initially water-soaked spots (1-2 mm i...
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| Format: | Article |
| Language: | English English |
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American Phytopathological Society
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29451/ http://psasir.upm.edu.my/id/eprint/29451/1/Leaf%20spot%20on%20lettuce.pdf |
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| author | Nasehi, Abbas Kadir, Jugah Esfahani, Mehdi Nasr Mahmodi, Farshid Ghadirian, Hajar Ashtiani, Farnaz Abed Soleimani, N. |
| author_facet | Nasehi, Abbas Kadir, Jugah Esfahani, Mehdi Nasr Mahmodi, Farshid Ghadirian, Hajar Ashtiani, Farnaz Abed Soleimani, N. |
| author_sort | Nasehi, Abbas |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In June 2011, lettuce (Lactuca sativa) plants cultivated in major lettuce growing areas in Malaysia, including the Pahang and Johor states, had extensive leaf spots. In severe cases, disease incidence was recorded to be more than 80%. Symptoms on 50 plants were initially water-soaked spots (1-2 mm in diameter) on leaves, which then became circular spots spreading over much of the leaves. Main lettuce growing areas infected by the pathogen in the aforementioned states were investigated and the pathogen was isolated onto potato dextrose agar. Fourteen isolates were identified as Stemphylium solani based on morphological criteria. To confirm morphological characterization, the DNA of the fungus was extracted from mycelium and PCR was conducted using universal primers ITS5 (5′-GGAAGTAAAAGTCGTAACAAGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′), which amplified the internal transcribed spacer (ITS) region of rDNA. The sequencing result was subjected to BLAST analysis, revealing a 99% similarity to other published sequences in the GenBank database (GenBank accession nos. AF203451 and HQ840713). This is thought to be the first report of Stemphylium solani on lettuce in Malaysia. |
| first_indexed | 2025-11-15T09:02:09Z |
| format | Article |
| id | upm-29451 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:02:09Z |
| publishDate | 2013 |
| publisher | American Phytopathological Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-294512015-12-08T03:00:55Z http://psasir.upm.edu.my/id/eprint/29451/ Leaf spot on lettuce (Lactuca sativa) caused by Stemphylium solani, a new disease in Malaysia. Nasehi, Abbas Kadir, Jugah Esfahani, Mehdi Nasr Mahmodi, Farshid Ghadirian, Hajar Ashtiani, Farnaz Abed Soleimani, N. In June 2011, lettuce (Lactuca sativa) plants cultivated in major lettuce growing areas in Malaysia, including the Pahang and Johor states, had extensive leaf spots. In severe cases, disease incidence was recorded to be more than 80%. Symptoms on 50 plants were initially water-soaked spots (1-2 mm in diameter) on leaves, which then became circular spots spreading over much of the leaves. Main lettuce growing areas infected by the pathogen in the aforementioned states were investigated and the pathogen was isolated onto potato dextrose agar. Fourteen isolates were identified as Stemphylium solani based on morphological criteria. To confirm morphological characterization, the DNA of the fungus was extracted from mycelium and PCR was conducted using universal primers ITS5 (5′-GGAAGTAAAAGTCGTAACAAGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′), which amplified the internal transcribed spacer (ITS) region of rDNA. The sequencing result was subjected to BLAST analysis, revealing a 99% similarity to other published sequences in the GenBank database (GenBank accession nos. AF203451 and HQ840713). This is thought to be the first report of Stemphylium solani on lettuce in Malaysia. American Phytopathological Society 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29451/1/Leaf%20spot%20on%20lettuce.pdf Nasehi, Abbas and Kadir, Jugah and Esfahani, Mehdi Nasr and Mahmodi, Farshid and Ghadirian, Hajar and Ashtiani, Farnaz Abed and Soleimani, N. (2013) Leaf spot on lettuce (Lactuca sativa) caused by Stemphylium solani, a new disease in Malaysia. Plant Disease, 97 (5). 689.3. ISSN 0191-2917 http://www.apsnet.org/publications/plantdisease/2013/May/Pages/97_5_689.3.aspx 10.1094/PDIS-10-12-0902-PDN English |
| spellingShingle | Nasehi, Abbas Kadir, Jugah Esfahani, Mehdi Nasr Mahmodi, Farshid Ghadirian, Hajar Ashtiani, Farnaz Abed Soleimani, N. Leaf spot on lettuce (Lactuca sativa) caused by Stemphylium solani, a new disease in Malaysia. |
| title | Leaf spot on lettuce (Lactuca sativa) caused by Stemphylium solani, a new disease in Malaysia. |
| title_full | Leaf spot on lettuce (Lactuca sativa) caused by Stemphylium solani, a new disease in Malaysia. |
| title_fullStr | Leaf spot on lettuce (Lactuca sativa) caused by Stemphylium solani, a new disease in Malaysia. |
| title_full_unstemmed | Leaf spot on lettuce (Lactuca sativa) caused by Stemphylium solani, a new disease in Malaysia. |
| title_short | Leaf spot on lettuce (Lactuca sativa) caused by Stemphylium solani, a new disease in Malaysia. |
| title_sort | leaf spot on lettuce (lactuca sativa) caused by stemphylium solani, a new disease in malaysia. |
| url | http://psasir.upm.edu.my/id/eprint/29451/ http://psasir.upm.edu.my/id/eprint/29451/ http://psasir.upm.edu.my/id/eprint/29451/ http://psasir.upm.edu.my/id/eprint/29451/1/Leaf%20spot%20on%20lettuce.pdf |