Effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of Exserohilum longirostratum.

The effects of carbon and nitrogen sources and the carbon-to-nitrogen ratio on the growth and sporulation of Exserohilum longirostratum were evaluated. Rice flour and malt extracts as the carbon and nitrogen sources, respectively, produced the greatest amount of mycelium. Sources that produced the m...

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Main Authors: Kadir, Jugah, Mohd Saud, Halimi, Ng, S. C., Abdul Rahim, Anuar, Mohd Din, Bibi Nazihah
Format: Article
Language:English
English
Published: Universiti Sultan Zainal Abidin 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29442/
http://psasir.upm.edu.my/id/eprint/29442/1/Effect%20of%20carbon%20and%20nitrogen%20sources%20and%20carbon.pdf
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author Kadir, Jugah
Mohd Saud, Halimi
Ng, S. C.
Abdul Rahim, Anuar
Mohd Din, Bibi Nazihah
author_facet Kadir, Jugah
Mohd Saud, Halimi
Ng, S. C.
Abdul Rahim, Anuar
Mohd Din, Bibi Nazihah
author_sort Kadir, Jugah
building UPM Institutional Repository
collection Online Access
description The effects of carbon and nitrogen sources and the carbon-to-nitrogen ratio on the growth and sporulation of Exserohilum longirostratum were evaluated. Rice flour and malt extracts as the carbon and nitrogen sources, respectively, produced the greatest amount of mycelium. Sources that produced the most biomass were chosen as carbon and nitrogen sources in a C:N ratio test. However, in further experiments, glucose was chosen as the carbon source for the C:N ratio test because rice flour was found to be easily contaminated. Under the C:N ratio test (fixed carbon test), the highest spore production was obtained with a 5:1 ratio (4.78 × 106 spores/mL) and the highest biomass production was obtained with a 7.5:1 ratio (4.66 g/100 mL). In the fixed nitrogen test, 7.5:1 ratio provided the greatest output (4.08 × 106 spores/mL) whereas a 5:1 ratio produced the most biomass (4.33 g/100 mL). Meanwhile, the control which consisted of V8 agar without additional carbon and nitrogen source produced 1.07 × 106 spores/mL. These results provide information on the influence of carbon and nitrogen source and the C:N ratio that can be used in media for optimum growth and spore yield.
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spelling upm-294422015-09-18T02:31:56Z http://psasir.upm.edu.my/id/eprint/29442/ Effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of Exserohilum longirostratum. Kadir, Jugah Mohd Saud, Halimi Ng, S. C. Abdul Rahim, Anuar Mohd Din, Bibi Nazihah The effects of carbon and nitrogen sources and the carbon-to-nitrogen ratio on the growth and sporulation of Exserohilum longirostratum were evaluated. Rice flour and malt extracts as the carbon and nitrogen sources, respectively, produced the greatest amount of mycelium. Sources that produced the most biomass were chosen as carbon and nitrogen sources in a C:N ratio test. However, in further experiments, glucose was chosen as the carbon source for the C:N ratio test because rice flour was found to be easily contaminated. Under the C:N ratio test (fixed carbon test), the highest spore production was obtained with a 5:1 ratio (4.78 × 106 spores/mL) and the highest biomass production was obtained with a 7.5:1 ratio (4.66 g/100 mL). In the fixed nitrogen test, 7.5:1 ratio provided the greatest output (4.08 × 106 spores/mL) whereas a 5:1 ratio produced the most biomass (4.33 g/100 mL). Meanwhile, the control which consisted of V8 agar without additional carbon and nitrogen source produced 1.07 × 106 spores/mL. These results provide information on the influence of carbon and nitrogen source and the C:N ratio that can be used in media for optimum growth and spore yield. Universiti Sultan Zainal Abidin 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29442/1/Effect%20of%20carbon%20and%20nitrogen%20sources%20and%20carbon.pdf Kadir, Jugah and Mohd Saud, Halimi and Ng, S. C. and Abdul Rahim, Anuar and Mohd Din, Bibi Nazihah (2013) Effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of Exserohilum longirostratum. Journal of Agrobiotechnology, 4. pp. 57-67. ISSN 1985-5133; ESSN: 2180-1983 http://www.journal.unisza.edu.my/index.php/agrobiotechnology/issue/view/4/showToc English
spellingShingle Kadir, Jugah
Mohd Saud, Halimi
Ng, S. C.
Abdul Rahim, Anuar
Mohd Din, Bibi Nazihah
Effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of Exserohilum longirostratum.
title Effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of Exserohilum longirostratum.
title_full Effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of Exserohilum longirostratum.
title_fullStr Effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of Exserohilum longirostratum.
title_full_unstemmed Effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of Exserohilum longirostratum.
title_short Effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of Exserohilum longirostratum.
title_sort effect of carbon and nitrogen sources and carbon-to-nitrogen ratio on production of exserohilum longirostratum.
url http://psasir.upm.edu.my/id/eprint/29442/
http://psasir.upm.edu.my/id/eprint/29442/
http://psasir.upm.edu.my/id/eprint/29442/1/Effect%20of%20carbon%20and%20nitrogen%20sources%20and%20carbon.pdf