Comparison of effect of sex hormone manipulation during neonatal period, on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 in hippocampus and frontal cortex of male and female rats.

It has long been known that spatial memory and the ability to navigate through space are sexually dimorphic traits among mammals, and numerous studies have shown that these traits can be altered by means of sex hormone manipulation. Hippocampus, the main organ involved in this kind of memory, has sp...

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Main Authors: Karimi, B., Mohd Noor, Hafidzi, Panandam, Jothi Malar, Nor Hussein, Fuzina
Format: Article
Language:English
English
Published: Biolife 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29435/
http://psasir.upm.edu.my/id/eprint/29435/1/Comparison%20of%20effect%20of%20sex%20hormone%20manipulation%20during%20neonatal%20period.pdf
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author Karimi, B.
Mohd Noor, Hafidzi
Panandam, Jothi Malar
Nor Hussein, Fuzina
author_facet Karimi, B.
Mohd Noor, Hafidzi
Panandam, Jothi Malar
Nor Hussein, Fuzina
author_sort Karimi, B.
building UPM Institutional Repository
collection Online Access
description It has long been known that spatial memory and the ability to navigate through space are sexually dimorphic traits among mammals, and numerous studies have shown that these traits can be altered by means of sex hormone manipulation. Hippocampus, the main organ involved in this kind of memory, has specific signature genes with high expression level compared to other regions of the brain. Based on their expression levels and the role that products of these genes can play in processes like signal transduction, mediation of hormone effects and long term potentiation, these genes can be considered as genes necessary for routine tasks of hippocampus. Male and female rat pups were injected with estradiol and testosterone respectively. at early stage of their lives to examine the effect of sex hormone manipulation on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 using comparative quantitative real-time polymerase chain reaction. The results showed that expressions of these genes are strongly influenced by sex hormones in both the frontal cortex and hippocampus, especially in male hippocampus, in which expression of all genes were up-regulated. Htr5b was the only gene that was affected only in the males. Expression of Mas1 was contrary to expectations, showed stronger changes in its expression in cortex than in hippocampus. Nr3c2 was down regulated in all samples but up regulated in male hippocampus, and Slc9a4 also showed a huge up-regulation in male hippocampus compared to other samples.
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language English
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spelling upm-294352015-09-18T02:26:06Z http://psasir.upm.edu.my/id/eprint/29435/ Comparison of effect of sex hormone manipulation during neonatal period, on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 in hippocampus and frontal cortex of male and female rats. Karimi, B. Mohd Noor, Hafidzi Panandam, Jothi Malar Nor Hussein, Fuzina It has long been known that spatial memory and the ability to navigate through space are sexually dimorphic traits among mammals, and numerous studies have shown that these traits can be altered by means of sex hormone manipulation. Hippocampus, the main organ involved in this kind of memory, has specific signature genes with high expression level compared to other regions of the brain. Based on their expression levels and the role that products of these genes can play in processes like signal transduction, mediation of hormone effects and long term potentiation, these genes can be considered as genes necessary for routine tasks of hippocampus. Male and female rat pups were injected with estradiol and testosterone respectively. at early stage of their lives to examine the effect of sex hormone manipulation on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 using comparative quantitative real-time polymerase chain reaction. The results showed that expressions of these genes are strongly influenced by sex hormones in both the frontal cortex and hippocampus, especially in male hippocampus, in which expression of all genes were up-regulated. Htr5b was the only gene that was affected only in the males. Expression of Mas1 was contrary to expectations, showed stronger changes in its expression in cortex than in hippocampus. Nr3c2 was down regulated in all samples but up regulated in male hippocampus, and Slc9a4 also showed a huge up-regulation in male hippocampus compared to other samples. Biolife 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29435/1/Comparison%20of%20effect%20of%20sex%20hormone%20manipulation%20during%20neonatal%20period.pdf Karimi, B. and Mohd Noor, Hafidzi and Panandam, Jothi Malar and Nor Hussein, Fuzina (2013) Comparison of effect of sex hormone manipulation during neonatal period, on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 in hippocampus and frontal cortex of male and female rats. Journal of Biological Regulators & Homeostatic Agents, 27 (3). pp. 869-874. ISSN 0393-974 English
spellingShingle Karimi, B.
Mohd Noor, Hafidzi
Panandam, Jothi Malar
Nor Hussein, Fuzina
Comparison of effect of sex hormone manipulation during neonatal period, on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 in hippocampus and frontal cortex of male and female rats.
title Comparison of effect of sex hormone manipulation during neonatal period, on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 in hippocampus and frontal cortex of male and female rats.
title_full Comparison of effect of sex hormone manipulation during neonatal period, on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 in hippocampus and frontal cortex of male and female rats.
title_fullStr Comparison of effect of sex hormone manipulation during neonatal period, on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 in hippocampus and frontal cortex of male and female rats.
title_full_unstemmed Comparison of effect of sex hormone manipulation during neonatal period, on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 in hippocampus and frontal cortex of male and female rats.
title_short Comparison of effect of sex hormone manipulation during neonatal period, on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1 in hippocampus and frontal cortex of male and female rats.
title_sort comparison of effect of sex hormone manipulation during neonatal period, on mrna expression of slc9a4, nr3c2, htr5b and mas1 in hippocampus and frontal cortex of male and female rats.
url http://psasir.upm.edu.my/id/eprint/29435/
http://psasir.upm.edu.my/id/eprint/29435/1/Comparison%20of%20effect%20of%20sex%20hormone%20manipulation%20during%20neonatal%20period.pdf