Probiotic strains for shellfish aquaculture: protection of eastern oyster, Crassostrea virginica, larvae and juveniles againsl bacterial challenge
Bacterial pathogens, including several Vibrio spp. and Roseovarius crassostreae, cause severe mortality of larval and juvenile eastern oysters. The introduction of beneficial bacterial isolates in oyster hatcheries and nurseries for the biocontrol of bacterial diseases is a good alternative to the u...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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National Shellfisheries Association
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29416/ http://psasir.upm.edu.my/id/eprint/29416/1/Probiotic%20strains%20for%20shellfish%20aquaculture%20protection%20of%20eastern%20oyster%2C%20Crassostrea%20virginica%2C%20larvae%20and%20juveniles%20againsl%20bacterial%20challenge.pdf |
| _version_ | 1848846393960038400 |
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| author | Abd Karim, Murni Marlina Zhao, Wenjing Rowley, David Nelson, David Gomez-Chiarri, Marta |
| author_facet | Abd Karim, Murni Marlina Zhao, Wenjing Rowley, David Nelson, David Gomez-Chiarri, Marta |
| author_sort | Abd Karim, Murni Marlina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bacterial pathogens, including several Vibrio spp. and Roseovarius crassostreae, cause severe mortality of larval and juvenile eastern oysters. The introduction of beneficial bacterial isolates in oyster hatcheries and nurseries for the biocontrol of bacterial diseases is a good alternative to the use of antibiotics. The goal of this study was to screen and characterize marine bacterial isolates as potential agents to prevent larval and juvenile mortality by the oyster pathogens Vibrio tubiashii and R. crassostreae. Screening of bacterial isolates from Rhode Island marine organisms and environment using agar-based assay methods for detection of antimicrobial activity against oyster pathogens led to the isolation of candidate probionts Phaeobacter sp. S4 and Bacillus pumilus RI06-95. Pretreatment of larval and juvenile oysters for 24 h with 102–106 cfu/mL Phaeobacter sp. S4 or B. pumilus RI06-95 protected larval oysters against mortality resulting from challenge with R. crassostreae and V. tubiashii (relative percent survival (RPS) range, 9%–56%). These probiotics also protected juvenile oysters against challenge with V. tubiashii (RPS, 37%–50%). Probiotic isolates had no negative impact on oyster survival. Protection conferred to larvae against bacterial challenge was short-lived, lasting for only 24 h after removal of the probiotics from the incubation water. These results suggest the potential of marine bacterial isolates Phaeobacter sp. S4 and B. pumilus RI06-95 to serve as biocontrol agents to reduce the impact of bacterial pathogens in the culture of Crassostrea virginica. |
| first_indexed | 2025-11-15T09:02:00Z |
| format | Article |
| id | upm-29416 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:00Z |
| publishDate | 2013 |
| publisher | National Shellfisheries Association |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-294162016-07-13T01:42:18Z http://psasir.upm.edu.my/id/eprint/29416/ Probiotic strains for shellfish aquaculture: protection of eastern oyster, Crassostrea virginica, larvae and juveniles againsl bacterial challenge Abd Karim, Murni Marlina Zhao, Wenjing Rowley, David Nelson, David Gomez-Chiarri, Marta Bacterial pathogens, including several Vibrio spp. and Roseovarius crassostreae, cause severe mortality of larval and juvenile eastern oysters. The introduction of beneficial bacterial isolates in oyster hatcheries and nurseries for the biocontrol of bacterial diseases is a good alternative to the use of antibiotics. The goal of this study was to screen and characterize marine bacterial isolates as potential agents to prevent larval and juvenile mortality by the oyster pathogens Vibrio tubiashii and R. crassostreae. Screening of bacterial isolates from Rhode Island marine organisms and environment using agar-based assay methods for detection of antimicrobial activity against oyster pathogens led to the isolation of candidate probionts Phaeobacter sp. S4 and Bacillus pumilus RI06-95. Pretreatment of larval and juvenile oysters for 24 h with 102–106 cfu/mL Phaeobacter sp. S4 or B. pumilus RI06-95 protected larval oysters against mortality resulting from challenge with R. crassostreae and V. tubiashii (relative percent survival (RPS) range, 9%–56%). These probiotics also protected juvenile oysters against challenge with V. tubiashii (RPS, 37%–50%). Probiotic isolates had no negative impact on oyster survival. Protection conferred to larvae against bacterial challenge was short-lived, lasting for only 24 h after removal of the probiotics from the incubation water. These results suggest the potential of marine bacterial isolates Phaeobacter sp. S4 and B. pumilus RI06-95 to serve as biocontrol agents to reduce the impact of bacterial pathogens in the culture of Crassostrea virginica. National Shellfisheries Association 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29416/1/Probiotic%20strains%20for%20shellfish%20aquaculture%20protection%20of%20eastern%20oyster%2C%20Crassostrea%20virginica%2C%20larvae%20and%20juveniles%20againsl%20bacterial%20challenge.pdf Abd Karim, Murni Marlina and Zhao, Wenjing and Rowley, David and Nelson, David and Gomez-Chiarri, Marta (2013) Probiotic strains for shellfish aquaculture: protection of eastern oyster, Crassostrea virginica, larvae and juveniles againsl bacterial challenge. Journal of Shellfish Research, 32 (2). pp. 401-408. ISSN 0730-8000; ESSN: 1943-6319 http://www.bioone.org/doi/abs/10.2983/035.032.0220 10.2983/035.032.0220 |
| spellingShingle | Abd Karim, Murni Marlina Zhao, Wenjing Rowley, David Nelson, David Gomez-Chiarri, Marta Probiotic strains for shellfish aquaculture: protection of eastern oyster, Crassostrea virginica, larvae and juveniles againsl bacterial challenge |
| title | Probiotic strains for shellfish aquaculture: protection of eastern oyster, Crassostrea virginica, larvae and juveniles againsl bacterial challenge |
| title_full | Probiotic strains for shellfish aquaculture: protection of eastern oyster, Crassostrea virginica, larvae and juveniles againsl bacterial challenge |
| title_fullStr | Probiotic strains for shellfish aquaculture: protection of eastern oyster, Crassostrea virginica, larvae and juveniles againsl bacterial challenge |
| title_full_unstemmed | Probiotic strains for shellfish aquaculture: protection of eastern oyster, Crassostrea virginica, larvae and juveniles againsl bacterial challenge |
| title_short | Probiotic strains for shellfish aquaculture: protection of eastern oyster, Crassostrea virginica, larvae and juveniles againsl bacterial challenge |
| title_sort | probiotic strains for shellfish aquaculture: protection of eastern oyster, crassostrea virginica, larvae and juveniles againsl bacterial challenge |
| url | http://psasir.upm.edu.my/id/eprint/29416/ http://psasir.upm.edu.my/id/eprint/29416/ http://psasir.upm.edu.my/id/eprint/29416/ http://psasir.upm.edu.my/id/eprint/29416/1/Probiotic%20strains%20for%20shellfish%20aquaculture%20protection%20of%20eastern%20oyster%2C%20Crassostrea%20virginica%2C%20larvae%20and%20juveniles%20againsl%20bacterial%20challenge.pdf |