Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions
Metallothioneins (MTs) are cysteine-rich metal-binding proteins that are involved in cell growth regulation, transportation of metal ions and detoxification of heavy metals. A mesocarp-specific metallothionein-like gene (MT3-A) promoter was isolated from the oil palm (Elaeis guineensis Jacq). A vect...
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| Format: | Article |
| Language: | English |
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Elsevier
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29414/ http://psasir.upm.edu.my/id/eprint/29414/1/Breaking-off%20tissue%20specific%20activity%20of%20the%20oil%20palm%20metallothionein-like%20gene%20promoter%20in%20T1%20seedlings%20of%20tomato%20exposed%20to%20metal%20ions.pdf |
| _version_ | 1848846393476644864 |
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| author | Kamaladini, Hossein Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Ismail, Ismanizan Haddadi, Fatemeh |
| author_facet | Kamaladini, Hossein Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Ismail, Ismanizan Haddadi, Fatemeh |
| author_sort | Kamaladini, Hossein |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Metallothioneins (MTs) are cysteine-rich metal-binding proteins that are involved in cell growth regulation, transportation of metal ions and detoxification of heavy metals. A mesocarp-specific metallothionein-like gene (MT3-A) promoter was isolated from the oil palm (Elaeis guineensis Jacq). A vector construct containing the MT3-A promoter fused to the β-glucuronidase (GUS) gene in the pCAMBIA 1304 vector was produced and used in Agrobacterium-mediated transformation of tomato. Histochemical GUS assay of different tissues of transgenic tomato showed that the MT3-A promoter only drove GUS expression in the reproductive tissues and organs, including the anther, fruit and seed coat. Competitive RT-PCR and GUS fluorometric assay showed changes in the level of GUS mRNA and enzyme activity in the transgenic tomato (T0). No GUS mRNA was found in roots and leaves of transgenic tomato. In contrast, the leaves of transgenic tomato seedlings (T1) produced the highest GUS activity when treated with 150 μM Cu2+ compared to the control (without Cu2+). However, Zn2+ and Fe2+ treatments did not show GUS expression in the leaves of the transgenic tomato seedlings. Interestingly, the results showed a breaking-off tissue-specific activity of the oil palm MT3-A promoter in T1 seedlings of tomato when subjected to Cu2+ ions. |
| first_indexed | 2025-11-15T09:02:00Z |
| format | Article |
| id | upm-29414 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:02:00Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-294142016-07-13T01:49:51Z http://psasir.upm.edu.my/id/eprint/29414/ Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions Kamaladini, Hossein Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Ismail, Ismanizan Haddadi, Fatemeh Metallothioneins (MTs) are cysteine-rich metal-binding proteins that are involved in cell growth regulation, transportation of metal ions and detoxification of heavy metals. A mesocarp-specific metallothionein-like gene (MT3-A) promoter was isolated from the oil palm (Elaeis guineensis Jacq). A vector construct containing the MT3-A promoter fused to the β-glucuronidase (GUS) gene in the pCAMBIA 1304 vector was produced and used in Agrobacterium-mediated transformation of tomato. Histochemical GUS assay of different tissues of transgenic tomato showed that the MT3-A promoter only drove GUS expression in the reproductive tissues and organs, including the anther, fruit and seed coat. Competitive RT-PCR and GUS fluorometric assay showed changes in the level of GUS mRNA and enzyme activity in the transgenic tomato (T0). No GUS mRNA was found in roots and leaves of transgenic tomato. In contrast, the leaves of transgenic tomato seedlings (T1) produced the highest GUS activity when treated with 150 μM Cu2+ compared to the control (without Cu2+). However, Zn2+ and Fe2+ treatments did not show GUS expression in the leaves of the transgenic tomato seedlings. Interestingly, the results showed a breaking-off tissue-specific activity of the oil palm MT3-A promoter in T1 seedlings of tomato when subjected to Cu2+ ions. Elsevier 2013-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29414/1/Breaking-off%20tissue%20specific%20activity%20of%20the%20oil%20palm%20metallothionein-like%20gene%20promoter%20in%20T1%20seedlings%20of%20tomato%20exposed%20to%20metal%20ions.pdf Kamaladini, Hossein and Abdullah, Siti Nor Akmar and Abdul Aziz, Maheran and Ismail, Ismanizan and Haddadi, Fatemeh (2013) Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions. Journal of Plant Physiology, 170 (3). pp. 346-354. ISSN 0176-1617; ESSN: 1618-1328 http://www.sciencedirect.com/science/article/pii/S0176161712005421 10.1016/j.jplph.2012.10.017 |
| spellingShingle | Kamaladini, Hossein Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Ismail, Ismanizan Haddadi, Fatemeh Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions |
| title | Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions |
| title_full | Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions |
| title_fullStr | Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions |
| title_full_unstemmed | Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions |
| title_short | Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions |
| title_sort | breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in t1 seedlings of tomato exposed to metal ions |
| url | http://psasir.upm.edu.my/id/eprint/29414/ http://psasir.upm.edu.my/id/eprint/29414/ http://psasir.upm.edu.my/id/eprint/29414/ http://psasir.upm.edu.my/id/eprint/29414/1/Breaking-off%20tissue%20specific%20activity%20of%20the%20oil%20palm%20metallothionein-like%20gene%20promoter%20in%20T1%20seedlings%20of%20tomato%20exposed%20to%20metal%20ions.pdf |