Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28
The genus Dendrobium possesses horticultural importance. Dendrobium sonia-28 is an important ornamental orchid in the flower industry. Cryopreservation is a favoured long-term storage method for orchids with propagation problems. Protocorm-like bodies (PLBs) of Dendrobium sonia-28 were cryopreserved...
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| Format: | Article |
| Language: | English |
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Scientific and Technological Research Council of Turkey
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29389/ http://psasir.upm.edu.my/id/eprint/29389/1/Histology%20and%20scanning%20electron%20microscopy%20observations%20of%20cryopreserved%20protocorm-like%20bodies%20of%20Dendrobium%20sonia-28.pdf |
| _version_ | 1848846386544508928 |
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| author | Pobathy, Ranjetta Sinniah, Uma Rani Rathinam, Xavier Subramaniam, Sreeramanan |
| author_facet | Pobathy, Ranjetta Sinniah, Uma Rani Rathinam, Xavier Subramaniam, Sreeramanan |
| author_sort | Pobathy, Ranjetta |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The genus Dendrobium possesses horticultural importance. Dendrobium sonia-28 is an important ornamental orchid in the flower industry. Cryopreservation is a favoured long-term storage method for orchids with propagation problems. Protocorm-like bodies (PLBs) of Dendrobium sonia-28 were cryopreserved using the vitrification technique. Histology and scanning electron microscopy (SEM) observations were conducted on stock, non-cryopreserved (control), and cryopreserved PLBs of Dendrobium sonia-28 to detect cryoinjuries resulting from the vitrification protocol. Histological observations of control PLBs indicated that the preculture, osmoprotection, and dehydration steps were not physically damaging to the PLBs. Histological and SEM analyses of cryopreserved PLBs indicated that the freezing and thawing cycles inflicted damages on the parenchymatic regions of the PLBs. Only embryogenic cells survived the treatment. Scanning electron microscopy studies of the control and cryopreserved PLBs indicated that both osmotic and freezing injuries occurred only in the interior regions of the PLBs. |
| first_indexed | 2025-11-15T09:01:53Z |
| format | Article |
| id | upm-29389 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:01:53Z |
| publishDate | 2013 |
| publisher | Scientific and Technological Research Council of Turkey |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-293892016-07-13T01:44:19Z http://psasir.upm.edu.my/id/eprint/29389/ Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28 Pobathy, Ranjetta Sinniah, Uma Rani Rathinam, Xavier Subramaniam, Sreeramanan The genus Dendrobium possesses horticultural importance. Dendrobium sonia-28 is an important ornamental orchid in the flower industry. Cryopreservation is a favoured long-term storage method for orchids with propagation problems. Protocorm-like bodies (PLBs) of Dendrobium sonia-28 were cryopreserved using the vitrification technique. Histology and scanning electron microscopy (SEM) observations were conducted on stock, non-cryopreserved (control), and cryopreserved PLBs of Dendrobium sonia-28 to detect cryoinjuries resulting from the vitrification protocol. Histological observations of control PLBs indicated that the preculture, osmoprotection, and dehydration steps were not physically damaging to the PLBs. Histological and SEM analyses of cryopreserved PLBs indicated that the freezing and thawing cycles inflicted damages on the parenchymatic regions of the PLBs. Only embryogenic cells survived the treatment. Scanning electron microscopy studies of the control and cryopreserved PLBs indicated that both osmotic and freezing injuries occurred only in the interior regions of the PLBs. Scientific and Technological Research Council of Turkey 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29389/1/Histology%20and%20scanning%20electron%20microscopy%20observations%20of%20cryopreserved%20protocorm-like%20bodies%20of%20Dendrobium%20sonia-28.pdf Pobathy, Ranjetta and Sinniah, Uma Rani and Rathinam, Xavier and Subramaniam, Sreeramanan (2013) Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28. Turkish Journal of Biology, 37 (2). pp. 191-198. ISSN 1300-0152; ESSN: 1303-6092 http://journals.tubitak.gov.tr/biology/abstract.htm?id=13532 10.3906/biy-1206-22 |
| spellingShingle | Pobathy, Ranjetta Sinniah, Uma Rani Rathinam, Xavier Subramaniam, Sreeramanan Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28 |
| title | Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28 |
| title_full | Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28 |
| title_fullStr | Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28 |
| title_full_unstemmed | Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28 |
| title_short | Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28 |
| title_sort | histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of dendrobium sonia-28 |
| url | http://psasir.upm.edu.my/id/eprint/29389/ http://psasir.upm.edu.my/id/eprint/29389/ http://psasir.upm.edu.my/id/eprint/29389/ http://psasir.upm.edu.my/id/eprint/29389/1/Histology%20and%20scanning%20electron%20microscopy%20observations%20of%20cryopreserved%20protocorm-like%20bodies%20of%20Dendrobium%20sonia-28.pdf |