Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification
Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, the genus faces both low germination rates and the risk of producing heterozygous progenies. Cryopreservation is currently the favoured long-term storage method for orchids with propagation problems. Vit...
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| Format: | Article |
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Springer
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29385/ http://psasir.upm.edu.my/id/eprint/29385/1/Catalase%20and%20superoxide%20dismutase%20activities%20and%20the%20total%20protein%20content%20of%20protocorm-like%20bodies%20of%20Dendrobium%20sonia-28%20subjected%20to%20vitrification.pdf |
| _version_ | 1848846385349132288 |
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| author | Poobathy, Ranjetta Sinniah, Uma Rani Xavier, Rathinam Subramaniam, Sreeramanan |
| author_facet | Poobathy, Ranjetta Sinniah, Uma Rani Xavier, Rathinam Subramaniam, Sreeramanan |
| author_sort | Poobathy, Ranjetta |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, the genus faces both low germination rates and the risk of producing heterozygous progenies. Cryopreservation is currently the favoured long-term storage method for orchids with propagation problems. Vitrification, a frequently used cryopreservation technique, involves the application of pretreatments and cryoprotectants to protect and recover explants during and after storage in liquid nitrogen. However, cryopreservation may cause osmotic injuries and toxicity to cryopreserved explants from the use of highly concentrated additives, and cellular injuries from thawing, devitrification and ice formation. Reactive oxygen species (ROS), occurring during dehydration and cryopreservation, may also cause membrane damage. Plants possess efficient antioxidant systems such as the superoxide dismutase (SOD) and catalase (CAT) enzymes to scavenge ROS during low temperature stress. In this study, protocorm-like bodies (PLBs) of Dendrobium sonia-28 were assayed for the total protein content, and both SOD and CAT activities, at each stage of a vitrification exercise to observe for deleterious stages in the protocol. The results indicated that cryopreserved PLBs of Dendrobium sonia-28 underwent excessive post-thawing oxidative stress due to decreased levels of the CAT enzyme at the post-thawing recovery stage, which contributed to the poor survival rates of the cryopreserved PLBs. |
| first_indexed | 2025-11-15T09:01:52Z |
| format | Article |
| id | upm-29385 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:01:52Z |
| publishDate | 2013 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-293852016-07-13T01:43:06Z http://psasir.upm.edu.my/id/eprint/29385/ Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification Poobathy, Ranjetta Sinniah, Uma Rani Xavier, Rathinam Subramaniam, Sreeramanan Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, the genus faces both low germination rates and the risk of producing heterozygous progenies. Cryopreservation is currently the favoured long-term storage method for orchids with propagation problems. Vitrification, a frequently used cryopreservation technique, involves the application of pretreatments and cryoprotectants to protect and recover explants during and after storage in liquid nitrogen. However, cryopreservation may cause osmotic injuries and toxicity to cryopreserved explants from the use of highly concentrated additives, and cellular injuries from thawing, devitrification and ice formation. Reactive oxygen species (ROS), occurring during dehydration and cryopreservation, may also cause membrane damage. Plants possess efficient antioxidant systems such as the superoxide dismutase (SOD) and catalase (CAT) enzymes to scavenge ROS during low temperature stress. In this study, protocorm-like bodies (PLBs) of Dendrobium sonia-28 were assayed for the total protein content, and both SOD and CAT activities, at each stage of a vitrification exercise to observe for deleterious stages in the protocol. The results indicated that cryopreserved PLBs of Dendrobium sonia-28 underwent excessive post-thawing oxidative stress due to decreased levels of the CAT enzyme at the post-thawing recovery stage, which contributed to the poor survival rates of the cryopreserved PLBs. Springer 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29385/1/Catalase%20and%20superoxide%20dismutase%20activities%20and%20the%20total%20protein%20content%20of%20protocorm-like%20bodies%20of%20Dendrobium%20sonia-28%20subjected%20to%20vitrification.pdf Poobathy, Ranjetta and Sinniah, Uma Rani and Xavier, Rathinam and Subramaniam, Sreeramanan (2013) Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification. Applied Biochemistry and Biotechnology, 170 (5). pp. 1066-1079. ISSN 0273-2289; ESSN: 1559-0291 http://link.springer.com/article/10.1007%2Fs12010-013-0241-z 10.1007/s12010-013-0241-z |
| spellingShingle | Poobathy, Ranjetta Sinniah, Uma Rani Xavier, Rathinam Subramaniam, Sreeramanan Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification |
| title | Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification |
| title_full | Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification |
| title_fullStr | Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification |
| title_full_unstemmed | Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification |
| title_short | Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification |
| title_sort | catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of dendrobium sonia-28 subjected to vitrification |
| url | http://psasir.upm.edu.my/id/eprint/29385/ http://psasir.upm.edu.my/id/eprint/29385/ http://psasir.upm.edu.my/id/eprint/29385/ http://psasir.upm.edu.my/id/eprint/29385/1/Catalase%20and%20superoxide%20dismutase%20activities%20and%20the%20total%20protein%20content%20of%20protocorm-like%20bodies%20of%20Dendrobium%20sonia-28%20subjected%20to%20vitrification.pdf |