Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea
Understanding the scale at which wild stocks of Persian sturgeon (Acipenser persicus) are genetically discrete is necessary for effective management of this commercially important species. Disomic DNA microsatellite markers are among the best tools for determining stock structure in fishes. As all s...
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| Format: | Article |
| Language: | English |
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Iranian Fisheries Science Research Institute
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29326/ http://psasir.upm.edu.my/id/eprint/29326/1/Development%20of%20disomic%20single-locus%20DNA%20microsatellite%20markers%20for%20Persian%20sturgeon%20%28Acipenser%20persicus%29%20from%20the%20Caspian%20Sea.pdf |
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| author | Moghim, Mehdi Pourkazemi, Mohammad Tan, Soon Guan Siraj, Siti Shapor Panandam, Jothi Malar Kor, Davod Taghavi, M. J. |
| author_facet | Moghim, Mehdi Pourkazemi, Mohammad Tan, Soon Guan Siraj, Siti Shapor Panandam, Jothi Malar Kor, Davod Taghavi, M. J. |
| author_sort | Moghim, Mehdi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Understanding the scale at which wild stocks of Persian sturgeon (Acipenser persicus) are genetically discrete is necessary for effective management of this commercially important species. Disomic DNA microsatellite markers are among the best tools for determining stock structure in fishes. As all sturgeon species have a polyploid ancestry of all sturgeons, most gene loci exhibit more than two alleles per individual, limiting the use of powerful analytical methods that commonly assume disomic inheritance. We scored products from 38 sets of microsatellite primers developed in lake (Acipenser fulvescens) and Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) to determine whether they would amplify disomic loci in Persian sturgeon. Samples of 45 individuals were detected.Thirty six loci (95%) were amplified successfully in Persian sturgeon. We identified a single monomorphic locus, 12 disomic, 19 tetrasomic, three octosomic, and one locus that was ambiguous. This is the first report on development of disomic single-locus DNA microsatellite markers in Persian sturgeon. These loci could be used to characterize variation in geographically discrete populations of the Persian sturgeon in their native ecosystem including in the Caspian Sea. |
| first_indexed | 2025-11-15T09:01:36Z |
| format | Article |
| id | upm-29326 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:01:36Z |
| publishDate | 2013 |
| publisher | Iranian Fisheries Science Research Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-293262016-07-13T02:11:07Z http://psasir.upm.edu.my/id/eprint/29326/ Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea Moghim, Mehdi Pourkazemi, Mohammad Tan, Soon Guan Siraj, Siti Shapor Panandam, Jothi Malar Kor, Davod Taghavi, M. J. Understanding the scale at which wild stocks of Persian sturgeon (Acipenser persicus) are genetically discrete is necessary for effective management of this commercially important species. Disomic DNA microsatellite markers are among the best tools for determining stock structure in fishes. As all sturgeon species have a polyploid ancestry of all sturgeons, most gene loci exhibit more than two alleles per individual, limiting the use of powerful analytical methods that commonly assume disomic inheritance. We scored products from 38 sets of microsatellite primers developed in lake (Acipenser fulvescens) and Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) to determine whether they would amplify disomic loci in Persian sturgeon. Samples of 45 individuals were detected.Thirty six loci (95%) were amplified successfully in Persian sturgeon. We identified a single monomorphic locus, 12 disomic, 19 tetrasomic, three octosomic, and one locus that was ambiguous. This is the first report on development of disomic single-locus DNA microsatellite markers in Persian sturgeon. These loci could be used to characterize variation in geographically discrete populations of the Persian sturgeon in their native ecosystem including in the Caspian Sea. Iranian Fisheries Science Research Institute 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29326/1/Development%20of%20disomic%20single-locus%20DNA%20microsatellite%20markers%20for%20Persian%20sturgeon%20%28Acipenser%20persicus%29%20from%20the%20Caspian%20Sea.pdf Moghim, Mehdi and Pourkazemi, Mohammad and Tan, Soon Guan and Siraj, Siti Shapor and Panandam, Jothi Malar and Kor, Davod and Taghavi, M. J. (2013) Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea. Iranian Journal of Fisheries Sciences, 12 (2). pp. 389-397. ISSN 1562-2916 http://www.jifro.ir/browse.php?a_code=A-10-348-3&slc_lang=en&sid=1 |
| spellingShingle | Moghim, Mehdi Pourkazemi, Mohammad Tan, Soon Guan Siraj, Siti Shapor Panandam, Jothi Malar Kor, Davod Taghavi, M. J. Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea |
| title | Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea |
| title_full | Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea |
| title_fullStr | Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea |
| title_full_unstemmed | Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea |
| title_short | Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea |
| title_sort | development of disomic single-locus dna microsatellite markers for persian sturgeon (acipenser persicus) from the caspian sea |
| url | http://psasir.upm.edu.my/id/eprint/29326/ http://psasir.upm.edu.my/id/eprint/29326/ http://psasir.upm.edu.my/id/eprint/29326/1/Development%20of%20disomic%20single-locus%20DNA%20microsatellite%20markers%20for%20Persian%20sturgeon%20%28Acipenser%20persicus%29%20from%20the%20Caspian%20Sea.pdf |