Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.)
The biochemical basis of resistance to the acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicide diclofop-methyl was investigated in a resistant wild oat population (R1), which does not exhibit a resistant ACCase. Rates of foliar uptake and translocation of [14C]-diclofop were the same in the...
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| Format: | Article |
| Language: | English |
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Weed Science Society of America
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29322/ http://psasir.upm.edu.my/id/eprint/29322/1/Herbicide%20resistance%20endowed%20by%20enhanced%20rates%20of%20herbicide%20metabolism%20in%20wild%20oat%20%28Avena%20spp.%29.pdf |
| _version_ | 1848846367715229696 |
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| author | Ahmad Hamdani, Muhammad Saiful Yu, Qin Han, Heping Cawthray, Gregory R. Wang, Shaofang Powles, Stephen B. |
| author_facet | Ahmad Hamdani, Muhammad Saiful Yu, Qin Han, Heping Cawthray, Gregory R. Wang, Shaofang Powles, Stephen B. |
| author_sort | Ahmad Hamdani, Muhammad Saiful |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The biochemical basis of resistance to the acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicide diclofop-methyl was investigated in a resistant wild oat population (R1), which does not exhibit a resistant ACCase. Rates of foliar uptake and translocation of [14C]-diclofop were the same in the R1 vs. susceptible (S) populations. However, the level of phytotoxic diclofop acid was always found to be lower in the R1 vs. S plants, with a concomitant higher level (up to 1.7-fold) of nontoxic polar diclofop metabolites in R1 relative to the S plants. These results indicate that a non–target-site-based mechanism of enhanced rate of diclofop acid metabolism confers resistance in population R1. Moreover, the high-performance liquid chromotography elution profile of the major diclofop metabolites in R1 is similar to that of wheat, suggesting resistance in individuals of population R1 involves a wheat-like detoxification system mediated by cytochrome P450 monooxygenases. In addition, lower level of tissue diclofop acid was also observed using nonradioactive ultra-performance liquid chromatography–mass spectrometry analysis in resistant individuals of three other resistant wild oat populations (R2, R3, and R4) known to posses ACCase gene resistance mutations. These results establish that either one or at least two independent resistance mechanisms (target-site ACCase resistance mutations and non–target-site enhanced rates of herbicide metabolism) can be present in individual wild oat plants. |
| first_indexed | 2025-11-15T09:01:35Z |
| format | Article |
| id | upm-29322 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:01:35Z |
| publishDate | 2013 |
| publisher | Weed Science Society of America |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-293222016-07-13T03:24:39Z http://psasir.upm.edu.my/id/eprint/29322/ Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.) Ahmad Hamdani, Muhammad Saiful Yu, Qin Han, Heping Cawthray, Gregory R. Wang, Shaofang Powles, Stephen B. The biochemical basis of resistance to the acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicide diclofop-methyl was investigated in a resistant wild oat population (R1), which does not exhibit a resistant ACCase. Rates of foliar uptake and translocation of [14C]-diclofop were the same in the R1 vs. susceptible (S) populations. However, the level of phytotoxic diclofop acid was always found to be lower in the R1 vs. S plants, with a concomitant higher level (up to 1.7-fold) of nontoxic polar diclofop metabolites in R1 relative to the S plants. These results indicate that a non–target-site-based mechanism of enhanced rate of diclofop acid metabolism confers resistance in population R1. Moreover, the high-performance liquid chromotography elution profile of the major diclofop metabolites in R1 is similar to that of wheat, suggesting resistance in individuals of population R1 involves a wheat-like detoxification system mediated by cytochrome P450 monooxygenases. In addition, lower level of tissue diclofop acid was also observed using nonradioactive ultra-performance liquid chromatography–mass spectrometry analysis in resistant individuals of three other resistant wild oat populations (R2, R3, and R4) known to posses ACCase gene resistance mutations. These results establish that either one or at least two independent resistance mechanisms (target-site ACCase resistance mutations and non–target-site enhanced rates of herbicide metabolism) can be present in individual wild oat plants. Weed Science Society of America 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29322/1/Herbicide%20resistance%20endowed%20by%20enhanced%20rates%20of%20herbicide%20metabolism%20in%20wild%20oat%20%28Avena%20spp.%29.pdf Ahmad Hamdani, Muhammad Saiful and Yu, Qin and Han, Heping and Cawthray, Gregory R. and Wang, Shaofang and Powles, Stephen B. (2013) Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.). Weed Science, 61 (1). pp. 55-62. ISSN 0043-1745; ESSN: 1550-2759 http://www.bioone.org/doi/abs/10.1614/WS-D-12-00078.1 10.1614/WS-D-12-00078.1 |
| spellingShingle | Ahmad Hamdani, Muhammad Saiful Yu, Qin Han, Heping Cawthray, Gregory R. Wang, Shaofang Powles, Stephen B. Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.) |
| title | Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.) |
| title_full | Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.) |
| title_fullStr | Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.) |
| title_full_unstemmed | Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.) |
| title_short | Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.) |
| title_sort | herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (avena spp.) |
| url | http://psasir.upm.edu.my/id/eprint/29322/ http://psasir.upm.edu.my/id/eprint/29322/ http://psasir.upm.edu.my/id/eprint/29322/ http://psasir.upm.edu.my/id/eprint/29322/1/Herbicide%20resistance%20endowed%20by%20enhanced%20rates%20of%20herbicide%20metabolism%20in%20wild%20oat%20%28Avena%20spp.%29.pdf |