Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing
The objective of this study was to isolate and identify novel potential probiotic Lactobacillus using a culture method and Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene. Seventeen Lactobacillus strains were isolated from a full honey comb of the honey bee Apis dorsata using sele...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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International Bee Research Association
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29319/ http://psasir.upm.edu.my/id/eprint/29319/1/Molecular%20identification%20of%20Lactobacillus%20spp.%20isolated%20from%20the%20honey%20comb%20of%20the%20honey%20bee%20%28Apis%20dorsata%29%20by%2016S%20rRNA%20gene%20sequencing.pdf |
| _version_ | 1848846366718033920 |
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| author | Tajabadi, Naser Mardan, Makhdzir Abd Manap, Mohd Yazid Mustafa, Shuhaimi |
| author_facet | Tajabadi, Naser Mardan, Makhdzir Abd Manap, Mohd Yazid Mustafa, Shuhaimi |
| author_sort | Tajabadi, Naser |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The objective of this study was to isolate and identify novel potential probiotic Lactobacillus using a culture method and Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene. Seventeen Lactobacillus strains were isolated from a full honey comb of the honey bee Apis dorsata using selective media. The 16S rRNA genes from the extracted DNA of bacterial colonies were amplified with PCR using universal bacteria primers. All bacterial 16S rRNA genes were sequenced, aligned and the distant bacteria were deposited in GenBank. The lactobacilli strains were identified as Lactobacillus spp. related-sequences (64.15%) with other abundant sequences being related to Lactobacillus kunkeei (34.85%). The findings revealed that Apis dorsata honey comb has potential to be a source of new bacteria with probiotic activities in honey bee or as natural food preservatives for the food industry. |
| first_indexed | 2025-11-15T09:01:34Z |
| format | Article |
| id | upm-29319 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:01:34Z |
| publishDate | 2013 |
| publisher | International Bee Research Association |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-293192016-07-13T02:26:29Z http://psasir.upm.edu.my/id/eprint/29319/ Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing Tajabadi, Naser Mardan, Makhdzir Abd Manap, Mohd Yazid Mustafa, Shuhaimi The objective of this study was to isolate and identify novel potential probiotic Lactobacillus using a culture method and Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene. Seventeen Lactobacillus strains were isolated from a full honey comb of the honey bee Apis dorsata using selective media. The 16S rRNA genes from the extracted DNA of bacterial colonies were amplified with PCR using universal bacteria primers. All bacterial 16S rRNA genes were sequenced, aligned and the distant bacteria were deposited in GenBank. The lactobacilli strains were identified as Lactobacillus spp. related-sequences (64.15%) with other abundant sequences being related to Lactobacillus kunkeei (34.85%). The findings revealed that Apis dorsata honey comb has potential to be a source of new bacteria with probiotic activities in honey bee or as natural food preservatives for the food industry. International Bee Research Association 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29319/1/Molecular%20identification%20of%20Lactobacillus%20spp.%20isolated%20from%20the%20honey%20comb%20of%20the%20honey%20bee%20%28Apis%20dorsata%29%20by%2016S%20rRNA%20gene%20sequencing.pdf Tajabadi, Naser and Mardan, Makhdzir and Abd Manap, Mohd Yazid and Mustafa, Shuhaimi (2013) Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing. Journal of Apicultural Research, 52 (5). pp. 235-241. ISSN 0021-8839; ESSN: 2078-6913 http://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.5.10?journalCode=tjar20 10.3896/IBRA.1.52.5.10 |
| spellingShingle | Tajabadi, Naser Mardan, Makhdzir Abd Manap, Mohd Yazid Mustafa, Shuhaimi Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing |
| title | Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing |
| title_full | Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing |
| title_fullStr | Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing |
| title_full_unstemmed | Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing |
| title_short | Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16S rRNA gene sequencing |
| title_sort | molecular identification of lactobacillus spp. isolated from the honey comb of the honey bee (apis dorsata) by 16s rrna gene sequencing |
| url | http://psasir.upm.edu.my/id/eprint/29319/ http://psasir.upm.edu.my/id/eprint/29319/ http://psasir.upm.edu.my/id/eprint/29319/ http://psasir.upm.edu.my/id/eprint/29319/1/Molecular%20identification%20of%20Lactobacillus%20spp.%20isolated%20from%20the%20honey%20comb%20of%20the%20honey%20bee%20%28Apis%20dorsata%29%20by%2016S%20rRNA%20gene%20sequencing.pdf |