Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds
Bakanae is one of the important diseases of rice (Oryzae sativa L.). To evaluate the pathogen(s) responsible for bakanae disease of rice in Malaysia, 12 isolates of Fusarium spp. were obtained from infected rice plants samples from rice growing in Tanjung Karang and Sekinchan areas of Selangor. All...
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| Format: | Article |
| Language: | English |
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Friends Science Publishers
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29310/ http://psasir.upm.edu.my/id/eprint/29310/1/Characterization%20of%20Fusarium%20proliferatum%20through%20species%20specific%20primers%20and%20its%20virulence%20on%20rice%20seeds.pdf |
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| author | Jahan, Quazi Shireen Akhter Meon, Sariah Jaafar, Hawa Z. E. Mior Ahmad, Zainal Abidin |
| author_facet | Jahan, Quazi Shireen Akhter Meon, Sariah Jaafar, Hawa Z. E. Mior Ahmad, Zainal Abidin |
| author_sort | Jahan, Quazi Shireen Akhter |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bakanae is one of the important diseases of rice (Oryzae sativa L.). To evaluate the pathogen(s) responsible for bakanae disease of rice in Malaysia, 12 isolates of Fusarium spp. were obtained from infected rice plants samples from rice growing in Tanjung Karang and Sekinchan areas of Selangor. All isolates were identified as F. proliferatum based on morphological characteristics and confirmed by amplification of DNA with species specific primer pairs Pro 1/2 at 554 bp. The rDNA-ITS primer sequences showed 99% homology with F. proliferatum isolâtes AJ810449.1, X94171.1, GU363955.1, EU151488.1, HQ380789.1, HQ332533.1, GU594758.1 and EU03930366.1. Pathogenicity testing on susceptible rice variety MR 211 proved all isolates to be pathogenic based on increase in plant height (%) decrease in main root length (%) and decrease in lateral roots number (%) of inoculated plants compared to control plants. |
| first_indexed | 2025-11-15T09:01:32Z |
| format | Article |
| id | upm-29310 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:01:32Z |
| publishDate | 2013 |
| publisher | Friends Science Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-293102016-07-13T04:47:04Z http://psasir.upm.edu.my/id/eprint/29310/ Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds Jahan, Quazi Shireen Akhter Meon, Sariah Jaafar, Hawa Z. E. Mior Ahmad, Zainal Abidin Bakanae is one of the important diseases of rice (Oryzae sativa L.). To evaluate the pathogen(s) responsible for bakanae disease of rice in Malaysia, 12 isolates of Fusarium spp. were obtained from infected rice plants samples from rice growing in Tanjung Karang and Sekinchan areas of Selangor. All isolates were identified as F. proliferatum based on morphological characteristics and confirmed by amplification of DNA with species specific primer pairs Pro 1/2 at 554 bp. The rDNA-ITS primer sequences showed 99% homology with F. proliferatum isolâtes AJ810449.1, X94171.1, GU363955.1, EU151488.1, HQ380789.1, HQ332533.1, GU594758.1 and EU03930366.1. Pathogenicity testing on susceptible rice variety MR 211 proved all isolates to be pathogenic based on increase in plant height (%) decrease in main root length (%) and decrease in lateral roots number (%) of inoculated plants compared to control plants. Friends Science Publishers 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29310/1/Characterization%20of%20Fusarium%20proliferatum%20through%20species%20specific%20primers%20and%20its%20virulence%20on%20rice%20seeds.pdf Jahan, Quazi Shireen Akhter and Meon, Sariah and Jaafar, Hawa Z. E. and Mior Ahmad, Zainal Abidin (2013) Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds. International Journal of Agriculture and Biology, 15 (4). pp. 649-656. ISSN 1560-8530; ESSN: 1814–9596 http://www.fspublishers.org/Issue.php?categoryID=62 |
| spellingShingle | Jahan, Quazi Shireen Akhter Meon, Sariah Jaafar, Hawa Z. E. Mior Ahmad, Zainal Abidin Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds |
| title | Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds |
| title_full | Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds |
| title_fullStr | Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds |
| title_full_unstemmed | Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds |
| title_short | Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds |
| title_sort | characterization of fusarium proliferatum through species specific primers and its virulence on rice seeds |
| url | http://psasir.upm.edu.my/id/eprint/29310/ http://psasir.upm.edu.my/id/eprint/29310/ http://psasir.upm.edu.my/id/eprint/29310/1/Characterization%20of%20Fusarium%20proliferatum%20through%20species%20specific%20primers%20and%20its%20virulence%20on%20rice%20seeds.pdf |