Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker

The knowledge of genetic diversity of tree crop is very important for breeding and improvement program for the purpose of improving the yield and quality of its produce. Genetic diversity study and analysis of genetic relationship among 20 Moringa oleifera were carried out with the aid of twelve pri...

Full description

Bibliographic Details
Main Authors: Rufai, Shamsuddeen, Musa, Mohamed Hanafi, Yusop, Mohd Rafii, Selamat, Ahmad, Arolu, Ibrahim Wasiu, Ferdous, Jannatul
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29272/
http://psasir.upm.edu.my/id/eprint/29272/1/Genetic%20Dissection%20of%20New%20Genotypes%20of%20Drumstick%20Tree%20%28Moringa%20oleifera%20Lam.%29%20Using%20Random%20Amplified%20Polymorphic%20DNA%20Marker.pdf
_version_ 1848846353218666496
author Rufai, Shamsuddeen
Musa, Mohamed Hanafi
Yusop, Mohd Rafii
Selamat, Ahmad
Arolu, Ibrahim Wasiu
Ferdous, Jannatul
author_facet Rufai, Shamsuddeen
Musa, Mohamed Hanafi
Yusop, Mohd Rafii
Selamat, Ahmad
Arolu, Ibrahim Wasiu
Ferdous, Jannatul
author_sort Rufai, Shamsuddeen
building UPM Institutional Repository
collection Online Access
description The knowledge of genetic diversity of tree crop is very important for breeding and improvement program for the purpose of improving the yield and quality of its produce. Genetic diversity study and analysis of genetic relationship among 20 Moringa oleifera were carried out with the aid of twelve primers from, random amplified polymorphic DNA marker. The seeds of twenty M. oleifera genotypes from various origins were collected and germinated and raised in nursery before transplanting to the field at University Agricultural Park (TPU). Genetic diversity parameter, such as Shannon's information index and expected heterozygosity, revealed the presence of high genetic divergence with value of 1.80 and 0.13 for Malaysian population and 0.30 and 0.19 for the international population, respectively. Mean of Nei's gene diversity index for the two populations was estimated to be 0.20. In addition, a dendrogram constructed, using UPGMA cluster analysis based on Nei's genetic distance, grouped the twenty M. oleifera into five distinct clusters. The study revealed a great extent of variation which is essential for successful breeding and improvement program. From this study, M. oleifera genotypes of wide genetic origin, such as T-01, T-06, M-01, and M-02, are recommended to be used as parent in future breeding program.
first_indexed 2025-11-15T09:01:21Z
format Article
id upm-29272
institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T09:01:21Z
publishDate 2013
publisher Hindawi Publishing Corporation
recordtype eprints
repository_type Digital Repository
spelling upm-292722016-04-25T04:43:01Z http://psasir.upm.edu.my/id/eprint/29272/ Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker Rufai, Shamsuddeen Musa, Mohamed Hanafi Yusop, Mohd Rafii Selamat, Ahmad Arolu, Ibrahim Wasiu Ferdous, Jannatul The knowledge of genetic diversity of tree crop is very important for breeding and improvement program for the purpose of improving the yield and quality of its produce. Genetic diversity study and analysis of genetic relationship among 20 Moringa oleifera were carried out with the aid of twelve primers from, random amplified polymorphic DNA marker. The seeds of twenty M. oleifera genotypes from various origins were collected and germinated and raised in nursery before transplanting to the field at University Agricultural Park (TPU). Genetic diversity parameter, such as Shannon's information index and expected heterozygosity, revealed the presence of high genetic divergence with value of 1.80 and 0.13 for Malaysian population and 0.30 and 0.19 for the international population, respectively. Mean of Nei's gene diversity index for the two populations was estimated to be 0.20. In addition, a dendrogram constructed, using UPGMA cluster analysis based on Nei's genetic distance, grouped the twenty M. oleifera into five distinct clusters. The study revealed a great extent of variation which is essential for successful breeding and improvement program. From this study, M. oleifera genotypes of wide genetic origin, such as T-01, T-06, M-01, and M-02, are recommended to be used as parent in future breeding program. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29272/1/Genetic%20Dissection%20of%20New%20Genotypes%20of%20Drumstick%20Tree%20%28Moringa%20oleifera%20Lam.%29%20Using%20Random%20Amplified%20Polymorphic%20DNA%20Marker.pdf Rufai, Shamsuddeen and Musa, Mohamed Hanafi and Yusop, Mohd Rafii and Selamat, Ahmad and Arolu, Ibrahim Wasiu and Ferdous, Jannatul (2013) Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker. BioMed Research International, 2013. art. no. 604598. pp. 1-6. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2013/604598/abs/ 10.1155/2013/604598
spellingShingle Rufai, Shamsuddeen
Musa, Mohamed Hanafi
Yusop, Mohd Rafii
Selamat, Ahmad
Arolu, Ibrahim Wasiu
Ferdous, Jannatul
Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker
title Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker
title_full Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker
title_fullStr Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker
title_full_unstemmed Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker
title_short Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker
title_sort genetic dissection of new genotypes of drumstick tree (moringa oleifera lam.) using random amplified polymorphic dna marker
url http://psasir.upm.edu.my/id/eprint/29272/
http://psasir.upm.edu.my/id/eprint/29272/
http://psasir.upm.edu.my/id/eprint/29272/
http://psasir.upm.edu.my/id/eprint/29272/1/Genetic%20Dissection%20of%20New%20Genotypes%20of%20Drumstick%20Tree%20%28Moringa%20oleifera%20Lam.%29%20Using%20Random%20Amplified%20Polymorphic%20DNA%20Marker.pdf