Genetic divergence of Malaysian upland rices revealed by microsatellite markers

Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. A total of 50 upland rice accessions, 24 from Peninsular Malaysia and 26 from West Malaysia (Sabah state) were investigated using 10 microsatellite (SSR) markers distributed across the rice genome...

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Main Authors: Sohrabi, Mehdi, Yusop, Mohd Rafii, Musa, Mohamed Hanafi, Latif, Mohammad Abdul
Format: Article
Language:English
Published: Southern Cross Publishing 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29271/
http://psasir.upm.edu.my/id/eprint/29271/1/Genetic%20divergence%20of%20Malaysian%20upland%20rices%20revealed%20by%20microsatellite%20markers.pdf
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author Sohrabi, Mehdi
Yusop, Mohd Rafii
Musa, Mohamed Hanafi
Latif, Mohammad Abdul
author_facet Sohrabi, Mehdi
Yusop, Mohd Rafii
Musa, Mohamed Hanafi
Latif, Mohammad Abdul
author_sort Sohrabi, Mehdi
building UPM Institutional Repository
collection Online Access
description Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. A total of 50 upland rice accessions, 24 from Peninsular Malaysia and 26 from West Malaysia (Sabah state) were investigated using 10 microsatellite (SSR) markers distributed across the rice genome to study the genetic diversity. A total of 49 alleles were detected across the 50 accessions. The number of alleles per locus ranged from 2 to 11 with an average of 4.9. The average Polymorphism Information Content (PIC) value was 0.710. A dendrogram was constructed using Jaccard’s similarity coefficient, and accessions were clustered into 7 groups. The most of the accessions were clustered according to their geographical origin. Shannon’s information index ranged from 0.5269 to 2.0050. Nei's gene diversity (h) ranged from 0.3432 to 0.8273. Overall gene flow was 0.0011. In order to develop suitable upland rice varieties, accessions 03838, 03835, 07537, 07538, 03826, 07574, 07588, 07585, 07540, 07575, 07541, 07543, 07544, 07576, 07571, 07539, 03825, 03830 could be used as parents for future breeding program in Malaysian environment.
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institution Universiti Putra Malaysia
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spelling upm-292712016-06-20T08:09:23Z http://psasir.upm.edu.my/id/eprint/29271/ Genetic divergence of Malaysian upland rices revealed by microsatellite markers Sohrabi, Mehdi Yusop, Mohd Rafii Musa, Mohamed Hanafi Latif, Mohammad Abdul Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. A total of 50 upland rice accessions, 24 from Peninsular Malaysia and 26 from West Malaysia (Sabah state) were investigated using 10 microsatellite (SSR) markers distributed across the rice genome to study the genetic diversity. A total of 49 alleles were detected across the 50 accessions. The number of alleles per locus ranged from 2 to 11 with an average of 4.9. The average Polymorphism Information Content (PIC) value was 0.710. A dendrogram was constructed using Jaccard’s similarity coefficient, and accessions were clustered into 7 groups. The most of the accessions were clustered according to their geographical origin. Shannon’s information index ranged from 0.5269 to 2.0050. Nei's gene diversity (h) ranged from 0.3432 to 0.8273. Overall gene flow was 0.0011. In order to develop suitable upland rice varieties, accessions 03838, 03835, 07537, 07538, 03826, 07574, 07588, 07585, 07540, 07575, 07541, 07543, 07544, 07576, 07571, 07539, 03825, 03830 could be used as parents for future breeding program in Malaysian environment. Southern Cross Publishing 2013-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29271/1/Genetic%20divergence%20of%20Malaysian%20upland%20rices%20revealed%20by%20microsatellite%20markers.pdf Sohrabi, Mehdi and Yusop, Mohd Rafii and Musa, Mohamed Hanafi and Latif, Mohammad Abdul (2013) Genetic divergence of Malaysian upland rices revealed by microsatellite markers. Plant Omics, 6 (3). pp. 175-182. ISSN 1836-0661; ESSN: 1836-3644 http://www.pomics.com/may2013.html
spellingShingle Sohrabi, Mehdi
Yusop, Mohd Rafii
Musa, Mohamed Hanafi
Latif, Mohammad Abdul
Genetic divergence of Malaysian upland rices revealed by microsatellite markers
title Genetic divergence of Malaysian upland rices revealed by microsatellite markers
title_full Genetic divergence of Malaysian upland rices revealed by microsatellite markers
title_fullStr Genetic divergence of Malaysian upland rices revealed by microsatellite markers
title_full_unstemmed Genetic divergence of Malaysian upland rices revealed by microsatellite markers
title_short Genetic divergence of Malaysian upland rices revealed by microsatellite markers
title_sort genetic divergence of malaysian upland rices revealed by microsatellite markers
url http://psasir.upm.edu.my/id/eprint/29271/
http://psasir.upm.edu.my/id/eprint/29271/
http://psasir.upm.edu.my/id/eprint/29271/1/Genetic%20divergence%20of%20Malaysian%20upland%20rices%20revealed%20by%20microsatellite%20markers.pdf