Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment
The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpa...
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| Format: | Article |
| Language: | English |
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Gadjah Mada University
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29240/ http://psasir.upm.edu.my/id/eprint/29240/1/29240.pdf |
| _version_ | 1848846344357150720 |
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| author | Syahputra, Bambang Surya Adji Sinniah, Uma Rani Syed Rastan, Syed Omar Ismail, Mohd Razi |
| author_facet | Syahputra, Bambang Surya Adji Sinniah, Uma Rani Syed Rastan, Syed Omar Ismail, Mohd Razi |
| author_sort | Syahputra, Bambang Surya Adji |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpak C18 (150 mm x 4.6 mm i.d; 5 µm) at 30 ± 1° C using mobile phase of acetonitrile-water (30:70%; v/v), pH 6.80. The treatment of PBZ with different concentration of 100, 200, 400, and 600 mg/L reduced the concentration of GA3 in paddy. The level of GA3 in paddy treated with 100 mg/L of PBZ did not show significant difference from untreated one. However, the level of GA3 in paddy treated with other concentrations (200, 400, and 600 mg/L) of PBZ was significantly different (P < 0.05) from untreated paddy. |
| first_indexed | 2025-11-15T09:01:13Z |
| format | Article |
| id | upm-29240 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:01:13Z |
| publishDate | 2013 |
| publisher | Gadjah Mada University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-292402016-04-26T01:38:37Z http://psasir.upm.edu.my/id/eprint/29240/ Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment Syahputra, Bambang Surya Adji Sinniah, Uma Rani Syed Rastan, Syed Omar Ismail, Mohd Razi The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpak C18 (150 mm x 4.6 mm i.d; 5 µm) at 30 ± 1° C using mobile phase of acetonitrile-water (30:70%; v/v), pH 6.80. The treatment of PBZ with different concentration of 100, 200, 400, and 600 mg/L reduced the concentration of GA3 in paddy. The level of GA3 in paddy treated with 100 mg/L of PBZ did not show significant difference from untreated one. However, the level of GA3 in paddy treated with other concentrations (200, 400, and 600 mg/L) of PBZ was significantly different (P < 0.05) from untreated paddy. Gadjah Mada University 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29240/1/29240.pdf Syahputra, Bambang Surya Adji and Sinniah, Uma Rani and Syed Rastan, Syed Omar and Ismail, Mohd Razi (2013) Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment. Journal of Food and Pharmaceutical Sciences, 1 (1). pp. 14-17. ISSN 2089-7200; ESSN: 2339-0948 http://journal.ugm.ac.id/jfps/article/view/1841 |
| spellingShingle | Syahputra, Bambang Surya Adji Sinniah, Uma Rani Syed Rastan, Syed Omar Ismail, Mohd Razi Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment |
| title | Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment |
| title_full | Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment |
| title_fullStr | Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment |
| title_full_unstemmed | Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment |
| title_short | Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment |
| title_sort | changes in gibberellic acid (ga3) content in oryza sativa due to paclobutrazol treatment |
| url | http://psasir.upm.edu.my/id/eprint/29240/ http://psasir.upm.edu.my/id/eprint/29240/ http://psasir.upm.edu.my/id/eprint/29240/1/29240.pdf |