Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology
Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 sample...
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| Format: | Article |
| Language: | English |
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Springer
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29127/ http://psasir.upm.edu.my/id/eprint/29127/1/29127.pdf |
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| author | Tomizawa, Yuki Shinmura, Yoshimi Wee, Alison K. S. Takayama, Koji Asakawa, Takeshi Yllano, Orlex Baylen Salmo III, Severino Garengo Ardli, Erwin Riyanto Tung, Nguyen Xuan Malekal, Norhaslinda Onrizal, Meenakshisundaram, Sankararamasubramanian Halasya Sungkaew, Sarawood Saleh @ Japri, Mohd Nazre Adjie, Bayu Soe, Khin Khin Oguri, Emiko Murakami, Noriaki Watano, Yasuyuki Baba, Shigeyuki Webb, Edward L. Kajita, Tadashi |
| author_facet | Tomizawa, Yuki Shinmura, Yoshimi Wee, Alison K. S. Takayama, Koji Asakawa, Takeshi Yllano, Orlex Baylen Salmo III, Severino Garengo Ardli, Erwin Riyanto Tung, Nguyen Xuan Malekal, Norhaslinda Onrizal, Meenakshisundaram, Sankararamasubramanian Halasya Sungkaew, Sarawood Saleh @ Japri, Mohd Nazre Adjie, Bayu Soe, Khin Khin Oguri, Emiko Murakami, Noriaki Watano, Yasuyuki Baba, Shigeyuki Webb, Edward L. Kajita, Tadashi |
| author_sort | Tomizawa, Yuki |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 samples of widespread mangrove species Xylocarpus granatum (Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range. |
| first_indexed | 2025-11-15T09:00:42Z |
| format | Article |
| id | upm-29127 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:00:42Z |
| publishDate | 2013 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-291272016-04-25T02:46:56Z http://psasir.upm.edu.my/id/eprint/29127/ Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology Tomizawa, Yuki Shinmura, Yoshimi Wee, Alison K. S. Takayama, Koji Asakawa, Takeshi Yllano, Orlex Baylen Salmo III, Severino Garengo Ardli, Erwin Riyanto Tung, Nguyen Xuan Malekal, Norhaslinda Onrizal, Meenakshisundaram, Sankararamasubramanian Halasya Sungkaew, Sarawood Saleh @ Japri, Mohd Nazre Adjie, Bayu Soe, Khin Khin Oguri, Emiko Murakami, Noriaki Watano, Yasuyuki Baba, Shigeyuki Webb, Edward L. Kajita, Tadashi Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 samples of widespread mangrove species Xylocarpus granatum (Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range. Springer 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29127/1/29127.pdf Tomizawa, Yuki and Shinmura, Yoshimi and Wee, Alison K. S. and Takayama, Koji and Asakawa, Takeshi and Yllano, Orlex Baylen and Salmo III, Severino Garengo and Ardli, Erwin Riyanto and Tung, Nguyen Xuan and Malekal, Norhaslinda and Onrizal, and Meenakshisundaram, Sankararamasubramanian Halasya and Sungkaew, Sarawood and Saleh @ Japri, Mohd Nazre and Adjie, Bayu and Soe, Khin Khin and Oguri, Emiko and Murakami, Noriaki and Watano, Yasuyuki and Baba, Shigeyuki and Webb, Edward L. and Kajita, Tadashi (2013) Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology. Conservation Genetics Resources, 5 (4). pp. 1159-1162. ISSN 1877-7252; ESSN: 1877-7260 http://link.springer.com/article/10.1007%2Fs12686-013-9990-9#/page-1 10.1007/s12686-013-9990-9 |
| spellingShingle | Tomizawa, Yuki Shinmura, Yoshimi Wee, Alison K. S. Takayama, Koji Asakawa, Takeshi Yllano, Orlex Baylen Salmo III, Severino Garengo Ardli, Erwin Riyanto Tung, Nguyen Xuan Malekal, Norhaslinda Onrizal, Meenakshisundaram, Sankararamasubramanian Halasya Sungkaew, Sarawood Saleh @ Japri, Mohd Nazre Adjie, Bayu Soe, Khin Khin Oguri, Emiko Murakami, Noriaki Watano, Yasuyuki Baba, Shigeyuki Webb, Edward L. Kajita, Tadashi Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology |
| title | Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology |
| title_full | Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology |
| title_fullStr | Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology |
| title_full_unstemmed | Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology |
| title_short | Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology |
| title_sort | development of 11 polymorphic microsatellite markers for xylocarpus granatum (meliaceae) using next-generation sequencing technology |
| url | http://psasir.upm.edu.my/id/eprint/29127/ http://psasir.upm.edu.my/id/eprint/29127/ http://psasir.upm.edu.my/id/eprint/29127/ http://psasir.upm.edu.my/id/eprint/29127/1/29127.pdf |