An on-line sample pretreatment technique for the HPLC analysis of plant samples.
A continuous-flow, on-line sample pretreatment technique using a silica gel microsyringe extractor has been developed. All steps including extraction, separation, clean-up, and concentration occur in the microsyringe. The overall sample pretreatment process takes <10 min per sample. Different pol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Wiley
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28936/ http://psasir.upm.edu.my/id/eprint/28936/1/An%20on-line%20sample%20pretreatment%20technique%20for%20the%20HPLC%20analysis%20of%20plant%20samples..pdf |
| _version_ | 1848846254865383424 |
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| author | Yang, Cui Ren, Chunyan Piao, Xiangfan Narayanan, Kannan Li, Donghao |
| author_facet | Yang, Cui Ren, Chunyan Piao, Xiangfan Narayanan, Kannan Li, Donghao |
| author_sort | Yang, Cui |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A continuous-flow, on-line sample pretreatment technique using a silica gel microsyringe extractor has been developed. All steps including extraction, separation, clean-up, and concentration occur in the microsyringe. The overall sample pretreatment process takes <10 min per sample. Different polarity chemicals in the plant sample are successively extracted and separated, and analyzed in parallel using HPLC–UV and HPLC–UV–MS/MS. Polycyclic aromatic hydrocarbons, alkylphenols, and plant hormones were determined as model compounds for nonpolar, intermediate polarity, and polar fractions, respectively. All the parameters that influence the extraction and separation efficiency of the microsyringe extractor have been optimized and evaluated. Under the optimized conditions, recoveries of target compounds ranged from 78.4 to 101.9%, the RSD was <12.8% and the square of the correlation coefficient was >0.99. Complex plant samples of Sambucus Mandshurica Kitag have been tested using this method. Fluorene, phenanthrene, pyrene, and plant hormones were detected in all the samples, and concentrations ranged from 24.2–34.9, 43.8–67.1, 25.9–29.2, and 14.5∼110.8 ng/g, respectively. |
| first_indexed | 2025-11-15T08:59:48Z |
| format | Article |
| id | upm-28936 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:59:48Z |
| publishDate | 2013 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-289362015-10-26T02:52:16Z http://psasir.upm.edu.my/id/eprint/28936/ An on-line sample pretreatment technique for the HPLC analysis of plant samples. Yang, Cui Ren, Chunyan Piao, Xiangfan Narayanan, Kannan Li, Donghao A continuous-flow, on-line sample pretreatment technique using a silica gel microsyringe extractor has been developed. All steps including extraction, separation, clean-up, and concentration occur in the microsyringe. The overall sample pretreatment process takes <10 min per sample. Different polarity chemicals in the plant sample are successively extracted and separated, and analyzed in parallel using HPLC–UV and HPLC–UV–MS/MS. Polycyclic aromatic hydrocarbons, alkylphenols, and plant hormones were determined as model compounds for nonpolar, intermediate polarity, and polar fractions, respectively. All the parameters that influence the extraction and separation efficiency of the microsyringe extractor have been optimized and evaluated. Under the optimized conditions, recoveries of target compounds ranged from 78.4 to 101.9%, the RSD was <12.8% and the square of the correlation coefficient was >0.99. Complex plant samples of Sambucus Mandshurica Kitag have been tested using this method. Fluorene, phenanthrene, pyrene, and plant hormones were detected in all the samples, and concentrations ranged from 24.2–34.9, 43.8–67.1, 25.9–29.2, and 14.5∼110.8 ng/g, respectively. Wiley 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28936/1/An%20on-line%20sample%20pretreatment%20technique%20for%20the%20HPLC%20analysis%20of%20plant%20samples..pdf Yang, Cui and Ren, Chunyan and Piao, Xiangfan and Narayanan, Kannan and Li, Donghao (2013) An on-line sample pretreatment technique for the HPLC analysis of plant samples. Journal of Separation Science, 36 (21-22). pp. 3599-3607. ISSN 1615-9306; ESSN: 1615-9314 10.1002/jssc.201300722 English |
| spellingShingle | Yang, Cui Ren, Chunyan Piao, Xiangfan Narayanan, Kannan Li, Donghao An on-line sample pretreatment technique for the HPLC analysis of plant samples. |
| title | An on-line sample pretreatment technique for the HPLC analysis of plant samples. |
| title_full | An on-line sample pretreatment technique for the HPLC analysis of plant samples. |
| title_fullStr | An on-line sample pretreatment technique for the HPLC analysis of plant samples. |
| title_full_unstemmed | An on-line sample pretreatment technique for the HPLC analysis of plant samples. |
| title_short | An on-line sample pretreatment technique for the HPLC analysis of plant samples. |
| title_sort | on-line sample pretreatment technique for the hplc analysis of plant samples. |
| url | http://psasir.upm.edu.my/id/eprint/28936/ http://psasir.upm.edu.my/id/eprint/28936/ http://psasir.upm.edu.my/id/eprint/28936/1/An%20on-line%20sample%20pretreatment%20technique%20for%20the%20HPLC%20analysis%20of%20plant%20samples..pdf |