Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters
Lipase from Candida rugosa was immobilized on three different supports, i.e. Amberlite XAD7, poly(methylmethacrylate) (PMMA) and celite. With the conditions tested, maximum adsorption can be achieved after 30 min. The activities of the immobilized lipases were determined by the esterification reacti...
| _version_ | 1848846250425712640 |
|---|---|
| author | Basri, Mahiran Wan Yunus, Wan Md. Zin Wong, Soo Yoong Ampon, Kamaruzaman Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_facet | Basri, Mahiran Wan Yunus, Wan Md. Zin Wong, Soo Yoong Ampon, Kamaruzaman Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_sort | Basri, Mahiran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lipase from Candida rugosa was immobilized on three different supports, i.e. Amberlite XAD7, poly(methylmethacrylate) (PMMA) and celite. With the conditions tested, maximum adsorption can be achieved after 30 min. The activities of the immobilized lipases were determined by the esterification reaction of oleic acid and butanol. The immobilized lipases were found to be very effective in the esterification reaction. The immobilized activities generally were high in apolar organic solvents with log P values from 2·0 to 4·0. The preference for fatty acids as acyl donors differed in all cases of immobilized lipases. Lipase immobilized on XAD7 and PMMA exhibited high preference of acyl donors (fatty acids) with chain lengths 12–18 and 8–18, respectively. Lipase immobilized on celite, however, showed high activity in all cases of fatty acids. The nucleophile (alcohol) selectivity studies showed that lipase immobilized on XAD7 and celite was more accessible to alcohols of chain lengths 3–12. However, lipase immobilized on PMMA showed a significant preference towards alcohols of chain lengths from 3 to 10. |
| first_indexed | 2025-11-15T08:59:43Z |
| format | Article |
| id | upm-28921 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:59:43Z |
| publishDate | 1996 |
| publisher | Society of Chemical Industry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-289212024-08-05T02:46:50Z http://psasir.upm.edu.my/id/eprint/28921/ Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters Basri, Mahiran Wan Yunus, Wan Md. Zin Wong, Soo Yoong Ampon, Kamaruzaman Abdul Razak, Che Nyonya Salleh, Abu Bakar Lipase from Candida rugosa was immobilized on three different supports, i.e. Amberlite XAD7, poly(methylmethacrylate) (PMMA) and celite. With the conditions tested, maximum adsorption can be achieved after 30 min. The activities of the immobilized lipases were determined by the esterification reaction of oleic acid and butanol. The immobilized lipases were found to be very effective in the esterification reaction. The immobilized activities generally were high in apolar organic solvents with log P values from 2·0 to 4·0. The preference for fatty acids as acyl donors differed in all cases of immobilized lipases. Lipase immobilized on XAD7 and PMMA exhibited high preference of acyl donors (fatty acids) with chain lengths 12–18 and 8–18, respectively. Lipase immobilized on celite, however, showed high activity in all cases of fatty acids. The nucleophile (alcohol) selectivity studies showed that lipase immobilized on XAD7 and celite was more accessible to alcohols of chain lengths 3–12. However, lipase immobilized on PMMA showed a significant preference towards alcohols of chain lengths from 3 to 10. Society of Chemical Industry 1996-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/28921/1/Immobilization%20of%20lipase%20from%20Candida%20rugosa%20on%20synthetic%20polymer%20beads%20for%20use%20in%20the%20synthesis%20of%20fatty%20esters.pdf text en http://psasir.upm.edu.my/id/eprint/28921/7/J%20of%20Chemical%20Tech%20%20%20Biotech%20-%20June%201996%20-%20Basri%20-%20Immobilization%20of%20lipase%20from%20Candida%20rugosa%20on%20synthetic%20polymer%20beads.pdf Basri, Mahiran and Wan Yunus, Wan Md. Zin and Wong, Soo Yoong and Ampon, Kamaruzaman and Abdul Razak, Che Nyonya and Salleh, Abu Bakar (1996) Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters. Journal of Chemical Technology and Biotechnology, 66 (2). pp. 169-173. ISSN 0268-2575; ESSN: 1097-4660 http://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291097-4660%28199606%2966:2%3C169::AID-JCTB478%3E3.0.CO;2-I/abstract;jsessionid=6A34374047D91FF7F421E895C67A3812.f02t01 10.1002/(SICI)1097-4660(199606)66:2<169::AID-JCTB478>3.0.CO;2-I |
| spellingShingle | Basri, Mahiran Wan Yunus, Wan Md. Zin Wong, Soo Yoong Ampon, Kamaruzaman Abdul Razak, Che Nyonya Salleh, Abu Bakar Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters |
| title | Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters |
| title_full | Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters |
| title_fullStr | Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters |
| title_full_unstemmed | Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters |
| title_short | Immobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters |
| title_sort | immobilization of lipase from candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters |
| url | http://psasir.upm.edu.my/id/eprint/28921/ http://psasir.upm.edu.my/id/eprint/28921/ http://psasir.upm.edu.my/id/eprint/28921/ http://psasir.upm.edu.my/id/eprint/28921/1/Immobilization%20of%20lipase%20from%20Candida%20rugosa%20on%20synthetic%20polymer%20beads%20for%20use%20in%20the%20synthesis%20of%20fatty%20esters.pdf http://psasir.upm.edu.my/id/eprint/28921/7/J%20of%20Chemical%20Tech%20%20%20Biotech%20-%20June%201996%20-%20Basri%20-%20Immobilization%20of%20lipase%20from%20Candida%20rugosa%20on%20synthetic%20polymer%20beads.pdf |