Proses Penghasilan Bromelin daripada Batang Nenas

A proteolytic enzyme called bromelin can be produced from pineapple stem by precipitating it from pineapple stem juice and separating the precipitate from the juice. Acetone is used as the precipitant. The precipitating process is performed twice and the second precipitate is taken as the bromelin...

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Main Authors: Wan Daud, Wan Ramli, Yusoff, Mohd. Fauzi, Krishna Rao, D. S
Format: Article
Language:English
English
Published: 1990
Online Access:http://psasir.upm.edu.my/id/eprint/2885/
http://psasir.upm.edu.my/id/eprint/2885/1/Proses_Penghasilan_Bromelin_daripada_Batang_Nenas.pdf
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author Wan Daud, Wan Ramli
Yusoff, Mohd. Fauzi
Krishna Rao, D. S
author_facet Wan Daud, Wan Ramli
Yusoff, Mohd. Fauzi
Krishna Rao, D. S
author_sort Wan Daud, Wan Ramli
building UPM Institutional Repository
collection Online Access
description A proteolytic enzyme called bromelin can be produced from pineapple stem by precipitating it from pineapple stem juice and separating the precipitate from the juice. Acetone is used as the precipitant. The precipitating process is performed twice and the second precipitate is taken as the bromelin product. This process is not well understood and is not amenable to mathematical modelling. The main aim of the present study is to identify process variables such as the precipitant: juice ratio, precipitation time and centrifugation time in every stage which have a significant effect on the yield and activity of bromelin. Factor analysis is used. This study found that the centrifugation times in both stages, precipitation and centrifugation times in the second stage affect the yield of bromelin. The enzyme activity is affected by the precipitant: juice ratio, the precipitation and centrifugation times in the first stage,' precipitant: juice ratio and precipitation time in the second stage. This process is currently being optimised.
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institution Universiti Putra Malaysia
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language English
English
last_indexed 2025-11-15T07:10:25Z
publishDate 1990
recordtype eprints
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spelling upm-28852013-05-27T07:04:10Z http://psasir.upm.edu.my/id/eprint/2885/ Proses Penghasilan Bromelin daripada Batang Nenas Wan Daud, Wan Ramli Yusoff, Mohd. Fauzi Krishna Rao, D. S A proteolytic enzyme called bromelin can be produced from pineapple stem by precipitating it from pineapple stem juice and separating the precipitate from the juice. Acetone is used as the precipitant. The precipitating process is performed twice and the second precipitate is taken as the bromelin product. This process is not well understood and is not amenable to mathematical modelling. The main aim of the present study is to identify process variables such as the precipitant: juice ratio, precipitation time and centrifugation time in every stage which have a significant effect on the yield and activity of bromelin. Factor analysis is used. This study found that the centrifugation times in both stages, precipitation and centrifugation times in the second stage affect the yield of bromelin. The enzyme activity is affected by the precipitant: juice ratio, the precipitation and centrifugation times in the first stage,' precipitant: juice ratio and precipitation time in the second stage. This process is currently being optimised. 1990 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/2885/1/Proses_Penghasilan_Bromelin_daripada_Batang_Nenas.pdf Wan Daud, Wan Ramli and Yusoff, Mohd. Fauzi and Krishna Rao, D. S (1990) Proses Penghasilan Bromelin daripada Batang Nenas. Pertanika, 13 (1). pp. 113-121. English
spellingShingle Wan Daud, Wan Ramli
Yusoff, Mohd. Fauzi
Krishna Rao, D. S
Proses Penghasilan Bromelin daripada Batang Nenas
title Proses Penghasilan Bromelin daripada Batang Nenas
title_full Proses Penghasilan Bromelin daripada Batang Nenas
title_fullStr Proses Penghasilan Bromelin daripada Batang Nenas
title_full_unstemmed Proses Penghasilan Bromelin daripada Batang Nenas
title_short Proses Penghasilan Bromelin daripada Batang Nenas
title_sort proses penghasilan bromelin daripada batang nenas
url http://psasir.upm.edu.my/id/eprint/2885/
http://psasir.upm.edu.my/id/eprint/2885/1/Proses_Penghasilan_Bromelin_daripada_Batang_Nenas.pdf