Identification of Nasonov Pheromones and the Effects of Synthetic Pheromones on the Clustering Activity of the Asiatic Honeybee (APis Cerana)
Worker bees from several Apis cerana colonies were sampled for identification ofNasonov secretions and their clustering activity in 1°esponse to different synthetic components of the swarming pheromones. Extraction of Nasonov gland secretion was canied (mt try three methods - Abdomen Dippingmethod...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
1980
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| Online Access: | http://psasir.upm.edu.my/id/eprint/2842/ http://psasir.upm.edu.my/id/eprint/2842/1/Identification_of_Nasonov_Pheromones_and_the_Effects.pdf |
| Summary: | Worker bees from several Apis cerana colonies were sampled for identification ofNasonov secretions and their
clustering activity in 1°esponse to different synthetic components of the swarming pheromones. Extraction of
Nasonov gland secretion was canied (mt try three methods - Abdomen Dippingmethod, Excised Gland method and
S)'ringe Extmction method - and its anal),sis was canied out b), Gas Liquid Chromatograph),. Nasonov
components delected were (E)-citml, (Z)-citml and g~ranioL However, onl), (E)-citml and geraniol were
quantified. The Abdoment Dipping method detected 20.0 ug per bee oj (E) -citral, whereas the Excised Gland
method detected onl), 3.8 ug. Geraniol was onI)' detected try the latter technique at 2.5 ng Plff bee. (Z) -citral was
detected in t·race amounts. The clustering experiments were conducted in a confined darkened room at room
temperat1m. Chemical components, consisting of 9-oxo-2-decenoic acid designated as 9ODA, (E), (Z)-citral,
geraniol, nerol andfarnesol, were tested either singly or in vmious combinations. Each component or mixtures of
these componenls were adsorbed on afilterpaperplaced in alighted peifomted ca,.dboard cannister. Five cannislen
were used in each tlial. Among the synthetic compon""ts tested, the (E), (Z) -citral was[oltnd to be most effective
in attracting w01*er bees to fonn a clustero |
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