Morphological and molecular datasets for Kaempferia species

This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenB...

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Main Authors: Labrooy, Catherine Dharshini, Abdullah, Thohirah Lee, Stanslas, Johnson
Format: Article
Language:English
Published: Elsevier 2018
Online Access:http://psasir.upm.edu.my/id/eprint/28148/
http://psasir.upm.edu.my/id/eprint/28148/1/28148.pdf
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author Labrooy, Catherine Dharshini
Abdullah, Thohirah Lee
Stanslas, Johnson
author_facet Labrooy, Catherine Dharshini
Abdullah, Thohirah Lee
Stanslas, Johnson
author_sort Labrooy, Catherine Dharshini
building UPM Institutional Repository
collection Online Access
description This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenBank accessions. DNA was extracted using a modified CTAB method and PCR amplification was completed using Internal Transcribed Spacer (ITS4 and ITS5) markers. PCR amplification of products were viewed under gel electrophoresis. Sequencing was performed and sequence characteristics of ITS rDNA in Kaempferia is shown. Qualitative and qualitative scoring of morphological characters and measuring techniques for Kaempferia species are included. In addition, a brief review of molecular markers used in phylogenetic studies of Zingiberaceae is included in this dataset.
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spelling upm-281482020-04-15T16:12:57Z http://psasir.upm.edu.my/id/eprint/28148/ Morphological and molecular datasets for Kaempferia species Labrooy, Catherine Dharshini Abdullah, Thohirah Lee Stanslas, Johnson This study compared morphological and molecular data for identification of Kaempferia species. Each species was deposited in Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) as voucher specimens and ITS sequences of each species deposited in NCBI (https://www.ncbi.nlm.nih.gov/) as GenBank accessions. DNA was extracted using a modified CTAB method and PCR amplification was completed using Internal Transcribed Spacer (ITS4 and ITS5) markers. PCR amplification of products were viewed under gel electrophoresis. Sequencing was performed and sequence characteristics of ITS rDNA in Kaempferia is shown. Qualitative and qualitative scoring of morphological characters and measuring techniques for Kaempferia species are included. In addition, a brief review of molecular markers used in phylogenetic studies of Zingiberaceae is included in this dataset. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/28148/1/28148.pdf Labrooy, Catherine Dharshini and Abdullah, Thohirah Lee and Stanslas, Johnson (2018) Morphological and molecular datasets for Kaempferia species. Data in Brief, 21. pp. 1678-1685. ISSN 2352-3409 https://www.sciencedirect.com/science/article/pii/S2352340918313143#! 10.1016/j.dib.2018.10.097
spellingShingle Labrooy, Catherine Dharshini
Abdullah, Thohirah Lee
Stanslas, Johnson
Morphological and molecular datasets for Kaempferia species
title Morphological and molecular datasets for Kaempferia species
title_full Morphological and molecular datasets for Kaempferia species
title_fullStr Morphological and molecular datasets for Kaempferia species
title_full_unstemmed Morphological and molecular datasets for Kaempferia species
title_short Morphological and molecular datasets for Kaempferia species
title_sort morphological and molecular datasets for kaempferia species
url http://psasir.upm.edu.my/id/eprint/28148/
http://psasir.upm.edu.my/id/eprint/28148/
http://psasir.upm.edu.my/id/eprint/28148/
http://psasir.upm.edu.my/id/eprint/28148/1/28148.pdf