Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation.
oniothalamin is a biologically active styrylpyrone derivative isolated from various Goniothalamus species. The ability of goniothalamin to induce apoptosis via caspase-3 activation against hepatoblastoma (HepG2) and normal liver cells (Chang cells) was studied using morphological and biochemical eva...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
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Taylor & Francis
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28113/ http://psasir.upm.edu.my/id/eprint/28113/1/Goniothalamin%20selectively%20induces%20apoptosis%20on%20human%20hepatoblastoma%20cells%20through%20caspase.pdf |
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| author | Al-Qubaisi, Mothanna Rosli, Rozita Subramani, Tamilselvan Omar, Abdul Rahman Yeap, Swee Keong Ali, Abdul Manaf Mohamed Alitheen, Noorjahan Banu |
| author_facet | Al-Qubaisi, Mothanna Rosli, Rozita Subramani, Tamilselvan Omar, Abdul Rahman Yeap, Swee Keong Ali, Abdul Manaf Mohamed Alitheen, Noorjahan Banu |
| author_sort | Al-Qubaisi, Mothanna |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | oniothalamin is a biologically active styrylpyrone derivative isolated from various Goniothalamus species. The ability of goniothalamin to induce apoptosis via caspase-3 activation against hepatoblastoma (HepG2) and normal liver cells (Chang cells) was studied using morphological and biochemical evaluations. HepG2 and Chang cells were treated with goniothalamin for 72 h and analysed by TUNEL and Annexin-V/PI staining. Furthermore, the post-mitochondrial caspase-3 was quantified using ELISA. In view of our results, goniothalamin induced apoptosis on treated cells via alteration of cellular membrane integrity and cleavage of DNA. On the other hand, post-mitochondrial caspase-3 activity was significantly elevated in HepG2 cells treated with goniothalamin after 72 h. These findings suggest that goniothalamin induced apoptosis on HepG2 liver cancer cells via induction of caspase-3 with less sensitivity on the cell line of Chang cells. |
| first_indexed | 2025-11-15T08:56:10Z |
| format | Article |
| id | upm-28113 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:56:10Z |
| publishDate | 2013 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-281132015-09-10T08:25:38Z http://psasir.upm.edu.my/id/eprint/28113/ Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. Al-Qubaisi, Mothanna Rosli, Rozita Subramani, Tamilselvan Omar, Abdul Rahman Yeap, Swee Keong Ali, Abdul Manaf Mohamed Alitheen, Noorjahan Banu oniothalamin is a biologically active styrylpyrone derivative isolated from various Goniothalamus species. The ability of goniothalamin to induce apoptosis via caspase-3 activation against hepatoblastoma (HepG2) and normal liver cells (Chang cells) was studied using morphological and biochemical evaluations. HepG2 and Chang cells were treated with goniothalamin for 72 h and analysed by TUNEL and Annexin-V/PI staining. Furthermore, the post-mitochondrial caspase-3 was quantified using ELISA. In view of our results, goniothalamin induced apoptosis on treated cells via alteration of cellular membrane integrity and cleavage of DNA. On the other hand, post-mitochondrial caspase-3 activity was significantly elevated in HepG2 cells treated with goniothalamin after 72 h. These findings suggest that goniothalamin induced apoptosis on HepG2 liver cancer cells via induction of caspase-3 with less sensitivity on the cell line of Chang cells. Taylor & Francis 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28113/1/Goniothalamin%20selectively%20induces%20apoptosis%20on%20human%20hepatoblastoma%20cells%20through%20caspase.pdf Al-Qubaisi, Mothanna and Rosli, Rozita and Subramani, Tamilselvan and Omar, Abdul Rahman and Yeap, Swee Keong and Ali, Abdul Manaf and Mohamed Alitheen, Noorjahan Banu (2013) Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. Natural Product Research, 27 (23). pp. 2216-2218. ISSN 1478-6419 10.1080/14786419.2013.800979 English |
| spellingShingle | Al-Qubaisi, Mothanna Rosli, Rozita Subramani, Tamilselvan Omar, Abdul Rahman Yeap, Swee Keong Ali, Abdul Manaf Mohamed Alitheen, Noorjahan Banu Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. |
| title | Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. |
| title_full | Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. |
| title_fullStr | Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. |
| title_full_unstemmed | Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. |
| title_short | Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. |
| title_sort | goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. |
| url | http://psasir.upm.edu.my/id/eprint/28113/ http://psasir.upm.edu.my/id/eprint/28113/ http://psasir.upm.edu.my/id/eprint/28113/1/Goniothalamin%20selectively%20induces%20apoptosis%20on%20human%20hepatoblastoma%20cells%20through%20caspase.pdf |