Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis.
Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (G...
| Main Authors: | Baradaran, Ali, Sieo, Chin Chin, Foo, Hooi Ling, Md. Illias, Rosli, Yusoff, Khatijah, Abdul Rahim, Raha |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Springer Netherlands
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/28111/ http://psasir.upm.edu.my/id/eprint/28111/1/Cloning%20and%20in%20silico%20characterization%20of%20two%20signal%20peptides%20from%20Pediococcus%20pentosaceus%20and%20their%20function%20for%20the%20secretion%20of%20heterologous%20protein%20in%20Lactococcus%20lactis.pdf |
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