Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis.
Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (G...
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| Format: | Article |
| Language: | English English |
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Springer Netherlands
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28111/ http://psasir.upm.edu.my/id/eprint/28111/1/Cloning%20and%20in%20silico%20characterization%20of%20two%20signal%20peptides%20from%20Pediococcus%20pentosaceus%20and%20their%20function%20for%20the%20secretion%20of%20heterologous%20protein%20in%20Lactococcus%20lactis.pdf |
| _version_ | 1848846025893085184 |
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| author | Baradaran, Ali Sieo, Chin Chin Foo, Hooi Ling Md. Illias, Rosli Yusoff, Khatijah Abdul Rahim, Raha |
| author_facet | Baradaran, Ali Sieo, Chin Chin Foo, Hooi Ling Md. Illias, Rosli Yusoff, Khatijah Abdul Rahim, Raha |
| author_sort | Baradaran, Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (GFP) and cloned into Lactococcus lactis pNZ8048 and pMG36e vectors, respectively. Western blot analysis indicated that the GFP proteins were secreted using both heterologous SPs. ELISA showed that the secretion efficiency of GFP using Spk1 (0.64 μg/ml) was similar to using Usp45 (0.62 μg/ml) and Spk3 (0.58 μg/ml). |
| first_indexed | 2025-11-15T08:56:09Z |
| format | Article |
| id | upm-28111 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:56:09Z |
| publishDate | 2013 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-281112015-08-24T02:07:14Z http://psasir.upm.edu.my/id/eprint/28111/ Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. Baradaran, Ali Sieo, Chin Chin Foo, Hooi Ling Md. Illias, Rosli Yusoff, Khatijah Abdul Rahim, Raha Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (GFP) and cloned into Lactococcus lactis pNZ8048 and pMG36e vectors, respectively. Western blot analysis indicated that the GFP proteins were secreted using both heterologous SPs. ELISA showed that the secretion efficiency of GFP using Spk1 (0.64 μg/ml) was similar to using Usp45 (0.62 μg/ml) and Spk3 (0.58 μg/ml). Springer Netherlands 2013-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28111/1/Cloning%20and%20in%20silico%20characterization%20of%20two%20signal%20peptides%20from%20Pediococcus%20pentosaceus%20and%20their%20function%20for%20the%20secretion%20of%20heterologous%20protein%20in%20Lactococcus%20lactis.pdf Baradaran, Ali and Sieo, Chin Chin and Foo, Hooi Ling and Md. Illias, Rosli and Yusoff, Khatijah and Abdul Rahim, Raha (2013) Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. Biotechnology Letters, 35 (2). pp. 233-238. ISSN 0141-5492; ESSN: 1573-6776 10.1007/s10529-012-1059-4 English |
| spellingShingle | Baradaran, Ali Sieo, Chin Chin Foo, Hooi Ling Md. Illias, Rosli Yusoff, Khatijah Abdul Rahim, Raha Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. |
| title | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. |
| title_full | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. |
| title_fullStr | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. |
| title_full_unstemmed | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. |
| title_short | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. |
| title_sort | cloning and in silico characterization of two signal peptides from pediococcus pentosaceus and their function for the secretion of heterologous protein in lactococcus lactis. |
| url | http://psasir.upm.edu.my/id/eprint/28111/ http://psasir.upm.edu.my/id/eprint/28111/ http://psasir.upm.edu.my/id/eprint/28111/1/Cloning%20and%20in%20silico%20characterization%20of%20two%20signal%20peptides%20from%20Pediococcus%20pentosaceus%20and%20their%20function%20for%20the%20secretion%20of%20heterologous%20protein%20in%20Lactococcus%20lactis.pdf |