A simple and effective method for RNA isolation and cDNA library construction from the brown seaweed Sargassum polycystum (Fucales, Phaeophyceae)
The isolation of high-quality RNA from brown seaweeds has always been problematic due to a high content of polyphenolic compounds and polysaccharides in their tissues. This study presents a simple and effective method for isolating high-quality RNA from the brown seaweeds Sargassum (Sargassum polycy...
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| Format: | Article |
| Language: | English |
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Springer
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28106/ http://psasir.upm.edu.my/id/eprint/28106/1/A%20simple%20and%20effective%20method%20for%20RNA%20isolation%20and%20cDNA%20library%20construction%20from%20the%20brown%20seaweed%20Sargassum%20polycystum%20%28Fucales%2C%20Phaeophyceae%29.pdf |
| _version_ | 1848846024484847616 |
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| author | Sim, Mei Chea Ho, Chai Ling Phang, Siew Moi |
| author_facet | Sim, Mei Chea Ho, Chai Ling Phang, Siew Moi |
| author_sort | Sim, Mei Chea |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The isolation of high-quality RNA from brown seaweeds has always been problematic due to a high content of polyphenolic compounds and polysaccharides in their tissues. This study presents a simple and effective method for isolating high-quality RNA from the brown seaweeds Sargassum (Sargassum polycystum, Sargassum siliquosum, Sargassum baccularia, and Sargassum binderi), Padina tetrastromatica, Turbinaria conoides, Dictyota sp., and Hormophysa cuneiformis using cetyltrimethylammonium bromide and selective lithium chloride precipitation. Approximately 25–43 μg g−1 fresh weight of total RNA was obtained from these brown algae. The A 260/A 280 absorbance ratio of these RNA samples are between 1.995 ± 0.027 and 2.122 ± 0.013, with distinct 28S and 18S ribosomal RNA bands visible on a formaldehyde agarose gel. The RNA obtained from S. polycystum was found to be suitable for many downstream applications such as reverse transcription polymerase chain reaction, cloning, synthesis of cDNA, and construction of cDNA library for the generation of expressed sequence tags. |
| first_indexed | 2025-11-15T08:56:08Z |
| format | Article |
| id | upm-28106 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:56:08Z |
| publishDate | 2013 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-281062016-06-20T06:39:24Z http://psasir.upm.edu.my/id/eprint/28106/ A simple and effective method for RNA isolation and cDNA library construction from the brown seaweed Sargassum polycystum (Fucales, Phaeophyceae) Sim, Mei Chea Ho, Chai Ling Phang, Siew Moi The isolation of high-quality RNA from brown seaweeds has always been problematic due to a high content of polyphenolic compounds and polysaccharides in their tissues. This study presents a simple and effective method for isolating high-quality RNA from the brown seaweeds Sargassum (Sargassum polycystum, Sargassum siliquosum, Sargassum baccularia, and Sargassum binderi), Padina tetrastromatica, Turbinaria conoides, Dictyota sp., and Hormophysa cuneiformis using cetyltrimethylammonium bromide and selective lithium chloride precipitation. Approximately 25–43 μg g−1 fresh weight of total RNA was obtained from these brown algae. The A 260/A 280 absorbance ratio of these RNA samples are between 1.995 ± 0.027 and 2.122 ± 0.013, with distinct 28S and 18S ribosomal RNA bands visible on a formaldehyde agarose gel. The RNA obtained from S. polycystum was found to be suitable for many downstream applications such as reverse transcription polymerase chain reaction, cloning, synthesis of cDNA, and construction of cDNA library for the generation of expressed sequence tags. Springer 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28106/1/A%20simple%20and%20effective%20method%20for%20RNA%20isolation%20and%20cDNA%20library%20construction%20from%20the%20brown%20seaweed%20Sargassum%20polycystum%20%28Fucales%2C%20Phaeophyceae%29.pdf Sim, Mei Chea and Ho, Chai Ling and Phang, Siew Moi (2013) A simple and effective method for RNA isolation and cDNA library construction from the brown seaweed Sargassum polycystum (Fucales, Phaeophyceae). Journal of Applied Phycology, 25 (5). pp. 1277-1285. ISSN 0921-8971; ESSN: 1573-5176 http://link.springer.com/article/10.1007%2Fs10811-013-9980-z 10.1007/s10811-013-9980-z |
| spellingShingle | Sim, Mei Chea Ho, Chai Ling Phang, Siew Moi A simple and effective method for RNA isolation and cDNA library construction from the brown seaweed Sargassum polycystum (Fucales, Phaeophyceae) |
| title | A simple and effective method for RNA isolation and cDNA library construction from the brown seaweed Sargassum polycystum (Fucales, Phaeophyceae) |
| title_full | A simple and effective method for RNA isolation and cDNA library construction from the brown seaweed Sargassum polycystum (Fucales, Phaeophyceae) |
| title_fullStr | A simple and effective method for RNA isolation and cDNA library construction from the brown seaweed Sargassum polycystum (Fucales, Phaeophyceae) |
| title_full_unstemmed | A simple and effective method for RNA isolation and cDNA library construction from the brown seaweed Sargassum polycystum (Fucales, Phaeophyceae) |
| title_short | A simple and effective method for RNA isolation and cDNA library construction from the brown seaweed Sargassum polycystum (Fucales, Phaeophyceae) |
| title_sort | simple and effective method for rna isolation and cdna library construction from the brown seaweed sargassum polycystum (fucales, phaeophyceae) |
| url | http://psasir.upm.edu.my/id/eprint/28106/ http://psasir.upm.edu.my/id/eprint/28106/ http://psasir.upm.edu.my/id/eprint/28106/ http://psasir.upm.edu.my/id/eprint/28106/1/A%20simple%20and%20effective%20method%20for%20RNA%20isolation%20and%20cDNA%20library%20construction%20from%20the%20brown%20seaweed%20Sargassum%20polycystum%20%28Fucales%2C%20Phaeophyceae%29.pdf |