Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems

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Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems

The purification of intracellular human recombinant interferon-alpha2b (IFN-α2b) from Escherichia coli (E. coli) was studied using alcohol/salt aqueous two-phase system (ATPS). The influences of nine biphasic systems comprising alcohol-based top phase (ethanol, 1-propanol and 2-propanol) and salt-ba...

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Bibliographic Details
Main Authors:	Lin, Yu Kiat, Ooi, Chien Wei, Tan, Joo Shun, Pau, Loke Show, Ariff, Arbakariya, Ling, Tau Chuan
Format:	Article
Language:	English
Published:	Elsevier 2013
Online Access:	http://psasir.upm.edu.my/id/eprint/28099/ http://psasir.upm.edu.my/id/eprint/28099/1/Recovery%20of%20human%20interferon%20alpha-2b%20from%20recombinant%20Escherichia%20coli%20using%20alcoholsalt-based%20aqueous%20two-phase%20systems.pdf

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