Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems
The purification of intracellular human recombinant interferon-alpha2b (IFN-α2b) from Escherichia coli (E. coli) was studied using alcohol/salt aqueous two-phase system (ATPS). The influences of nine biphasic systems comprising alcohol-based top phase (ethanol, 1-propanol and 2-propanol) and salt-ba...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/28099/ http://psasir.upm.edu.my/id/eprint/28099/1/Recovery%20of%20human%20interferon%20alpha-2b%20from%20recombinant%20Escherichia%20coli%20using%20alcoholsalt-based%20aqueous%20two-phase%20systems.pdf |
| _version_ | 1848846022534496256 |
|---|---|
| author | Lin, Yu Kiat Ooi, Chien Wei Tan, Joo Shun Pau, Loke Show Ariff, Arbakariya Ling, Tau Chuan |
| author_facet | Lin, Yu Kiat Ooi, Chien Wei Tan, Joo Shun Pau, Loke Show Ariff, Arbakariya Ling, Tau Chuan |
| author_sort | Lin, Yu Kiat |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The purification of intracellular human recombinant interferon-alpha2b (IFN-α2b) from Escherichia coli (E. coli) was studied using alcohol/salt aqueous two-phase system (ATPS). The influences of nine biphasic systems comprising alcohol-based top phase (ethanol, 1-propanol and 2-propanol) and salt-based bottom phase (ammonium sulfate, di-potassium hydrogen phosphate and monosodium citrate) on IFN-α2b purification were studied. The results showed that the optimum condition for purification of IFN-α2b was achieved in ATPS composed of 18% (w/w) 2-propanol with 22% (w/w) ammonium sulfate in the presence of 1% (w/w) sodium chloride (NaCl). The purified IFN-α2b recorded a purification factor (PF) of 16.24 with the yield of 74.64%. |
| first_indexed | 2025-11-15T08:56:06Z |
| format | Article |
| id | upm-28099 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:56:06Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280992016-06-20T05:55:56Z http://psasir.upm.edu.my/id/eprint/28099/ Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems Lin, Yu Kiat Ooi, Chien Wei Tan, Joo Shun Pau, Loke Show Ariff, Arbakariya Ling, Tau Chuan The purification of intracellular human recombinant interferon-alpha2b (IFN-α2b) from Escherichia coli (E. coli) was studied using alcohol/salt aqueous two-phase system (ATPS). The influences of nine biphasic systems comprising alcohol-based top phase (ethanol, 1-propanol and 2-propanol) and salt-based bottom phase (ammonium sulfate, di-potassium hydrogen phosphate and monosodium citrate) on IFN-α2b purification were studied. The results showed that the optimum condition for purification of IFN-α2b was achieved in ATPS composed of 18% (w/w) 2-propanol with 22% (w/w) ammonium sulfate in the presence of 1% (w/w) sodium chloride (NaCl). The purified IFN-α2b recorded a purification factor (PF) of 16.24 with the yield of 74.64%. Elsevier 2013-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28099/1/Recovery%20of%20human%20interferon%20alpha-2b%20from%20recombinant%20Escherichia%20coli%20using%20alcoholsalt-based%20aqueous%20two-phase%20systems.pdf Lin, Yu Kiat and Ooi, Chien Wei and Tan, Joo Shun and Pau, Loke Show and Ariff, Arbakariya and Ling, Tau Chuan (2013) Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems. Separation and Purification Technology, 120. pp. 362-366. ISSN 1383-5866; ESSN: 1873-3794 http://www.sciencedirect.com/science/article/pii/S1383586613005753 10.1016/j.seppur.2013.09.038 |
| spellingShingle | Lin, Yu Kiat Ooi, Chien Wei Tan, Joo Shun Pau, Loke Show Ariff, Arbakariya Ling, Tau Chuan Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems |
| title | Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems |
| title_full | Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems |
| title_fullStr | Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems |
| title_full_unstemmed | Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems |
| title_short | Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems |
| title_sort | recovery of human interferon alpha-2b from recombinant escherichia coli using alcohol/salt-based aqueous two-phase systems |
| url | http://psasir.upm.edu.my/id/eprint/28099/ http://psasir.upm.edu.my/id/eprint/28099/ http://psasir.upm.edu.my/id/eprint/28099/ http://psasir.upm.edu.my/id/eprint/28099/1/Recovery%20of%20human%20interferon%20alpha-2b%20from%20recombinant%20Escherichia%20coli%20using%20alcoholsalt-based%20aqueous%20two-phase%20systems.pdf |