Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells
Mesenchymal stem cells have been increasingly introduced to have great potential in regenerative medicine, immunotherapy, and gene therapy due to their unique properties of self-renewal and differentiation into multiple cell lineages. Studies have shown that these properties may be limited and chang...
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| Format: | Article |
| Language: | English |
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Springer
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28093/ http://psasir.upm.edu.my/id/eprint/28093/1/28093.pdf |
| _version_ | 1848846020889280512 |
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| author | Salehinejad, Parvin Mohammed Alitheen, Noorjahan Banu Mandegary, Ali Nematollahi-Mahani, Seyed Noureddin Janzamin, Ehsan |
| author_facet | Salehinejad, Parvin Mohammed Alitheen, Noorjahan Banu Mandegary, Ali Nematollahi-Mahani, Seyed Noureddin Janzamin, Ehsan |
| author_sort | Salehinejad, Parvin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mesenchymal stem cells have been increasingly introduced to have great potential in regenerative medicine, immunotherapy, and gene therapy due to their unique properties of self-renewal and differentiation into multiple cell lineages. Studies have shown that these properties may be limited and changed by senescence-associated growth arrest under different culture conditions. This study aimed to present the ability of some growth factors on human umbilical cord mesenchymal (hUCM) cells expansion and telomerase activity. To optimize hUCM cell growth, epidermal growth factor (EGF) and fibroblast growth factor (FGF) were utilized in culture media, and the ability of these growth factors on the expression of the telomerase reverse transcriptase (TERT) gene and cell cycle phases was investigated. TERT mRNA expression increased in the hUCM cells treated by EGF and FGF. So, the untreated hUCM cells expressed 30.49 ± 7.15% of TERT, while EGF-treated cells expressed 51.82 ± 12.96% and FGF-treated cells expressed 33.77 ± 11.55% of TERT. Exposure of hUCM cells to EGF or FGF also promoted the progression of cells from G1 to S phase of the cell cycle and induced them to decrease the number of cells entering the G2/M phase. Our study showed that EGF and, to a lesser extent, FGF amplify the proliferation and expansion of hUCM cells. |
| first_indexed | 2025-11-15T08:56:05Z |
| format | Article |
| id | upm-28093 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:56:05Z |
| publishDate | 2013 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280932016-04-22T04:05:27Z http://psasir.upm.edu.my/id/eprint/28093/ Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells Salehinejad, Parvin Mohammed Alitheen, Noorjahan Banu Mandegary, Ali Nematollahi-Mahani, Seyed Noureddin Janzamin, Ehsan Mesenchymal stem cells have been increasingly introduced to have great potential in regenerative medicine, immunotherapy, and gene therapy due to their unique properties of self-renewal and differentiation into multiple cell lineages. Studies have shown that these properties may be limited and changed by senescence-associated growth arrest under different culture conditions. This study aimed to present the ability of some growth factors on human umbilical cord mesenchymal (hUCM) cells expansion and telomerase activity. To optimize hUCM cell growth, epidermal growth factor (EGF) and fibroblast growth factor (FGF) were utilized in culture media, and the ability of these growth factors on the expression of the telomerase reverse transcriptase (TERT) gene and cell cycle phases was investigated. TERT mRNA expression increased in the hUCM cells treated by EGF and FGF. So, the untreated hUCM cells expressed 30.49 ± 7.15% of TERT, while EGF-treated cells expressed 51.82 ± 12.96% and FGF-treated cells expressed 33.77 ± 11.55% of TERT. Exposure of hUCM cells to EGF or FGF also promoted the progression of cells from G1 to S phase of the cell cycle and induced them to decrease the number of cells entering the G2/M phase. Our study showed that EGF and, to a lesser extent, FGF amplify the proliferation and expansion of hUCM cells. Springer 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28093/1/28093.pdf Salehinejad, Parvin and Mohammed Alitheen, Noorjahan Banu and Mandegary, Ali and Nematollahi-Mahani, Seyed Noureddin and Janzamin, Ehsan (2013) Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells. In Vitro Cellular & Developmental Biology - Animal, 49 (7). pp. 515-523. ISSN 1071-2690; ESSN: 1543-706X http://link.springer.com/article/10.1007%2Fs11626-013-9631-3 10.1007/s11626-013-9631-3 |
| spellingShingle | Salehinejad, Parvin Mohammed Alitheen, Noorjahan Banu Mandegary, Ali Nematollahi-Mahani, Seyed Noureddin Janzamin, Ehsan Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells |
| title | Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells |
| title_full | Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells |
| title_fullStr | Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells |
| title_full_unstemmed | Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells |
| title_short | Effect of EGF and FGF on the expansion properties of human umbilical cord mesenchymal cells |
| title_sort | effect of egf and fgf on the expansion properties of human umbilical cord mesenchymal cells |
| url | http://psasir.upm.edu.my/id/eprint/28093/ http://psasir.upm.edu.my/id/eprint/28093/ http://psasir.upm.edu.my/id/eprint/28093/ http://psasir.upm.edu.my/id/eprint/28093/1/28093.pdf |